Identification and quantitative determination of a specific impurity of 4,4'-dithio-bis(2,6-tert-butylphenol) in probucol by reversed-phase HPLC

1996 ◽  
Vol 30 (7) ◽  
pp. 480-481
Author(s):  
S. V. Kuz'min ◽  
A. I. Chernyshev ◽  
V. P. Zhestkov
Keyword(s):  
Quantitative Determination ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Specific Impurity

Quantitative determination of itazigrel in rodent diet by reversed-phase HPLC and evaluation of its stability at 20°C

1989 ◽  
Vol 3 (4) ◽  
pp. 180-182
Author(s):  
Paul A. Bombardt ◽  
Brian E. Bothwell ◽  
Sharon K. Closson ◽  
Wade J. Adams
Keyword(s):  
Quantitative Determination ◽  
Reversed Phase ◽  
Reversed Phase Hplc

TLC-Densitometric and RP-HPLC Methods for Simultaneous Determination of Dexamethasone and Chlorpheniramine Maleate in the Presence of Methylparaben and Propylparaben

2017 ◽  
Vol 100 (1) ◽  
pp. 51-58
Author(s):  
Nehal F Farid ◽  
Ibrahim A Naguib ◽  
Radwa S Moatamed ◽  
Mohamed R El Ghobashy
Keyword(s):  
Particle Size ◽  
Quantitative Determination ◽  
Mobile Phase ◽  
Ammonium Acetate ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Chlorpheniramine Maleate ◽  
Ammonium Acetate Buffer ◽  
Rp Hplc

Abstract Validated simple, sensitive, and highly selective methods are applied for the quantitative determination ofdexamethasone and chlorpheniramine maleate in the presence of their reported preservatives (methylparaben and propylparaben), whether in pure forms or in pharmaceutical formulation. TLC is the first method, in which dexamethasone, chlorpheniramine maleate, methylparaben, and propylparaben are separated on silica gel TLC F254 plates using hexane–acetone–ammonia (5.5 + 4.5 + 0.5, v/v/v) as the developing phase. Separated bands are scanned at 254 nm over a concentration range of 0.1–1.7 and 0.4–2.8 μg/band, with mean ± SD recoveries of 99.12 ± 0.964 and 100.14 ± 0.962%, for dexamethasone and chlorpheniramine maleate, respectively. Reversed-phase HPLC is the second method, in which a mixture of dexamethasone and chlorpheniramine maleate, methylparaben, and propylparaben is separated on a reversed-phase silica C18 (5 μm particle size, 250 mm, 4.6 mm id) column using 0.1 M ammonium acetate buffer–acetonitrile (60 + 40, v/v, pH 3) as the mobile phase. The drugs were detected at 220 nm over a concentration range of 5–50 μg/mL, 2–90 μg/mL, 4–100 μg/mL, and 7–50μg/mL, with mean ± SD recoveries of 100.85 ± 0.905, 99.67 ± 1.281, 100.20 ± 0.906, and 99.81 ± 0.954%, for dexamethasone, chlorpheniramine maleate, methylparaben paraben, and propylparaben, respectively. The advantages of the suggested methods over previously reported methods are the ability to detect lower concentrations of the main drugs and to show better resolution of interfering preservatives; hence, these methods could be more reliable for routine QC analyses.

Quantitative determination of undeclared theobromine and theophylline in energy drinks by reversed-phase HPLC

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
A Uzunovic ◽  
A Sapcanin ◽  
A Elezovic ◽  
S Hadzidedic ◽  
S Pilipovic ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Energy Drinks ◽  
Reversed Phase ◽  
Reversed Phase Hplc
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