Isolation and identification of antialgal substances produced byPseudomonas aeruginosa

1993 ◽  
Vol 5 (3) ◽  
pp. 297-306 ◽  
Author(s):  
A. Dakhama ◽  
J. de la Noüe ◽  
M. C. Lavoie
Keyword(s):  
Isolation And Identification ◽  
Antialgal Substances

Growth Inhibition of Two Typical Red Tide Microalgae (Karenia mikimitoi and Alexandrium tamarense) by Antialgal Substances from Gracilaria lemaneiformis and its Isolation and Identification

2012 ◽  
Vol 610-613 ◽  
pp. 3-8
Author(s):  
Ying Ying Sun ◽  
Shen Zhen Xu ◽  
Jing Zhang ◽  
Bin Lun Yan
Keyword(s):  
Growth Inhibition ◽  
Red Tide ◽  
Inhibitory Effect ◽  
Alexandrium Tamarense ◽  
Gracilaria Lemaneiformis ◽  
Isolation And Identification ◽  
Liquid Liquid Extraction ◽  
Red Tide Microalgae ◽  
Antialgal Substances ◽  
Significant Growth Inhibition

A yellow-brown extract (GLME) was obtained by methanol extraction of powder from Gracilaria lemaneiformis. The methanol extract was further fractionated by liquid-liquid extraction and obtained the four fractions (F-Ⅰ, F-Ⅱ, F-Ⅲ and F-Ⅳ). Growth inhibition of two typical red tide microalgae (Karenia mikimitoi and Alexandrium tamarense) by those fractions and its isolated fractions were investigated. The results showed that crude extract had inhibitory activity against the growth of Karenia mikimitoi and Alexandrium tamarense. Among the four fractions, F-Ⅱ exhibited the strongest effect against the growth of the two red tide microalgae, and the inhibitory effect of Karenia mikimitoi and Alexandrium tamarense by F-Ⅱ at the concentration of 0.35 g/L was 48.8% and 46.7% in day 12, respectively. After additional isolation of F-II with five organic solvents (petroleum ether, petroleum ether:ethyl acetate=1:1, ethyl acetate, ethyl acetate:acetone=1:1 and acetone), the F- II was separated into five fractions of Ⅱ-A, Ⅱ-B, Ⅱ-C, Ⅱ-D and Ⅱ-E on silicon gel column, respectively. The eluant Ⅱ-B andⅡ-C showed the significant growth inhibition activities. The antialgal substances in eluant Ⅱ-B andⅡ-C are identified by comprehensive analysis of UV and HPLC-MS to be chlorogenic acid, hydroxybenzoic acid and vanillic acid. And they significantly inhibited the growth of Karenia mikimitoi and Alexandrium tamarense.

Isolation and identification of chemical constituents from the roots of Peucedanum praeruptorum

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
YJ Lee ◽  
J Kim ◽  
J Lee ◽  
ES Cho ◽  
OS Bang
Keyword(s):  
Chemical Constituents ◽  
Isolation And Identification

Isolation and identification of neuroprotective compounds from Machilus thunbergii

Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
CJ Ma ◽  
KY Lee ◽  
HK Yang ◽  
JS Yoon ◽  
YC Kim ◽  
...  
Keyword(s):  
Isolation And Identification ◽  
Machilus Thunbergii

Isolation of post operative hospital acquired infections in a private hospital of Bangladesh and identification of clonal relatedness of E. coli by using Pulse filed gel electrophoresis

2012 ◽  
Vol 6 (2) ◽  
pp. 7-10
Author(s):  
Mohammad Murshed ◽  
Sabeena Shahnaz ◽  
Md. Abdul Malek
Keyword(s):  
Private Hospital ◽  
Isolation And Identification ◽  
College Hospital ◽  
Hospital Acquired Infection ◽  
E Coli ◽  
Hospital Acquired Infections ◽  
Medical College ◽  
Clonal Relatedness ◽  
Hospital Acquired ◽  
Dressing Materials

Isolation and identification of post operative hospital acquired infection was carried out from July 2008 to December 2008 in Holy Family Red Crescent Medical College Hospital (private hospital). The major pathogen of wound infection was E. coli. A total; of 120 samples were collected from the surrounding environment of post operative room like floor, bed sheets, instruments, dressing materials, catheter, nasogastric and endotracheal tube. E. coli (40%) was the predominant organism followed by S. aureus (24%). DNA fingerprinting analysis using pulsed field gel electreopheresis of XbaI restriction digested genomic DNA showed that clonal relatedness between the two clinical nd environmental isolates were 100%.DOI: http://dx.doi.org/10.3329/bjmm.v6i2.19369 Bangladesh J Med Microbiol 2012; 06(02): 7-10

Investigation of duck plague virus in hoar areas of Bangladesh

2020 ◽  
Vol 26 (1-2) ◽  
pp. 73-78
Author(s):  
A Hossen ◽  
MH Rahman ◽  
MZ Ali ◽  
MA Yousuf ◽  
MZ Hassan ◽  
...  
Keyword(s):  
Cytopathic Effect ◽  
Clinical Sample ◽  
Chorioallantoic Membrane ◽  
Clinical Samples ◽  
Isolation And Identification ◽  
Duck Plague Virus ◽  
Pcr Method ◽  
Polymerase Chain ◽  
The Individual ◽  
Free Ranging

Duck plague (DP) is the most important infectious disease of geese, ducks and free-ranging water birds. The present study was conducted to determine the prevalence of duck plague virus followed by isolation and identification. For these purposes, a total of 155 cloacal swabs samples were collected randomly from duck of different haor areas of Bangladesh including 45 (41 surveillance and 4 clinical) samples from Netrokona; 42 (40 surveillance and 2 clinical) samples from Kishoregonj; 30 samples from Brahmanbaria and 38 samples from Sunamganj. The samples were processed and pooled (1:5 ratio) for initial screening of target polymerase gene of duck plague virus by polymerase chain reaction (PCR) method. All the samples of a positive pool were then tested individually for identifying the individual positive samples. The result showed that out of 155 samples, 41 (26.45%) were found positive in which 17 were from Netrokona, where 15 (36.58%) were from surveillance samples and 2 (50%) were from clinical sample; 16 were from Kishoregonj, where 14 (35%) were from surveillance samples and 2 (100%) were from clinical sample; 2 (6.6%) were from Brahmanbaria and 5 (13.15%) were from Sunamganj. These positive samples were inoculated into 9-10 days embryonated duck eggs (EDE) through chorioallantoic membrane (CAM) route for the isolation of virus. The EDE died earlier was also chilled, and in a similar way, the CAMs were collected and again performed PCR for id entification of virus. Out of 41 PCR positive samples, 26 samples were isolated and reconfirmed by PCR. Subsequently, DPV was isolated in primary duck embryo fibroblasts cell culture and confirmed by observing cytopathic effect (CPE). Bang. J. Livs. Res. Vol. 26 (1&2), 2019: P. 73-78

Isolation and identification of plant growth-promoting bacteria associated with tall fescue

2009 ◽  
pp. 211-216 ◽  
Author(s):  
J. Monk ◽  
E. Gerard ◽  
S. Young ◽  
K. Widdup ◽  
M. O'Callaghan
Keyword(s):  
New Zealand ◽  
Plant Growth ◽  
Tall Fescue ◽  
Festuca Arundinacea ◽  
Plant Growth Promoting Bacteria ◽  
Beneficial Bacteria ◽  
Isolation And Identification ◽  
Naturally Occurring ◽  
Plant Growth Promoting ◽  
Growth Promoting

Tall fescue (Festuca arundinacea) is a useful alternative to ryegrass in New Zealand pasture but it is slow to establish. Naturally occurring beneficial bacteria in the rhizosphere can improve plant growth and health through a variety of direct and indirect mechanisms. Keywords: rhizosphere, endorhiza, auxin, siderophore, P-solubilisation

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