TheCBP2 gene fromSaccharomyces douglasii is a functional homologue of theSaccharomyces cerevisiae gene and is essential for respiratory growth in the presence of a wild-type (intron-containing) mitochondrial genome

1996 ◽  
Vol 250 (3) ◽  
pp. 316-322 ◽  
Author(s):  
G-Y. Li ◽  
G-L. Tian ◽  
P. P. Slonimski ◽  
C. J. Herbert
Keyword(s):  
Mitochondrial Genome ◽  
Wild Type ◽  
Respiratory Growth

The mitochondrial genome of wild-type yeast cells

1978 ◽  
Vol 119 (2) ◽  
pp. 213-235 ◽  
Author(s):  
Godeleine Fonty ◽  
Regina Goursot ◽  
David Wilkie ◽  
Giorgio Bernardi
Keyword(s):  
Mitochondrial Genome ◽  
Yeast Cells ◽  
Wild Type ◽  
Wild Type Yeast

The mitochondrial genome of wild-type yeast cells

1972 ◽  
Vol 65 (2) ◽  
pp. 207-212 ◽  
Author(s):  
Stanslav D. Ehrlich ◽  
Jean-Paul Thiery ◽  
Giorgio Bernardi
Keyword(s):  
Mitochondrial Genome ◽  
Yeast Cells ◽  
Wild Type ◽  
Wild Type Yeast

A Podospora anserina longevity mutant with a temperature-sensitive phenotype for senescence

1987 ◽  
Vol 7 (9) ◽  
pp. 3199-3204
Author(s):  
M S Turker ◽  
J G Nelson ◽  
D J Cummings
Keyword(s):  
Mitochondrial Genome ◽  
Type Strain ◽  
Wild Type Strain ◽  
Podospora Anserina ◽  
Specific Region ◽  
Temperature Sensitive ◽  
Wild Type ◽  
Temperature Sensitive Phenotype

A Podospora anserina longevity mutant was identified with a temperature-sensitive phenotype for senescence. This mutant, termed TS1, grew for over 3 m at 27 degrees C, but when shifted to 34 degrees C, it underwent senescence between 10 and 18 cm. A previously described senescence-associated plasmid, alpha senDNA, derived from the mitochondrial genome, was not detected in TS1 at 27 degrees C but was present in senescent cultures at 34 degrees C. A similar result was observed in progeny strains obtained by crossing the TS1 mutant with a wild-type strain. Other mitochondrial excision-amplification DNAs in addition to alpha senDNA were also observed in the senescent cultures. Most were derived from a specific region of the mitochondrial genome. These results provide evidence that alpha senDNA is involved in TS1 senescence and suggest that this plasmid may play a role in the formation of other mitochondrial excision-amplification plasmids.

The mitochondrial genome of wild-type yeast cells

1972 ◽  
Vol 65 (2) ◽  
pp. 173-189 ◽  
Author(s):  
Giorgio Bernardi ◽  
Gianni Piperno ◽  
Godeleine Fonty
Keyword(s):  
Mitochondrial Genome ◽  
Yeast Cells ◽  
Wild Type ◽  
Wild Type Yeast

The mitochondrial genome of wild-type yeast cells

1977 ◽  
Vol 110 (1) ◽  
pp. 17-47 ◽  
Author(s):  
Ariel Prunell ◽  
Helena Kopecka ◽  
François Strauss ◽  
Giorgio Bernardi
Keyword(s):  
Mitochondrial Genome ◽  
Yeast Cells ◽  
Wild Type ◽  
Wild Type Yeast

T7 RNA polymerase-dependent expression of COXII in yeast mitochondria

1994 ◽  
Vol 14 (7) ◽  
pp. 4643-4652
Author(s):  
J L Pinkham ◽  
A M Dudley ◽  
T L Mason
Keyword(s):  
Mitochondrial Genome ◽  
Rna Polymerase ◽  
Target Gene ◽  
Expression System ◽  
T7 Rna Polymerase ◽  
Cellular Respiration ◽  
Detectable Effect ◽  
Wild Type ◽  
Cox2 Expression ◽  
Adh1 Promoter

An in vivo expression system has been developed for controlling the transcription of individual genes in the mitochondrial genome of Saccharomyces cerevisiae. The bacteriophage T7 RNA polymerase (T7Pol), fused to the COXIV mitchondrial import peptide and expressed under the control of either the GAL1 or the ADH1 promoter, efficiently transcribes a target gene, T7-COX2, in the mitochondrial genome. Cells bearing the T7-COX2 gene, but lacking wild-type COX2, require T7Pol for respiration. Functional expression of T7-COX2 is completely dependent on the COX2-specific translational activator Pet111p, despite additional nucleotides at the 5' end of the T7-COX2 transcript. Expression of mitochondrion-targeted T7Pol at high levels from the GAL1 promoter has no detectable effect on mitochondrial function in rho+ cells lacking the T7-COX2 target gene, but in cells with T7-COX2 integrated into the mitochondrial genome, an equivalent level of T7Pol expression causes severe respiratory deficiency. In comparison with wild-type COX2 expression, steady-state levels of T7-COX2 mRNA increase fivefold when transcription is driven by T7Pol expressed from the ADH1 promoter, yet COXII protein levels and cellular respiration rates decrease by about 50%. This discoordinate expression of mRNA and protein provides additional evidence for posttranscriptional control of COX2 expression.

scholarly journals Oxygen and haem regulate the synthesis of peroxisomal proteins: catalase A, acyl-CoA oxidase and Pex1p in the yeast Saccharomyces cerevisiae; the regulation of these proteins by oxygen is not mediated by haem

2000 ◽  
Vol 350 (1) ◽  
pp. 313-319 ◽  
Author(s):  
Marek SKONECZNY ◽  
Joanna RYTKA
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mitochondrial Genome ◽  
Oxygen Sensor ◽  
Transcriptional Factor ◽  
The Other ◽  
Wild Type ◽  
Yeast Saccharomyces Cerevisiae ◽  
Regulatory Factors ◽  
Genes Encoding ◽  
Acyl Coa Oxidase

Saccharomyces cerevisiae genes related to respiration are typically controlled by oxygen and haem. Usually the regulation by these factors is co-ordinated; haem is indicated as the oxygen sensor. However, the responsiveness of peroxisome functions to these regulatory factors is poorly understood. The expression of CTA1, POX1 and PEX1 genes encoding the peroxisomal proteins catalase A, acyl-CoA oxidase and Pex1p peroxin respectively was studied under various conditions: in anaerobiosis, in the absence of haem and in respiratory incompetence caused by the lack of a mitochondrial genome (ρ0). The influence of haem deficiency or ρ0 on peroxisomal morphology was also investigated. Respiratory incompetence has no effect on the expression of CTA1 and POX1, whereas in the absence of haem their expression is markedly decreased. The synthesis of Pex1p is decreased in ρ0 cells and is decreased even more in haem-deficient cells. Nevertheless, peroxisomal morphology in both these types of cell does not differ significantly from the morphology of peroxisomes in wild-type cells. The down-regulating effect of anoxia on the expression of CTA1 and POX1 is even stronger than the effect of haem deficiency and is not reversed by the addition of exogenous haem or the presence of endogenous haem. Moreover, neither of these genes responds to the known haem-controlled transcriptional factor Hap1p. In contrast with the other two genes studied, PEX1 is up-regulated in anaerobiosis. The existence of one or more novel mechanisms of regulation of peroxisomal genes by haem and oxygen, different from those already known in S. cerevisiae, is postulated.

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