Long-term persistence of bovine serum albumin when injected into the amphibianXenopus laevis Daudin

1968 ◽  
Vol 24 (9) ◽  
pp. 955-956 ◽  
Author(s):  
J. D. Horton
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum

Biodistribution and long-term fate of silver nanoparticles functionalized with bovine serum albumin in rats

Metallomics ◽  
2010 ◽  
Vol 2 (3) ◽  
pp. 204-210 ◽  
Author(s):  
Lourdes Garza-Ocañas ◽  
Domingo A. Ferrer ◽  
Justin Burt ◽  
Luis A. Diaz-Torres ◽  
Mónica Ramírez Cabrera ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum

Rat carcinoma cells in long-term, serum-free culture provide a continuing supply of collagenase

1985 ◽  
Vol 5 (12) ◽  
pp. 1071-1077 ◽  
Author(s):  
Geoffrey A. Stevenson ◽  
J. Guy Lyons ◽  
David A. Cameron ◽  
Robert L. O'Grady
Keyword(s):  
Epithelial Cells ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Mammary Carcinoma ◽  
Bovine Serum ◽  
Defined Medium ◽  
Carcinoma Cells ◽  
Serum Free ◽  
Vertebrate Collagenase

Neoplastic, epithelial cells derived from a spontaneously-arising rat mammary carcinoma have been cultured in a defined medium, in the absence of serum, continuously, for over 2 years. The medium is a mixture of Ham's F12 and Dulbecco's Modified Eagle's media supplemented with insulin, transferrin and bovine serum albumin. The cells have retained their potential to produce tumours and, in culture, a true vertebrate collagenase. This system provides a continuing supply of vertebrate collagenase through the application of recently developed methods.

Suppression of ovine plasma FSH by bovine follicular fluid: neutralization by plasma from ewes immunized against an inhibin-enriched preparation from bovine follicular fluid

1986 ◽  
Vol 111 (1) ◽  
pp. 1-5 ◽  
Author(s):  
S. A. R. Al-Obaidi ◽  
B. M. Bindon ◽  
M. A. Hillard ◽  
T. O'Shea ◽  
L. R. Piper
Keyword(s):  
Plasma Concentration ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Affinity Chromatography ◽  
Follicular Fluid ◽  
Bovine Serum ◽  
Luteal Phase ◽  
Plasma Concentrations

ABSTRACT Adult Merino ewes were immunized against an inhibin-enriched preparation (bFFI) obtained by affinity chromatography of bovine follicular fluid (bFF). Plasma was obtained in early luteal phase from these ewes and from control ewes immunized against bovine serum albumin. Ten months after ovariectomy the plasma concentration of FSH, but not LH, in control ewes was decreased by four s.c. injections of 8 ml bFFI (17 500 units inhibin/injection). There was no decrease in plasma concentrations of FSH or LH in immunized ewes with the same dose of bFFI. In a second study with long-term ovariectomized ewes, four injections of 20 ml plasma from the immunized ewes significantly reduced the decrease in FSH concentration caused by four injections of steroid-free bFF (2500 units inhibin/injection) in comparison with similar ewes injected with plasma from control ewes. These results show that the plasma of ewes immunized against bFFI contains substances, presumably antibodies, which neutralize the FSH-suppressive effects of bFF and bFFI in ovariectomized ewes. J. Endocr. (1986) 111, 1–5

Improvement of an artificial test substrate technique for evaluation of em immunocytochemical labeling

1987 ◽  
Vol 45 ◽  
pp. 762-763
Author(s):  
G. D. Gagne ◽  
M. F. Miller
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Artificial Substrate ◽  
Chemical Fixation ◽  
As If

We recently described an artificial substrate system which could be used to optimize labeling parameters in EM immunocytochemistry (ICC). The system utilizes blocks of glutaraldehyde polymerized bovine serum albumin (BSA) into which an antigen is incorporated by a soaking procedure. The resulting antigen impregnated blocks can then be fixed and embedded as if they are pieces of tissue and the effects of fixation, embedding and other parameters on the ability of incorporated antigen to be immunocyto-chemically labeled can then be assessed. In developing this system further, we discovered that the BSA substrate can also be dried and then sectioned for immunolabeling with or without prior chemical fixation and without exposing the antigen to embedding reagents. The effects of fixation and embedding protocols can thus be evaluated separately.

Stability of Prostacyclin in Human Plasma and Whole Blood: Studies on the Protective Effect of Albumin

1981 ◽  
Vol 46 (03) ◽  
pp. 645-647 ◽  
Author(s):  
M A Orchard ◽  
C Robinson
Keyword(s):  
Platelet Aggregation ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Protective Effect ◽  
Whole Blood ◽  
Bovine Serum ◽  
Fatty Acid Content ◽  
Krebs Solution ◽  
Antiaggregatory Activity ◽  
Free Plasma

SummaryThe biological half-life of prostacyclin in Krebs solution, human cell-free plasma or whole blood was measured by bracket assay on ADP-induced platelet aggregation. At 37°C, pH 7.4, plasma and blood reduced the rate of loss of antiaggregatory activity compared with Krebs solution. The protective effect of plasma was greater than that of whole blood. This effect could be partially mimicked by the addition of human or bovine serum albumin to the Krebs solution. The stabilisation afforded by human serum albumin was dependent on the fatty acid content of the albumin, although this was less important for bovine serum albumin.

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