Nucleotide sequence of a DNA region comprising the gene for elongation factor 1α (EF-1α) from the ultrathermophilic archaeotePyrococcus woesei: Phylogenetic implications

1991 ◽  
Vol 33 (4) ◽  
pp. 332-342 ◽  
Author(s):  
Roberta Creti ◽  
Franca Citarella ◽  
Orsola Tiboni ◽  
Annamaria Sanangelantoni ◽  
Peter Palm ◽  
...  
Genome ◽  
1997 ◽  
Vol 40 (4) ◽  
pp. 559-565 ◽  
Author(s):  
Peter S. Nielsen ◽  
Andris Kleinhofs ◽  
Odd-Arne Olsen

A full length cDNA clone encoding the 447 amino acid long barley (Hordeum vulgare cv. Bomi) endosperm elongation factor 1α (eF-1α) was isolated by a differential screening procedure. RFLP mapping of eF-1α showed that the barley genome contains a small eF-1α gene family of 4 copies, with 1 copy of the gene being located on each of chromosomes 2, 4, 6, and 7. Analysis of barley endosperm total proteins by Western blot with antibodies directed towards wheat eF-1α and the sea urchin 51 kDa proteins gave a single band of the expected molecular weight. Amino acid sequence comparison with other plant eF-1α sequences showed that the isolated barley endosperm eF-1α is more similar to the published wheat eF-1α sequence than to eF-1α sequences previously published for the barley cultivars Igri and Dicktoo. The phylogenetic analysis suggests that the barley eF-1α gene family can be divided into two subfamilies and that two ancestral genes existed before the divergence of monocotyledonous and dicotyledonous plants.Key words: endosperm, gene family, RFLP.


1992 ◽  
Vol 20 (6) ◽  
pp. 1422-1422 ◽  
Author(s):  
Jens Cavallius ◽  
William C. Merrick

1992 ◽  
Vol 20 (4) ◽  
pp. 909-909 ◽  
Author(s):  
Takuji Shirasawa ◽  
Kyoichi Sakamoto ◽  
Takumi Akashi ◽  
Hiroshi Takahashi ◽  
Akira Kawashima

1993 ◽  
Vol 21 (3) ◽  
pp. 742-742 ◽  
Author(s):  
Katsuyoshi Kamiie ◽  
Hideharu Taira ◽  
Hirokatsu Ooura ◽  
Asako Kakuta ◽  
Shogo Matsumoto ◽  
...  

1985 ◽  
Vol 260 (5) ◽  
pp. 3090-3096
Author(s):  
P Cottrelle ◽  
D Thiele ◽  
V L Price ◽  
S Memet ◽  
J Y Micouin ◽  
...  

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