Effects of estradiol and some antiestrogens (Clomiphene, tamoxifen, and hydroxytamoxifen) on luteinizing hormone secretion by rat pituitary cells in culture

1986 ◽  
Vol 237 (4) ◽  
pp. 199-211 ◽  
Author(s):  
G. Emons ◽  
O. Ortmann ◽  
Sabine Thiessen ◽  
R. Knuppen
Keyword(s):  
Luteinizing Hormone ◽  
Hormone Secretion ◽  
Pituitary Cells ◽  
Luteinizing Hormone Secretion ◽  
Cells In Culture ◽  
Rat Pituitary ◽  
Rat Pituitary Cells

Ovarian steroids modulate endothelin-induced luteinizing hormone secretion from cultured rat pituitary cells.

Endocrinology ◽  
1993 ◽  
Vol 133 (6) ◽  
pp. 2632-2638 ◽  
Author(s):  
O Ortmann ◽  
D Wassmann ◽  
S S Stojilkovic ◽  
K J Catt ◽  
K D Schulz ◽  
...  
Keyword(s):  
Luteinizing Hormone ◽  
Hormone Secretion ◽  
Pituitary Cells ◽  
Luteinizing Hormone Secretion ◽  
Ovarian Steroids ◽  
Rat Pituitary ◽  
Rat Pituitary Cells

Short-term effects of oestradiol and 4-hydroxyoestradiol on gonadotrophin-releasing hormone induced luteinizing hormone secretion by rat pituitary cells in culture

1986 ◽  
Vol 111 (3) ◽  
pp. 312-320 ◽  
Author(s):  
G. Emons ◽  
O. Ortmann ◽  
U. Fingscheidt ◽  
P. Ball ◽  
R. Knuppen
Keyword(s):  
Hormone Secretion ◽  
Female Rats ◽  
Pituitary Cells ◽  
Luteinizing Hormone Secretion ◽  
Releasing Hormone ◽  
Rat Pituitary ◽  
The Media ◽  
Gonadotrophin Releasing Hormone ◽  
Rat Pituitary Cells ◽  
Sensitizing Effect

Abstract. Dispersed pituitary cells from adult female rats were preincubated for different time periods (0– 12 h) in the absence or presence of 10−9 moestradiol (E2) or 4-hydroxyoestradiol (4-OHE2). Then the media were changed and the cells incubated for 4 h with either vehicle, or E2, or 4-OHE2 and additionally with different concentrations (10−11– 10−7 m) of gonadotrophin-releasing hormone (GnRH). Treatment of pituitary cells with E2 for 4 h (i.e. no preincubation with E2) significantly decreased the LH-response to GnRH at concentrations ≥ 10−10 m of the decapeptide. During a transition time of approximately 10 h (i.e. in cultures preincubated with E2 or vehicle for 2, 4, 6 or 8 h and then coincubated with E2 or vehicle and GnRH for 4 h) no differences between E2-and vehicle-treated cultures were observed. After 14 and 16 h of E2-treatment (i.e. 10 or 12 h preincubation and 4 h coincubation with GnRH) the LH-responses to GnRH in these cultures were significantly higher than in the respective controls. A nearly identical reaction pattern was observed when 4-OHE2 was used instead of E2. In a second series of experiments dispersed rat pituitary cells were suspended in a carrier gel and continuously perifused with medium, using small chromatography columns. When these cells were exposed for 4 min to 10−9 m GnRH at 60 or 48 min intervals, they reacted with reproducible pulsatile LH-discharges during at least 6 subsequent stimuli with the decapeptide. When E2 (10−9 m) was added to the perifusion medium, the LH-responses to GnRH were significantly reduced, starting 36 min after the onset of E2-treatment. These data indicate: 1) In the rat, the negative oestrogen effect is at least in part directly mediated at the pituitary level. 2) The sensitizing effect of oestrogens on rat gonadotrophs to GnRH is significant already after 14 to 16 h. 3) E2 and the catecholoestrogen 4-OHE2 have the same effects in this system. 4) The negative E2-effect on GnRH-induced LH-release is significant after only 36 min, a finding bringing up the question of a non-genomic mechanism.

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