HPLC-analysis and preliminary pharmacokinetic parameter estimations of chloroquine

1981 ◽  
Vol 3 (1) ◽  
pp. 1575-1579
Author(s):  
B. Oosterhuis ◽  
M. Van Den Berg ◽  
J. Wetsteyn ◽  
C. J. Van Boxtel
Keyword(s):  
Pharmacokinetic Parameter ◽  
Hplc Analysis ◽  
Parameter Estimations

Drug Degradation during HPLC Analysis of Aztreonam: Effect on Pharmacokinetic Parameter Estimation

1994 ◽  
Vol 28 (4) ◽  
pp. 444-446
Author(s):  
Lawrence V. Friedrich ◽  
Roger L. White ◽  
Michael B. Kays ◽  
David S. Burgess
Keyword(s):  
Serum Concentration ◽  
Pharmacokinetic Parameter ◽  
Hplc Analysis ◽  
Statistical Significance ◽  
Pharmacokinetic Parameters ◽  
Parameter Estimates ◽  
Time Data ◽  
Drug Degradation ◽  
Concentration Time ◽  
The Impact

OBJECTIVE: To assess the impact of degradation of aztreonam, a beta-lactam antibiotic, during HPLC analysis on pharmacokinetic parameter estimates. METHODS: The baseline (B) serum concentration-time data from a published pharmacokinetic study of aztreonam were degraded using first-order equations and a degradation rate constant (0.014 h-1) determined from a preliminary degradation study. Samples were mathematically degraded for autosampler run times of 8–13 h (D1) to approximate a normal work day and for autosampler run times of 16–17 h (D2) and compared with B data. It was assumed that B data were nondegraded and that changes in chromatography were the result of degradation of azetreonam and not to any changes in chromatographic conditions. A two-compartment model was used to fit the data and pharmacokinetic parameters were calculated using standard equations. Statistical significance between all pharmacokinetic parameters for B and D1 and B and D2 was determined using the paired, two-tailed Student's t-test. RESULTS: Increased variability was noted for all pharmacokinetic parameters for D1 and D2 compared with B. Statistically significant differences were found for clearance (B <D1, p=0.0095 and B <D2, p=0.0194), steady-state volume of distribution (B <D2, p=0.0392), and area under the serum concentration-time curve (B >D1, p=0.0497). CONCLUSIONS: Aztreonam degradation resulted in increased variability in pharmacokinetic parameter estimates. Investigators should be cognizant of this and preliminary studies should be performed to characterize degradation for the length of the expected autosampler run.

RP-HPLC analysis of withanolides in the flowers, leaves, and roots ofWithania somnifera

2010 ◽  
Vol 22 (3) ◽  
pp. 473-480 ◽  
Author(s):  
N. W. Ali ◽  
S. Abouzid ◽  
A. Nasib ◽  
S. Khan ◽  
J. Qureshi ◽  
...  
Keyword(s):  
Hplc Analysis ◽  
Rp Hplc ◽  
Leaves And Roots

RP-HPLC ANALYSIS OF CARBONYL COMPOUNDS IN SOME INDOOR AIR SAMPLES IN A FACULTY FROM CLUJ-NAPOCA

2013 ◽  
Vol 12 (2) ◽  
pp. 219-225
Author(s):  
Carmen Roba ◽  
Cristina Rosu ◽  
Iulia Neamtiu ◽  
Eugen Gurzau
Keyword(s):  
Indoor Air ◽  
Carbonyl Compounds ◽  
Hplc Analysis ◽  
Air Samples ◽  
Rp Hplc

HPLC Analysis of Flavonoids from the Aerial Parts of Bupleurum Species

2010 ◽  
Vol 8 (2) ◽  
pp. 107-113 ◽  
Author(s):  
Ting-Ting ZHANG ◽  
Jin-Song ZHOU ◽  
Qiang WANG
Keyword(s):  
Hplc Analysis ◽  
Aerial Parts
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