Separation and identification of the degradation products of purines and nucleic acids on acid hydrolysis by circular paper chromatography

1953 ◽  
Vol 9 (8) ◽  
pp. 296-297 ◽  
Author(s):  
K. V. Giri ◽  
P. R. Krishnaswamy ◽  
G. D. Kalyankar ◽  
P. L. Narasimha Rao
Keyword(s):  
Nucleic Acids ◽  
Acid Hydrolysis ◽  
Paper Chromatography ◽  
Degradation Products ◽  
Circular Paper ◽  
Circular Paper Chromatography

STUDIES ON ASPERGILLI X.: UTILIZATION OF POLYSACCHARIDES

1963 ◽  
Vol 9 (5) ◽  
pp. 703-707 ◽  
Author(s):  
V. P. Agnihotri
Keyword(s):  
Paper Chromatography ◽  
Hydrolytic Product ◽  
Circular Paper ◽  
Circular Paper Chromatography

The utilization of starch, dextrin, inulin, and glycogen by A. clavatus Desmazieres, A. sclerotiorum Huber, A. indicus Mehrotra and Agnihotri, and A. allahabadii Mehrotra and Agnihotri was studied through circular paper chromatography. These polysaccharides were evidently assimilated through transglycosidation. It was noticed that these fungi, during the process of utilization of these polysaccharides, produced some additional oligosaccharides some of which could not be identified.All four molds showed better growth on starch, dextrin, and glycogen than on glucose (final hydrolytic product of these polysaccharides); however, these organisms showed better growth on fructose than on inulin.

THE ESTIMATION OF NUCLEIC ACIDS IN SOME ALGAE AND HIGHER PLANTS

1960 ◽  
Vol 38 (1) ◽  
pp. 31-49 ◽  
Author(s):  
Robert M. Smillie ◽  
G. Krotkov
Keyword(s):  
Nucleic Acids ◽  
Paper Chromatography ◽  
Exchange Resin ◽  
Higher Plants ◽  
Anion Exchange Resin ◽  
Degradation Products ◽  
Higher Plant ◽  
High Acid Concentration ◽  
Wide Range ◽  
Quantitative Separation

Several current methods for the extraction and estimation of nucleic acids in biological materials were applied to Euglena and other plants. The efficiency of both the preliminary extractions for acid-soluble-P and lipid-P and the subsequent extraction of the nucleic acids was studied. A relatively high acid concentration (15% TCA) was required to directly extract all the acid-soluble phosphates. These conditions appeared to remove a small amount of the RNA. Lower acid concentrations as used in the Ogur–Rosen method (2% PCA) failed to extract all the acid-soluble phosphates. By using a modification of the Ogur–Rosen initial extraction method, the acid-soluble phosphates were quantitatively extracted without loss of RNA. After removal of the acid-soluble phosphates and lipid phosphates, the plant nucleic acids were quantitatively extracted by either the Schmidt–Thannhauser or Schneider methods. In many of the plants tested, the presence of pentose-containing polysaccharides, protein degradation products, or polyphosphate (algae only) interfered in estimations based on either the Schneider or Schmidt–Thannhauser procedures. Such interfering substances in the Schmidt–Thannhauser method were eliminated by the use of an anion exchange resin. Details are given of a modified Schmidt–Thannhauser procedure which should be suitable for a wide range of plants. The modified procedure may be simplified for Euglena and some higher plant tissues depending on the nature and quantities of interfering substances present. Methods are also given for the quantitative separation of plant RNA nucleotides by paper chromatography and by ion exchange paper chromatography.

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