Detection of JC virus by polymerase chain reaction in cerebrospinal fluid from two patients with progressive multifocal leukoencephalopathy

1992 ◽  
Vol 11 (3) ◽  
pp. 253-254 ◽  
Author(s):  
A. Telenti ◽  
W. F. Marshall ◽  
A. J. Aksamit ◽  
J. D. Smilack ◽  
T. F. Smith
Keyword(s):  
Polymerase Chain Reaction ◽  
Cerebrospinal Fluid ◽  
Progressive Multifocal Leukoencephalopathy ◽  
Jc Virus ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Improving detection of JC virus by ultrafiltration of cerebrospinal fluid before polymerase chain reaction for the diagnosis of progressive multifocal leukoencephalopathy

BMC Neurology ◽  
2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Kazuo Nakamichi ◽  
Michi Kawamoto ◽  
Junko Ishii ◽  
Masayuki Saijo
Keyword(s):  
Polymerase Chain Reaction ◽  
Cerebrospinal Fluid ◽  
Detection Limit ◽  
Progressive Multifocal Leukoencephalopathy ◽  
Real Time ◽  
Real Time Pcr ◽  
Jc Virus ◽  
Standard Procedure ◽  
Chain Reaction ◽  
Polymerase Chain

Abstract Background Progressive multifocal leukoencephalopathy (PML) is a demyelinating disorder caused by JC virus (JCV). Although detecting JCV DNA in the cerebrospinal fluid (CSF) by real-time polymerase chain reaction (PCR) is useful, diagnosis is difficult when JCV concentrations are low. We therefore aimed to lower the detection limit of real-time PCR testing by enriching JCV in the CSF via ultrafiltration. Methods Virus suspensions and CSF specimens from 20 untreated patients with suspected PML were collected and total DNAs were extracted. The JCV large T gene was detected by quantitative real-time PCR under condition with and without prior centrifugal ultrafiltration. Results The JCV DNA was reliably detected to a lower limit of 10 copies/mL of virus suspension by real-time PCR with ultrafiltration. When using this method, the quantity of JCV DNA per PCR reaction increased 3.2- to 8.7-fold compared with the standard procedure. Seven patients were positive for JCV when using the standard procedure, and an additional patient was positive when using ultrafiltration. All JCV-positive patients had neurological features and magnetic resonance imaging findings compatible with PML. Conclusions The detection limit of JCV DNA by real-time PCR can be lowered by viral enrichment using ultrafiltration. Our simple protocol offers a valuable tool for PML diagnosis when extremely low copy numbers of JCV are released into the CSF or when brain biopsy is not feasible.

Polymerase chain reaction for detection of JC virus DNA in cerebrospinal fluid: a quality control study

1997 ◽  
Vol 69 (1-2) ◽  
pp. 231-237 ◽  
Author(s):  
T. Weber ◽  
P.E. Klapper ◽  
G.M. Cleator ◽  
M. Bodemer ◽  
W. Lüke ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Quality Control ◽  
Cerebrospinal Fluid ◽  
Jc Virus ◽  
Chain Reaction ◽  
Quality Control Study ◽  
Polymerase Chain ◽  
Control Study

scholarly journals JC Virus Granule Cell Neuronopathy and Lymphoma

2018 ◽  
Vol 5 (6) ◽  
Author(s):  
Alexis Demas ◽  
Omar Bennani ◽  
Anne Vandendriessche ◽  
Laurence Hellouin de Menibus ◽  
Vincent Langlois ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cerebrospinal Fluid ◽  
Granule Cell ◽  
Opportunistic Infections ◽  
Jc Virus ◽  
Immunocompromised Patient ◽  
Chain Reaction ◽  
Progressive Cerebellar Ataxia ◽  
Polymerase Chain ◽  
Granule Cell Neuronopathy

Abstract Neurological opportunistic infections are going to increase. Clinicians should be aware of the neurological spectrum of JC virus manifestations, including granule cell neuronopathy. Detection of JC virus DNA by polymerase chain reaction in cerebrospinal fluid should be realized in the assessment of a progressive cerebellar ataxia in an immunocompromised patient.

scholarly journals Nearly one-half of Brazilian patients with multiple sclerosis using natalizumab are DNA-JC virus positive

2013 ◽  
Vol 71 (10) ◽  
pp. 780-782 ◽  
Author(s):  
Yara Dadalti Fragoso ◽  
Maria Fernanda Mendes ◽  
Walter Oleschko Arruda ◽  
Jefferson Becker ◽  
Joseph Bruno Bidin Brooks ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Polymerase Chain Reaction ◽  
Progressive Multifocal Leukoencephalopathy ◽  
Real Time ◽  
Jc Virus ◽  
Chain Reaction ◽  
Efficient Treatment ◽  
Qualitative Detection ◽  
Polymerase Chain

Objective Natalizumab is a new and efficient treatment for multiple sclerosis (MS). The risk of developing progressive multifocal leukoencephalopathy (PML) during the use of this drug has created the need for better comprehension of JC virus (JCV) infection. The objective of the present study was to assess the prevalence of JCV-DNA in Brazilian patients using natalizumab. Method Qualitative detection of the JCV in the serum was performed with real-time polymerase chain reaction (PCR). Results In a group of 168 patients with MS who were undergoing treatment with natalizumab, JCV-DNA was detectable in 86 (51.2%) patients. Discussion Data on JCV-DNA in Brazil add to the worldwide assessment of the prevalence of the JCV in MS patients requiring treatment with natalizumab.

Sign in / Sign up

Export Citation Format

Share Document