Determination of the dry mass of nervous and glial cell nuclei by interference microscopy

1965 ◽  
Vol 21 (6) ◽  
pp. 320-321 ◽  
Author(s):  
Maria Pia Viola ◽  
E. Puccinelli
Keyword(s):  
Glial Cell ◽  
Dry Mass ◽  
Interference Microscopy ◽  
Cell Nuclei

scholarly journals I. QUANTITATIVE DETERMINATION OF THE AMOUNT OF DNA PER NUCLEUS BY INTERFERENCE MICROSCOPY

1969 ◽  
Vol 42 (2) ◽  
pp. 444-451 ◽  
Author(s):  
Cecilia Bibbiani ◽  
Roberto Tongiani ◽  
Maria Pia Viola-Magni
Keyword(s):  
Quantitative Determination ◽  
Dry Mass ◽  
Interference Microscopy ◽  
Isolated Nuclei ◽  
Adult Rat ◽  
Dnase Treatment ◽  
Dna Values ◽  
Before And After ◽  
Dna Complex

A method for the determination of the DNA content of isolated nuclei of different ploidy has been developed. It is based on measurement of the nuclear dry mass, with an integrating microinterferometer, before and after DNase treatment. The values found are slightly low, because, as indicated by biochemical determinations, consistently 5% to 8% of DNA is not extracted by DNase under these conditions. The average DNA values thus obtained for diploid and tetraploid nuclei of adult rat liver are 7.7 and 15.6 pg (10-12 g), respectively. Definite advantages of this procedure are: i) comparisons with biochemical determinations to give DNA values for each class of ploidy, ii) comparisons with histophotometry of the Feulgen dye-DNA complex to give absolute values instead of arbitrary units.

scholarly journals Evaluation of Usefulness of the Designed Laboratory Photobioreactor for Microalgae Cultivation in Controlled Conditions

2016 ◽  
Vol 20 (1) ◽  
pp. 13-22
Author(s):  
Beata Brzychczyk ◽  
Zbigniew Kowalczyk ◽  
Jan Giełżecki
Keyword(s):  
Culture Medium ◽  
Dry Mass ◽  
Biomass Growth ◽  
Microalgae Cultivation ◽  
Freshwater Microalgae ◽  
Laboratory Conditions ◽  
Medium Concentration ◽  
Controlled Conditions ◽  
The Impact

AbstractThe objective of the paper was to analyse the use of the designed photobioreactor for freshwater microalgae cultivation in the controlled laboratory conditions. The work covered the design and construction of photobioreactors (PBR) and setting up comparative cultivations of freshwater microalgae chlorelli vulgaris along with determination of the biomass growth intensity for a varied amount of supplied culture medium. It was found out that the constructed PBR may be used for microalgae cultivation in the controlled conditions. The impact of the culture medium amount on the growth of chlorelli vulgaris was proved. As a result of the increase of culture medium concentration to 30.1-120.4 ml·l−1 of water, dry mass in photobioreactorsincreased respectively from 1.33 g·dm−3 to 4.68 g·dm−3.

Microradiographical Determination of the Dry Mass Density in the Rat Retina1

2009 ◽  
Vol 71 (S208) ◽  
pp. 33c-39c
Author(s):  
Enping Chen ◽  
Jang-Hyun Chung ◽  
Per G. Söderberg ◽  
Bo Lindström
Keyword(s):  
Mass Density ◽  
Dry Mass

scholarly journals Unequal Distribution of ATP: NMN Adenylyltransferase Activity among Neuronal and Glial Cell Nuclei

1966 ◽  
Vol 42 (10) ◽  
pp. 1217-1222 ◽  
Author(s):  
Masanori KUROKAWA ◽  
Takahiko KATO ◽  
Hiroshi INAMURA
Keyword(s):  
Glial Cell ◽  
Cell Nuclei ◽  
Unequal Distribution

scholarly journals Effect of temperature on dry mass of polytene nuclei in Drosophila

1979 ◽  
Vol 38 (1) ◽  
pp. 405-416
Author(s):  
I.J. Hartmann-Goldstein ◽  
D.J. Goldstein
Keyword(s):  
Salivary Glands ◽  
Dry Mass ◽  
Nuclear Volume ◽  
Interference Microscopy ◽  
Effect Of Temperature ◽  
Percentage Loss ◽  
Mass Group ◽  
The Mean ◽  
Polytene Nuclei ◽  
Aqueous Detergent

Nuclei were isolated by an aqueous detergent method from Drosophila prepupal salivary glands, and measured by integrating interference microscopy. There was a highly significant correlation between nuclear volume and dry mass. Dry masses fell into 2, 3 or 4 distinct groups corresponding to polytene replication classes; the mean of a given dry mass group was between 8 and 30% less than twice that of the group below, indicating that the ratio of DNA:dry mass increases during polytenic growth. The proportion of nuclei in the higher mass groups, the mean dry mass of nuclei within a given mass group, and the percentage loss of nuclear dry mass in the first hour after isolation were all higher when animals were reared at 15 degrees instead of 25 degrees C. Nuclear dry mass in prepupae was affected by the temperature during both the embryonic and larval periods, and also to some extent by the nutrition and degree of crowding of the cultures.

scholarly journals Effects of plant density and proportion on the interaction between wheat with alexandergrass plants

Bragantia ◽  
2011 ◽  
Vol 70 (1) ◽  
pp. 40-45 ◽  
Author(s):  
Leonardo Bianco de Carvalho ◽  
Pedro Luis da Costa Aguiar Alves ◽  
José Valcir Fidelis Martins
Keyword(s):  
Growth And Development ◽  
Plant Density ◽  
Block Design ◽  
Dry Mass ◽  
Wheat Crop ◽  
Replacement Series ◽  
Mixture Experiment ◽  
Randomized Complete Block Design ◽  
Production Characteristics

Determination of competitive relationships among plant species requires appropriate experimental designs and method of analysis. The hypothesis of this research was that two species growing in coexistence show different growth and development due to their relative competitiveness. This research aims to measure the relative competitiveness of wheat crop compared to Alexandergrass by the interpretation of plant density and proportional effects using replacement series experiments. Monocultures were cultivated in densities of 1, 3, 5, 10 and 15 plants per pot and analyzed by regression of dry mass data. Mixture experiment was cultivated in wheat:Alexandergrass proportions of 0:6, 1:5, 2:4, 3:3, 4:2, 5:1 and 6:0 plants per pot and analyzed by graphical interpretation of growth and production characteristics. Both experiments were carried out in randomized complete block design with four replicates. Alexandergrass was more sensitive to intraspecific competition than wheat. Alexandergrass was lightly more competitive than wheat. Number and weight of spikes and number of tillers were the wheat characteristics more affected by Alexandergrass interference.

scholarly journals Phosphorus fertilization and growth of buffel grass (Cenchrus ciliares L.) cultivars

2015 ◽  
Vol 19 (1) ◽  
pp. 34-38
Author(s):  
Vanderlise Giongo ◽  
Alessandra M. Salviano ◽  
Betina R. C. dos Santos ◽  
Emylly F. Leal
Keyword(s):  
Critical Level ◽  
Dry Mass ◽  
Phosphorus Fertilization ◽  
Critical Levels ◽  
Buffel Grass ◽  
P Accumulation ◽  
P Content ◽  
Phosphorus Requirement ◽  
Set Up

Phosphorus (P) plays an important role in the growth of root system as well as the tillering grass, being fundamental to increase the productivity of these species. The aim of this study was to evaluate the development of buffel grass cultivars and establish critical values of P in plant and soil. The experimental set up was a 4 x 5 factorial scheme (four Cenchrus ciliaris cvs.: Biloela, Aridus, CPATSA 7754 and Pusa Giant, and five doses of P2O5 - 0, 30, 60, 90 and 120 kg ha-1) with four replications. After 90 days of cultivation, dry mass of shoot (DMS) and root (DMR) production and the P accumulation (Pacc) were determined. Soil samples to determine the P content and determination of the critical level (CriLev) were also collected. The cv. Biloela presented lower DMR and DMS production compared to the other cultivars. The cultivares Biloela, Pusa Giant and Aridus showed different critical levels of P in soil and plant, obtained in the greenhouse showing that they have different requirement of this nutrient for their growth. The cultivar CPATSA 7754 showed higher phosphorus requirement and did not permit to establish critical levels with doses used in the present study.

scholarly journals Innovative application of the moisture analyzer for determination of dry mass content of processed cheese

2018 ◽  
Vol 54 (10) ◽  
pp. 3071-3080 ◽  
Author(s):  
Małgorzata Kowalska ◽  
Sławomir Janas ◽  
Magdalena Woźniak
Keyword(s):  
Dry Mass ◽  
Mass Content ◽  
Processed Cheese
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