Location of anaerobic respiratory enzyme trimethylamine N-oxide reductase in the cytoplasmic membrane ofEscherichia coli strain K10

1991 ◽  
Vol 22 (6) ◽  
pp. 349-353
Author(s):  
Isamu Yamamoto ◽  
Masato Hinakura ◽  
Hiroyuki Kondo
1972 ◽  
Vol 18 (6) ◽  
pp. 909-915 ◽  
Author(s):  
A. P. Singh ◽  
K.-J. Cheng ◽  
J. W. Costerton ◽  
E. S. Idziak ◽  
J. M. Ingram

The site of the cell barrier to actinomycin-D uptake was studied using a wild-type Escherichia coli strain P and its cell envelope-defective filamentous mutants, strains 6γ and 12γ, both of which 'leak' β-galactosidase and alkaline phosphatase into the medium during growth indicating both membrane and cell-wall defects. Actinomycin-D entered the cells of these two mutant strains as evidenced by the inhibition of both 14C-uracil incorporation and synthesis of the induced β-galactosidase system. Under similar conditions, no inhibition occurred in the wild-type strain and its sucrose-lysozyme prepared spheroplasts. Actinomycin-D did, however, inhibit the above-mentioned systems in the wild-type sucrose-lysozyme spheroplasts prepared in the presence of 2 mM EDTA. The experimental data indicate that although the cell wall may act as a primary barrier or sieve to actinomycin-D, the cytoplasmic membrane should be considered the final and determinative barrier to this antibiotic.


Biochemistry ◽  
2005 ◽  
Vol 44 (20) ◽  
pp. 7570-7576 ◽  
Author(s):  
Jaroslaw Króliczewski ◽  
Katarzyna Hombek-Urban ◽  
Andrzej Szczepaniak

1998 ◽  
Vol 273 (27) ◽  
pp. 16897-16904 ◽  
Author(s):  
Teruo Kuroda ◽  
Naoyuki Okuda ◽  
Naoto Saitoh ◽  
Tetsuo Hiyama ◽  
Yoko Terasaki ◽  
...  

Biochemistry ◽  
2002 ◽  
Vol 41 (17) ◽  
pp. 5605-5612 ◽  
Author(s):  
Janek Kubelt ◽  
Anant K. Menon ◽  
Peter Müller ◽  
Andreas Herrmann

1993 ◽  
Vol 107 (2-3) ◽  
pp. 175-178 ◽  
Author(s):  
Dorothea Seiffer ◽  
Jürgen Robert Klein ◽  
Roland Plapp

1995 ◽  
Vol 270 (40) ◽  
pp. 23485-23490 ◽  
Author(s):  
Yoshinori Akiyama ◽  
Tohru Yoshihisa ◽  
Koreaki Ito

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