Evidences against a role for adenine methylation in the tryptophan biosynthetic pathway inEscherichia coli and for a growth phase-dependent induction of thetrp promoter

1991 ◽  
Vol 23 (1) ◽  
pp. 21-25
Author(s):  
Frédéric Barras ◽  
Mireille Magnan ◽  
Martin G. Marinus
Keyword(s):  
Growth Phase ◽  
Biosynthetic Pathway ◽  
Inescherichia Coli ◽  
Adenine Methylation

scholarly journals Growth phase dependent stop codon readthrough and shift of translation reading frame inEscherichia coli

FEBS Letters ◽  
1998 ◽  
Vol 421 (3) ◽  
pp. 237-242 ◽  
Author(s):  
Anne-Marie K Wenthzel ◽  
Martin Stancek ◽  
Leif A Isaksson
Keyword(s):  
Growth Phase ◽  
Stop Codon ◽  
Inescherichia Coli ◽  
Reading Frame ◽  
Stop Codon Readthrough

scholarly journals Growth Phase-Dependent Regulation of Vsr Endonuclease May Contribute to 5-Methylcytosine Mutational Hot Spots inEscherichia coli

1999 ◽  
Vol 181 (14) ◽  
pp. 4435-4436 ◽  
Author(s):  
Georgina Macintyre ◽  
Photini Pitsikas ◽  
Claire G. Cupples
Keyword(s):  
Escherichia Coli ◽  
Cell Growth ◽  
Growth Phase ◽  
Hot Spots ◽  
Polyclonal Antibodies ◽  
Constant Level ◽  
Inescherichia Coli

ABSTRACT Using rabbit polyclonal antibodies, we have shown that the Dcm cytosine methylase of Escherichia coli is maintained at a constant level during cell growth, while Vsr endonuclease levels are growth phase dependent. Decreased production of Vsr relative to Dcm during the log phase may contribute substantially to the mutability of 5-methylcytosine.

scholarly journals Archaeal Phospholipid Biosynthetic Pathway Reconstructed inEscherichia coli

Archaea ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Takeru Yokoi ◽  
Keisuke Isobe ◽  
Tohru Yoshimura ◽  
Hisashi Hemmi
Keyword(s):  
Recombinant Proteins ◽  
Biosynthetic Pathway ◽  
Membrane Lipids ◽  
Membrane Lipid ◽  
Ionization Mass ◽  
Inescherichia Coli ◽  
Methanosarcina Acetivorans ◽  
E Coli ◽  
Endogenous Enzymes

A part of the biosynthetic pathway of archaeal membrane lipids, comprised of 4 archaeal enzymes, was reconstructed in the cells ofEscherichia coli. The genes of the enzymes were cloned from a mesophilic methanogen,Methanosarcina acetivorans, and the activity of each enzyme was confirmed using recombinant proteins.In vitroradioassay showed that the 4 enzymes are sufficient to synthesize an intermediate of archaeal membrane lipid biosynthesis, that is, 2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate, from precursors that can be produced endogenously inE. coli. Introduction of the 4 genes intoE. coliresulted in the production of archaeal-type lipids. Detailed liquid chromatography/electron spray ionization-mass spectrometry analyses showed that they are metabolites from the expected intermediate, that is, 2,3-di-O-geranylgeranyl-sn-glycerol and 2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphoglycerol. The metabolic processes, that is, dephosphorylation and glycerol modification, are likely catalyzed by endogenous enzymes ofE. coli.

Overproduction, inEscherichia coli,of Soluble Taxadiene Synthase, a Key Enzyme in the Taxol Biosynthetic Pathway

1998 ◽  
Vol 13 (1) ◽  
pp. 90-96 ◽  
Author(s):  
Ke-xue Huang ◽  
Qiu-long Huang ◽  
Mark R. Wildung ◽  
Rodney Croteau ◽  
A.I. Scott
Keyword(s):  
Biosynthetic Pathway ◽  
Inescherichia Coli ◽  
Taxadiene Synthase ◽  
Key Enzyme

Growth-phase associated changes in the reproductive capacity, intracellular polyamines, and antilysozyme activity inEscherichia coli batch culture

Microbiology ◽  
2000 ◽  
Vol 69 (1) ◽  
pp. 113-115 ◽  
Author(s):  
O. V. Bukharin ◽  
V. A. Gritsenko ◽  
A. G. Tkachenko ◽  
O. Ya. Salakhetdinova
Keyword(s):  
Batch Culture ◽  
Growth Phase ◽  
Reproductive Capacity ◽  
Inescherichia Coli ◽  
Antilysozyme Activity
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