Arabidopsis thaliana tryptophan synthase alpha: Gene cloning, expression, and subunit interaction

1995 ◽  
Vol 248 (6) ◽  
pp. 657-667 ◽  
Author(s):  
Elaine R. Radwanski ◽  
Jianmin Zhao ◽  
Robert L. Last
Keyword(s):  
Arabidopsis Thaliana ◽  
Gene Cloning ◽  
Tryptophan Synthase ◽  
Subunit Interaction ◽  
Alpha Gene

Characterization of tryptophan synthase alpha subunit mutants of Arabidopsis thaliana

1996 ◽  
Vol 253 (3) ◽  
pp. 353-361 ◽  
Author(s):  
E. R. Radwanski ◽  
A. J. Barczak ◽  
R. L. Last
Keyword(s):  
Arabidopsis Thaliana ◽  
Alpha Subunit ◽  
Tryptophan Synthase

Alpha-tryptophan synthase of Isatis tinctoria: gene cloning and expression

2008 ◽  
Vol 46 (7) ◽  
pp. 715-723 ◽  
Author(s):  
M. Salvini ◽  
T.M. Boccardi ◽  
E. Sani ◽  
R. Bernardi ◽  
S. Tozzi ◽  
...  
Keyword(s):  
Gene Cloning ◽  
Cloning And Expression ◽  
Gene Cloning And Expression ◽  
Tryptophan Synthase ◽  
Isatis Tinctoria

scholarly journals 5-Fluoroindole resistance identifies tryptophan synthase beta subunit mutants in Arabidopsis thaliana.

Genetics ◽  
1995 ◽  
Vol 140 (1) ◽  
pp. 303-313
Author(s):  
A J Barczak ◽  
J Zhao ◽  
K D Pruitt ◽  
R L Last
Keyword(s):  
Arabidopsis Thaliana ◽  
Protein A ◽  
Beta Subunit ◽  
Alpha Subunit ◽  
Anthranilate Synthase ◽  
Tryptophan Synthase ◽  
Wild Type ◽  
Recessive Mutations ◽  
The Stability

Abstract A study of the biochemical genetics of the Arabidopsis thaliana tryptophan synthase beta subunit was initiated by characterization of mutants resistant to the inhibitor 5-fluoroindole. Thirteen recessive mutations were recovered that are allelic to trp2-1, a mutation in the more highly expressed of duplicate tryptophan synthase beta subunit genes (TSB1). Ten of these mutations (trp2-2 through trp2-11) cause a tryptophan requirement (auxotrophs), whereas three (trp2-100 through trp2-102) remain tryptophan prototrophs. The mutations cause a variety of changes in tryptophan synthase beta expression. For example, two mutations (trp2-5 and trp2-8) cause dramatically reduced accumulation of TSB mRNA and immunologically detectable protein, whereas trp2-10 is associated with increased mRNA and protein. A correlation exists between the quantity of mutant beta and wild-type alpha subunit levels in the trp2 mutant plants, suggesting that the synthesis of these proteins is coordinated or that the quantity or structure of the beta subunit influences the stability of the alpha protein. The level of immunologically detectable anthranilate synthase alpha subunit protein is increased in the trp2 mutants, suggesting the possibility of regulation of anthranilate synthase levels in response to tryptophan limitation.

scholarly journals Subunit-subunit interaction of tryptophan synthase from hyperthermophile

2000 ◽  
Vol 40 (supplement) ◽  
pp. S118
Author(s):  
K. Ogasahara ◽  
M. Ishida ◽  
K. Yutani
Keyword(s):  
Tryptophan Synthase ◽  
Subunit Interaction

Mutations in the Contact Region between the α and β Subunits of Tryptophan Synthase Alter Subunit Interaction and Intersubunit Communication

Biochemistry ◽  
1998 ◽  
Vol 37 (9) ◽  
pp. 2961-2968 ◽  
Author(s):  
Roger Rowlett ◽  
Li-Hong Yang ◽  
S. Ashraf Ahmed ◽  
Peter McPhie ◽  
Kwang-Hwan Jhee ◽  
...  
Keyword(s):  
Contact Region ◽  
Tryptophan Synthase ◽  
Subunit Interaction ◽  
Α And Β Subunits ◽  
Intersubunit Communication
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