Inhibition of red light-induced seed germination by indole-3-acetic acid inHygrophila auriculata

1992 ◽  
Vol 11 (4) ◽  
pp. 203-208
Author(s):  
Dilip Amritphale ◽  
Bharat Singh ◽  
Aarti Gutch
Keyword(s):  
Acetic Acid ◽  
Seed Germination ◽  
Red Light ◽  
Indole 3 Acetic Acid

scholarly journals Non-symbiotic Seed Germination and In vitro Plant Development of Pholidota articulata

2021 ◽  
Vol 15 ◽  
pp. 44-51
Author(s):  
R. Devendra Prasad ◽  
Shreeti Pradan ◽  
Mukti Ram Poudel ◽  
Bijaya Pant
Keyword(s):  
Acetic Acid ◽  
Seed Germination ◽  
Plant Production ◽  
Sustainable Utilization ◽  
Ms Medium ◽  
Natural Habitats ◽  
Half Strength ◽  
Indole 3 Acetic Acid ◽  
Symbiotic Seed Germination

Pholidota articulata is an epiphytic orchid mostly used in ornamental cut/pot flower and in traditional medicine. As it has high ornamental and medicinal values, its population from natural habitats is decreasing, therefore, it is listed in the Appendix-II of Convention on International Trade in Endangered Species (CITES). The objective of the present study is to obtain the in vitro plants of P. articulata from seed culture to conserve its germplasm. The in vitro seed germination was carried out in different strengths of Murashige and Skoog (MS) and Knudson C (KnC) medium supplemented with various plant hormones. On the half-strength of MS medium, seeds were started to germinate after 4 weeks of primary culture and they were developed into protocorms with first leaf primordium earlier than on the other medium. Therefore, in vitro developed protocorms were sub-cultured on the half-strength of MS medium supplemented with different concentrations of 6-benzylaminopurine (BAP), gibberellic acid (GA3) and α-naphtalene acetic acid (NAA). They were successfully developed into shoots on the 1.5 mg/l BAP supplemented half-strength of MS medium. Later, they were inoculated on the half-strength of MS medium supplemented with different concentration of α-napthalene acetic acid (NAA), indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) for the root formation, where IBA supplemented medium was found effective for the development of roots. Thus, this study provides a reliable protocol for non-symbiotic seed germination and plant production, and reveals that seed-derived protocorms are good explants for the in vitro mass propagation for conservation and sustainable utilization in horticulture.

scholarly journals Effect of Gibberellic Acid and Indole 3-Acetic Acid on Seed Germination Performance of Horse gram (Macrotyloma uniflorum) Lam (Verdc)

2016 ◽  
Vol 1 (2) ◽  
pp. 36 ◽  
Author(s):  
J. Lalitha ◽  
Haseena Rafath ◽  
M. Subash
Keyword(s):  
Acetic Acid ◽  
Seed Germination ◽  
Plant Growth ◽  
Gibberellic Acid ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Germination Percentage ◽  
Potential Growth ◽  
Macrotyloma Uniflorum ◽  
Indole 3 Acetic Acid

The experiment was undertaken with an objective to investigate the effect of various concentrations of plant growth regulators, i.e., Gibberellic acid (GA3) and Indole 3-acetic acid (IAA) on seed germination of Macrotyloma uniflorum. Seeds were soaked for 12 hours in different concentrations substances (1.0, 2.0, 3.0 and 4.0 mg/L) of IAA, GA3 and control set was soaked only in distilled water.  Three replicates of each treatment with fifty seeds per replicate were arranged for precise physiological analysis. Significant variation was found in all aspects after analysis of variance (ANOVA) of each mean value. After two weeks of seed soaking, it was noted that germination percentages were significantly accelerated by lower concentrations (1 and 2 mg) of used hormones. Amongst the two potential growth regulators, 2 mg/L was found most effective because it showed highest germination percentage for IAA (93%) and GA3 (88%). A great deal of information relating to seed germination practices shows that these plant growth regulators were efficient in overcoming dormancy leading to rapid seed germination. IAA was selected as best hormone in this study, which showed highest seed germination (93%). 

scholarly journals Gliding Arc Plasma Treatment of Maize (Zea mays L.) Grains Promotes Seed Germination and Early Growth, Affecting Hormone Pools, but Not Significantly Photosynthetic Parameters

Agronomy ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2066
Author(s):  
Božena Šerá ◽  
Radomíra Vanková ◽  
Karel Roháček ◽  
Michal Šerý
Keyword(s):  
Acetic Acid ◽  
Abscisic Acid ◽  
Seed Germination ◽  
Jasmonic Acid ◽  
Plasma Treatment ◽  
Photosynthetic Parameters ◽  
Arc Plasma ◽  
Gliding Arc ◽  
Gliding Arc Plasma ◽  
Indole 3 Acetic Acid

Maize grains (Zea mays convar. Indentata Sturt.) were treated with non-thermal plasma, where Gliding Arc plasma discharge at an atmospheric pressure was used (working gas: Air; time duration: 0 s, 180 s, 300 s, 600 s). The experiment was conducted at a temperature of 18 °C, light/dark 12/12 h, and a light intensity of 100 µmol/m2s. Seed germination, seedling growth, photosynthetic parameters, and hormone (abscisic acid, jasmonic acid, salicylic acid, indole-3-acetic acid, and cytokinin) contents were measured. The highest stimulation of seed germination (to 141%), root length (to 221%), shoot length (to 298%), and root weight (to 122%) in comparison with the control was recorded after Gliding Arc plasma treatment for 600 s. The photochemical and non-photochemical Chl fluorescence parameters were not significantly affected by Gliding Arc plasma treatment. In contrast, hormonal pools in maize were significantly affected. The short-term plasma treatment (180 s) was associated with a decrease in the stress hormones abscisic acid, salicylic acid, jasmonic acid, and jasmonate isoleucine, while indole-3-acetic acid and cytokinin precursors were elevated. Longer-term treatment (300 s, 600 s) had an opposite effect—an elevation of abscisic acid, jasmonic acid, and jasmonate isoleucine as well as active cytokinins. The content of auxin decreased. Gliding plasma treatment may significantly affect maize physiology, dependent on the treatment duration.

Orchid-associated bacteria produce indole-3-acetic acid, promote seed germination, and increase their microbial yield in response to exogenous auxin

2007 ◽  
Vol 188 (6) ◽  
pp. 655-664 ◽  
Author(s):  
Elena A. Tsavkelova ◽  
Tatiana A. Cherdyntseva ◽  
Svetlana Yu. Klimova ◽  
Andrey I. Shestakov ◽  
Svetlana G. Botina ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Seed Germination ◽  
Associated Bacteria ◽  
Exogenous Auxin ◽  
Indole 3 Acetic Acid

scholarly journals Protocorm-like Bodies Initiation from Root Tips of Cyrtopodium paranaense (Orchidaceae)

HortScience ◽  
2010 ◽  
Vol 45 (9) ◽  
pp. 1365-1368 ◽  
Author(s):  
Wei-Ling Guo ◽  
Yao-Chien Alex Chang ◽  
Chien-Yuan Kao
Keyword(s):  
Acetic Acid ◽  
Seed Germination ◽  
Synergistic Effect ◽  
Early Stage ◽  
Root Apex ◽  
Root Tip ◽  
Ms Medium ◽  
Root Tips ◽  
Terrestrial Orchid ◽  
Indole 3 Acetic Acid

Cyrtopodium paranaense is a tropical terrestrial orchid, which propagates mainly through sexual seed germination. In this study, we document the asexual morphogenesis of the root tip to protocorm-like body (PLB) conversion in Cyrtopodium paranaense. Protocorm-like bodies sporadically developed from root tips of flask-grown seedlings in the absence of any exogenous plant growth regulators (PGRs). The compact PLBs ultimately gave rise to normal plantlets. Histological observations revealed that the root cap became dissociated from the root apex at an early stage followed by dispersed extension of root vascular strands into nascent PLBs. Protocorm-like bodies also developed from the root central stele tissue. In root tip segment cultures, PLBs were not formed without providing PGRs but were efficiently formed from root tips in Murashige and Skoog (MS) medium supplemented with 10.2 μM indole-3-acetic acid (IAA) and 9.0 μM thidiazuron (TDZ). Both IAA and TDZ promoted the formation of PLBs; however, TDZ did not induce PLB formation in the absence of IAA, indicating a synergistic effect of the two PGRs. Protocorm-like bodies were proliferated and subsequently plants regenerated in PGR-free MS medium. Root tip culture may be used as an alternative method for the propagation of Cyrtopodium paranaense.

scholarly journals Indole-3-acetic acid (IAA) producing Pseudomonas isolates inhibit seed germination and α-amylase activity in durum wheat (Triticum turgidum L.)

2016 ◽  
Vol 14 (1) ◽  
pp. e0802 ◽  
Author(s):  
Samira Tabatabaei ◽  
Parviz Ehsanzadeh ◽  
Hassan Etesami ◽  
Hossein A. Alikhani ◽  
Bernard R. Glick
Keyword(s):  
Acetic Acid ◽  
Seed Germination ◽  
Durum Wheat ◽  
Triticum Turgidum ◽  
Amylase Activity ◽  
Inhibitory Effect ◽  
Bacterial Isolates ◽  
Indole 3 Acetic Acid

<p>The role of plant-associated bacteria in plant physiology and metabolism is well documented, but little has been known about the roles played by <em>Pseudomonas</em> in durum wheat (<em>Triticum turgidum</em> L. var <em>durum</em>) growth and development. An<em> in vitro</em>experiment was conducted to observe the effect of the inoculation of four indole-3-acetic acid (IAA)-producing <em>Pseudomonas </em>isolates<em> </em>and exogenous IAA on seed germination traits and α-amylase activity of durum wheat. The results showed inoculation with all bacterial isolates led to a decrease in the germination percent, although the extent of the depression varied with the isolate. A significant relationship between concentrations of bacterial IAA and the germination inhibition percent in durum wheat seeds by different bacteria strains was observed. The results of this assay showed the effect of bacterial isolates on α-amylase activity after six and 8 days of inoculation was significant, while effect of these isolates on α-amylase activity after two and 4 days of inoculation was not meaningful. In addition, the exogenously applied IAA displayed a concentration-dependent effect on seed germination attributes and α-amylase activity, consistent with the possibility that the inhibitory effect of bacterial inoculation on seed germination was in consequence of bacteria-produced IAA. Therefore, it may suggested that the inhibitory role of IAA in seed germination and α-amylase activity should be taken into account during the screening of IAA-producing <em>Pseudomonas</em> isolates for durum wheat growth promoting agents.</p>

Sign in / Sign up

Export Citation Format

Share Document