Hydrolysis of amino acidβ-naphthylamides by aminopeptidases in human parotid saliva and human serum

1968 ◽  
Vol 24 (4) ◽  
pp. 347-348 ◽  
Author(s):  
I. Nagatsu ◽  
T. Nagatsu ◽  
T. Yamamoto
Keyword(s):  
Human Serum ◽  
Parotid Saliva ◽  
Hydrolysis Of ◽  
Human Parotid Saliva

scholarly journals The binding of calcium to a salivary phosphoprotein, protein A, common to human parotid and submandibular secretions

1976 ◽  
Vol 155 (1) ◽  
pp. 163-169 ◽  
Author(s):  
A Bennick
Keyword(s):  
Calcium Binding ◽  
Protein C ◽  
Equilibrium Dialysis ◽  
Protein A ◽  
Side Chain ◽  
Carboxyl Groups ◽  
Parotid Saliva ◽  
Submandibular Saliva ◽  
Hydrolysis Of ◽  
Human Parotid Saliva

The binding of Ca2+ to a previously described phosphoprotein from human parotid saliva, protein A [Bennick (1975) Biochem J. 145, 557-567] was studied by means of equilibrium dialysis. In 5 mM-Tris/HC1 buffer, pH7.5, protein A bound 664nmol of Ca/mg of protein. Km was determined to be 181 muM and the binding of Ca2+ to the protein was non-co-operative. The binding of Ca2+ apparently occurs to side-chain carboxyl groups in the protein, but protein phosphate is of minor if any importance in calcium binding. Hydrolysis of protein A by trypsin and collagenase or heating of the protein at 60 degrees or 100 degrees C did not affect Ca2+ binding. The Ca2+ binding decreases with increased concentration of the dialysis buffer and on the addition of SrCl2, or MgCl2 or MnCl2 to the dialysis buffer. Protein A does not aggregate in the presence of Ca2+, since the s20,w was identical when determined in the presence (1.30S) and absence (1.35S) of CaCl2. By use of a specific antiserum to protein A it was found that protein C [Bennick & Connell (1971) Biochem. J. 123, 455-464] and perhaps minor related components cross-reacted with protein A. No other salivary proteins showed immunological similarity. Proteins A and C were also present in submandibular saliva. The possible functions of protein A are discussed.

Hydrolysis of Glycol Chitin by an Endo- and Exo-Type of β-N-Acetylglucosaminidase from Human Parotid Saliva

1973 ◽  
Vol 52 (4) ◽  
pp. 846-846 ◽  
Author(s):  
Tatsuo Watanabe ◽  
Yoshifumi Iwamoto ◽  
Akira Tsunemitsu
Keyword(s):  
Parotid Saliva ◽  
Hydrolysis Of ◽  
Human Parotid Saliva

Synthesis of conjugates of 1-(carboxymethyl)cytosine and 1-(5-O-carboxymethyl-β-D-arabinofuranosyl)cytosine with proteins

1979 ◽  
Vol 44 (10) ◽  
pp. 3023-3032 ◽  
Author(s):  
Helmut Pischel ◽  
Antonín Holý ◽  
Günther Wagner
Keyword(s):  
Human Serum Albumin ◽  
Acetic Anhydride ◽  
Serum Albumin ◽  
Human Serum ◽  
Activated Esters ◽  
Hydrolysis Of

1-(Carboxymethyl)cytosine (Ia), 1-(5-O-carboxymethyl-β-D-arabinofuranosyl)cytosine (IIa) and 5'-O-carboxylmethylcytidine (IIIa) were transformed by treatment with acetic anhydride and 4-dimethylaminopyridine to the peracetyl derivatives Ib-IIIb. These products reacted with p-nitrophenol in the presence of N, N'-dicyclohexylcarbodiimide to give the activated esters Ic-IIIc which on reaction with ammonia, dimethylamine or 2-aminoethanol afforded the corresponding carboxamides Id-IIId, IIe,f. Reactions of Ic and IIc with human serum albumin and bovine γ-globulin at pH 9.2, followed by hydrolysis of the N- or O-acetyl groups at pH 9.5, gave 50% up to 64% yields of the respective conjugates Ig, IIg and Ih, IIh.

scholarly journals Fractionation of human parotid saliva proteins.

1978 ◽  
Vol 253 (20) ◽  
pp. 7556-7565 ◽  
Author(s):  
R.I. Henkin ◽  
R.E. Lippoldt ◽  
J. Bilstad ◽  
R.O. Wolf ◽  
C.K. Lum ◽  
...  
Keyword(s):  
Parotid Saliva ◽  
Human Parotid Saliva

Substrate Specificity of Fucosyltransferase Purified from Human Parotid Saliva

1987 ◽  
Vol 66 (3) ◽  
pp. 756-760 ◽  
Author(s):  
H. Tamagawa ◽  
K. Iwakura ◽  
A. Amano ◽  
S. Shizukuishi ◽  
A. Tsunemitsu
Keyword(s):  
Substrate Specificity ◽  
Parotid Saliva ◽  
Human Parotid Saliva

Studies on histidine-rich polypeptides from human parotid saliva

1976 ◽  
Vol 177 (2) ◽  
pp. 427-436 ◽  
Author(s):  
Bruce J. Baum ◽  
Janice L. Bird ◽  
David B. Millar ◽  
Robert W. Longton
Keyword(s):  
Parotid Saliva ◽  
Human Parotid Saliva

Effect of age on immunoglobulin A subclass distribution in human parotid saliva

2003 ◽  
Vol 18 (5) ◽  
pp. 298-301 ◽  
Author(s):  
N. K. Childers ◽  
C. Greenleaf ◽  
F. Li ◽  
A. P. Dasanayake ◽  
W. D. Powell ◽  
...  
Keyword(s):  
Immunoglobulin A ◽  
Parotid Saliva ◽  
Human Parotid Saliva ◽  
Effect Of Age
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