4. Influence of sterilization and temperature changes on the in vitro characteristics of the pH electrode

1978 ◽  
Vol 226 (1-2) ◽  
pp. 25-30
Author(s):  
H. Rüttgers ◽  
D. Muliawan ◽  
U. Boos ◽  
F. Kubli
10.2341/05-26 ◽  
2006 ◽  
Vol 31 (2) ◽  
pp. 261-265 ◽  
Author(s):  
A. R. Yazici ◽  
A. Müftü ◽  
G. Kugel ◽  
R. D. Perry

Clinical Relevance The thickness of the residual dentin is a critical factor in the reducing thermal transfer to pulp, and this transfer varies with the curing unit used.


1983 ◽  
Vol 245 (1) ◽  
pp. R32-R37
Author(s):  
P. J. Walsh ◽  
T. W. Moon

The effects of acclimation temperature and acute temperature changes on the intracellular pH (pHi) of hepatocytes isolated from the American eel, Anguilla rostrata, were studied by the measurement of the distribution ratio of dimethyloxizolidinedione (DMO). Varying the concentration of DMO (10(-7) to 10(-4) M) did not affect estimates of pHi, indicating that DMO acts as an ideal pHi probe in eel hepatocytes. In vitro studies yielded values of liver cell pHi identical to those previously measured in vivo (in vitro pHi = 7.556 +/- 0.010; in vivo pHi = 7.570 +/- 0.049 at 20 degrees C); hepatocyte pHi varied inversely with acclimation temperature (5-20 degrees C) in a manner consistent with alphastat regulation (delta pH/delta T = -0.0182 +/- 0.021). During acute temperature increases (5-20 degrees C) and decreases (20-5 degrees C) hepatocytes regulated pHi to the appropriate (acclimated) value within 30-45 min posttransfer under conditions of constant medium pH (pHe). The effects of medium pH were also studied, and although patterns of pHi regulation differed between 5 and 20 degrees C cells, a pHi difference consistent with alphastat regulation was maintained between 5 and 20 degrees C cells over the pHe range 7.8-8.3.


The Analyst ◽  
2018 ◽  
Vol 143 (13) ◽  
pp. 3124-3133 ◽  
Author(s):  
Niall J. Finnerty ◽  
Fiachra B. Bolger
Keyword(s):  

Herein, we report the in vitro investigation of a physiologically relevant Pt oxide-based microvoltammetric pH electrode.


1997 ◽  
Vol 21 (5) ◽  
pp. 464-473 ◽  
Author(s):  
Paul Steiner ◽  
Andreas W. Schoenenberger ◽  
Peter Erhart ◽  
Erik Penner ◽  
Gustav K. von Schulthess ◽  
...  
Keyword(s):  

1947 ◽  
Vol 85 (6) ◽  
pp. 741-757 ◽  
Author(s):  
Theodore T. Puck

The effectiveness of any compound as an aerial germicide depends upon the extent of condensation of its vapor on air-suspended bacteria, and on the rate at which the resulting concentration of germicide can produce death of the microorganisms. The properties of any compound conducive to production of the highest rate of kill of air-borne microorganisms by means of the smallest possible concentration of germicide vapor, are as folows: (a) a low vapor pressure, but not lower than 0.001 mm. Hg at 25°C.; (b) high hygroscopicity; (c) toxicity for bacterial metabolism—a high degree of potency is not necessary although the killing action will be more efficient the higher the antibacterial activity of the compound employed. For any compound the killing action is always a direct function of the concentration of its vapor in the air. The maximum amount of a hygroscopic substance which can exist in the vapor state decreases as the relative humidity increases. Hence, at high relative humidities the bactericidal efficiency is lowest. At lower relative humidities the air can contain more vapor, and hence a greater effect is possible. At any relative humidity, the killing action is greater, the more closely the germicide vapor concentration approaches the saturation point. The presence of soluble compounds in droplets containing bacteria promotes more extensive condensation of the germicide than would otherwise occur, and so enhances its effectiveness. In the absence of such soluble substances, low atmospheric humidities may cause complete desiccation of a bacterial particle. Under these conditions its surface may become resistant to the condensation of the vapor, and thus prevent effective germicidal action. The influence of temperature changes on the killing efficiency maybe correctly deduced from a consideration of the effect of a rise in temperature on the vapor pressure of the germicide and on the rate of its bactericidal action in vitro. Equations are presented for estimating quantitatively the magnitude of some of the effects discussed.


2003 ◽  
Author(s):  
Jesus Djalma Pecora ◽  
Aldo Brugnera, Jr. ◽  
Melissa A. Marchesan ◽  
Danilo M. Zanello Guerisoli ◽  
Eduardo L. Barbin ◽  
...  

2012 ◽  
Vol 41 (11) ◽  
pp. 1338-1343 ◽  
Author(s):  
S. Schütz ◽  
J. Egger ◽  
S. Kühl ◽  
A. Filippi ◽  
J.Th. Lambrecht
Keyword(s):  

Author(s):  
Enrico Di Stasio ◽  
Patrizia Bizzarri ◽  
Milvia Casato ◽  
Antonio Galtieri ◽  
Massimo Fiorilli ◽  
...  

AbstractCryoglobulins are pathological cold-precipitable immunoglobulins associated with a number of infectious, autoimmune and neoplastic disorders. Patients, when exposed to low temperatures, show symptoms related to intravascular precipitation of such immunoglobulins. The formation of cryoaggregates induced by exposure to cold temperature is the key pathogenetic mechanism. The subsequent intravascular precipitation can account for some clinical signs of peripheral vasculitis, but fails to explain the precipitation of cryoglobulins in regions where no significant temperature changes take place. We studied, in vitro, the activity of different ions on temperature-dependent aggregation of cryoglobulins and found that the concentration of Cl


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