Neural differentiation in the OTT-6050 mouse teratoma

1981 ◽  
Vol 392 (3) ◽  
pp. 281-294 ◽  
Author(s):  
Scott R. VandenBerg ◽  
Marcel Chatel ◽  
Owen M. Griffiths ◽  
Stephen J. DeArmond ◽  
Conrad Pappas ◽  
...  
Keyword(s):  
Neural Differentiation ◽  
Mouse Teratoma

Neural differentiation in the OTT-6050 mouse teratoma

1981 ◽  
Vol 392 (3) ◽  
pp. 295-308 ◽  
Author(s):  
Scott R. VandenBerg ◽  
J. Ronald Hess ◽  
Mary M. Herman ◽  
Stephen J. DeArmond ◽  
Meredith Halks-Miller ◽  
...  
Keyword(s):  
Neural Differentiation ◽  
Mouse Teratoma

Neural differentiation in the OTT-6050 mouse teratoma: Enzymatic and immunofluorescence characterization of a tumor fraction showing melanogenesis in neuroepithelial cells

1981 ◽  
Vol 393 (1) ◽  
pp. 27-37 ◽  
Author(s):  
Elizabeth Erdelyi ◽  
Scott R. VandenBerg ◽  
J. Raese ◽  
Jack D. Barchas ◽  
Lucien J. Rubinstein ◽  
...  
Keyword(s):  
Neural Differentiation ◽  
Neuroepithelial Cells ◽  
Mouse Teratoma

scholarly journals Ghrelin peptide improves glial conditioned medium effects on neuronal differentiation of human adipose mesenchymal stem cells

2021 ◽  
Author(s):  
Cristina Russo ◽  
Giuliana Mannino ◽  
Martina Patanè ◽  
Nunziatina Laura Parrinello ◽  
Rosalia Pellitteri ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Conditioned Medium ◽  
Neural Differentiation ◽  
Conditioned Media ◽  
Glial Fibrillary Acid Protein ◽  
Ghrelin Receptor ◽  
Neuronal Markers ◽  
Adipose Mesenchymal Stem Cells ◽  
Marker Expression

AbstractThe influences of ghrelin on neural differentiation of adipose-derived mesenchymal stem cells (ASCs) were investigated in this study. The expression of typical neuronal markers, such as protein gene product 9.5 (PGP9.5) and Microtubule Associated Protein 2 (MAP2), as well as glial Fibrillary Acid Protein (GFAP) as a glial marker was evaluated in ASCs in different conditions. In particular, 2 µM ghrelin was added to control ASCs and to ASCs undergoing neural differentiation. For this purpose, ASCs were cultured in Conditioned Media obtained from Olfactory Ensheathing cells (OEC-CM) or from Schwann cells (SC-CM). Data on marker expression were gathered after 1 and 7 days of culture by fluorescence immunocytochemistry and flow cytometry. Results show that only weak effects were induced by the addition of only ghrelin. Instead, dynamic ghrelin-induced modifications were detected on the increased marker expression elicited by glial conditioned media. In fact, the combination of ghrelin and conditioned media consistently induced a further increase of PGP9.5 and MAP2 expression, especially after 7 days of treatment. The combination of ghrelin with SC-CM produced the most evident effects. Weak or no modifications were found on conditioned medium-induced GFAP increases. Observations on the ghrelin receptor indicate that its expression in control ASCs, virtually unchanged by the addition of only ghrelin, was considerably increased by CM treatment. These increases were enhanced by combining ghrelin and CM treatment, especially at 7 days. Overall, it can be assumed that ghrelin favors a neuronal rather than a glial ASC differentiation.

scholarly journals Simultaneous measurement of neurite and neural body mass accumulation via quantitative phase imaging

The Analyst ◽  
2021 ◽  
Author(s):  
Soorya Pradeep ◽  
Tasmia Tasnim ◽  
Huanan Zhang ◽  
Thomas A. Zangle
Keyword(s):  
Body Mass ◽  
Biomass Production ◽  
Simultaneous Measurement ◽  
Neural Differentiation ◽  
Phase Imaging ◽  
Quantitative Phase Imaging ◽  
Quantitative Phase ◽  
Mass Accumulation

Quantitative phase imaging (QPI) used to quantify the mass of soma (cell bodies) and neurites as well as the rates of biomass production due to neurite maturation and formation during neural differentiation.

Sign in / Sign up

Export Citation Format

Share Document