Complementation of the amylose-free starch mutant of potato (Solanum tuberosum.) by the gene encoding granule-bound starch synthase

1991 ◽  
Vol 82 (3) ◽  
pp. 289-295 ◽  
Author(s):  
E. R. van der Leij ◽  
R. G. E. Visser ◽  
K. Oosterhaven ◽  
D. A. M. van der Kop ◽  
E. Jacobsen ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Starch Synthase ◽  
Gene Encoding ◽  
Granule Bound Starch Synthase ◽  
Amylose Free

scholarly journals Expression of a cassava granule‐bound starch synthase gene in the amylose‐free potato only partially restores amylose content

1999 ◽  
Vol 22 (10) ◽  
pp. 1311-1318 ◽  
Author(s):  
Shah N. I. M. Salehuzzaman ◽  
Jean‐Paul Vincken ◽  
Marion Van De Wal ◽  
Evert Jacobsen ◽  
Richard G. F. Visser
Keyword(s):  
Amylose Content ◽  
Starch Synthase ◽  
Granule Bound Starch Synthase ◽  
Amylose Free

Cloning and Characterization of the Wx Gene Encoding a Granule-Bound Starch Synthase in Lotus (Nelumbo nucifera Gaertn)

2012 ◽  
Vol 30 (5) ◽  
pp. 1210-1217 ◽  
Author(s):  
Ye Lu ◽  
Liangjun Li ◽  
Yong Zhou ◽  
Qingsong Gao ◽  
Guohua Liang ◽  
...  
Keyword(s):  
Starch Synthase ◽  
Nelumbo Nucifera ◽  
Gene Encoding ◽  
Wx Gene ◽  
Granule Bound Starch Synthase

scholarly journals Pathogen and Pest Responses Are Altered Due to RNAi-Mediated Knockdown of GLYCOALKALOID METABOLISM 4 in Solanum tuberosum

2017 ◽  
Vol 30 (11) ◽  
pp. 876-885 ◽  
Author(s):  
Jamuna Risal Paudel ◽  
Charlotte Davidson ◽  
Jun Song ◽  
Itkin Maxim ◽  
Asaph Aharoni ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Biosynthetic Pathway ◽  
Plant Protection ◽  
Insect Pest ◽  
Metabolite Profile ◽  
Gene Encoding ◽  
Rnai Line ◽  
Leaf Consumption ◽  
Insect Mortality ◽  
Potato Growth

Steroidal glycoalkaloids (SGAs) are major secondary metabolites constitutively produced in cultivated potato Solanum tuberosum, and α-solanine and α-chaconine are the most abundant SGAs. SGAs are toxic to humans at high levels but their role in plant protection against pests and pathogens is yet to be established. In this study, levels of SGAs in potato were reduced by RNA interference (RNAi)-mediated silencing of GLYCOALKALOID METABOLISM 4 (GAME4)—a gene encoding cytochrome P450, involved in an oxidation step in the conversion of cholesterol to SGA aglycones. Two GAME4 RNAi lines, T8 and T9, were used to investigate the effects of manipulation of the SGA biosynthetic pathway in potato. Growth and development of an insect pest, Colorado potato beetle (CPB), were affected in these lines. While no effect on CPB leaf consumption or weight gain was observed, early instar larval death and accelerated development of the insect was found while feeding on leaves of GAME4 RNAi lines. Modulation of SGA biosynthetic pathway in GAME4 RNAi plants was associated with a larger alteration to the metabolite profile, including increased levels of one or both the steroidal saponins or phytoecdysteroids, which could affect insect mortality as well as development time. Colonization by Verticillium dahliae on GAME4 RNAi plants was also tested. There were increased pathogen levels in the T8 GAME4 RNAi line but not in the T9. Metabolite differences between T8 and T9 were found and may have contributed to differences in V. dahliae infection. Drought responses created by osmotic stress were not affected by modulation of SGA biosynthetic pathway in potato.

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