scholarly journals Erratum to: Lipids of epiphyseal cartilage

1962 ◽  
Vol 18 (10) ◽  
pp. 480-480
Author(s):  
V. Zambotti ◽  
I. Cescon ◽  
B. Bonferroni ◽  
L. Bolognani
Keyword(s):  
Author(s):  
H. Clarke Anderson ◽  
Priscilla R. Coulter

Epiphyseal cartilage matrix contains fibrils and particles of at least 5 different types: 1. Banded collagen fibrils, present throughout the matrix, but not seen in the lacunae. 2. Non-periodic fine fibrils <100Å in diameter (Fig. 1), which are most notable in the lacunae, and may represent immature collagen. 3. Electron dense matrix granules (Fig. 1) which are often attached to fine fibrils and collagen fibrils, and probably contain protein-polysaccharide although the possibility of a mineral content has not been excluded. 4. Matrix vesicles (Fig. 2) which show a selective distribution throughout the epiphysis, and may play a role in calcification. 5. Needle-like apatite crystals (Fig. 2).Blocks of formalin-fixed epiphysis from weanling mice were digested with the following agents in 0.1M phosphate buffer: a) 5% ethylenediaminetetraacetate (EDTA) at pH 8.3, b) 0.015% bovine testicular hyaluronidase (Sigma, type IV, 750 units/mg) at pH 5.5, and c) 0.1% collagenase (Worthington, chromatograhically pure, 200 units/mg) at pH 7.4. All digestions were carried out at 37°C overnight. Following digestion tissues were examined by light and electron microscopy to determine changes in the various fibrils and particles of the matrix.


2014 ◽  
Vol 32 (5) ◽  
pp. 661-668 ◽  
Author(s):  
Maria Elena Candela ◽  
Leslie Cantley ◽  
Rika Yasuaha ◽  
Masahiro Iwamoto ◽  
Maurizio Pacifici ◽  
...  

1956 ◽  
Vol 93 (2) ◽  
pp. 382-384 ◽  
Author(s):  
R. H. Follis ◽  
P. L. Melanotte

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