Depletion of catalytic and regulatory subunits of protein kinase CK2 by antisense oligonucleotide treatment of neuroblastoma cells

1994 ◽  
Vol 14 (5) ◽  
pp. 407-414 ◽  
Author(s):  
Luis Ulloa ◽  
Javier Díaz-Nido ◽  
Jesus Avila
Keyword(s):  
Protein Kinase ◽  
Antisense Oligonucleotide ◽  
Protein Kinase Ck2 ◽  
Neuroblastoma Cells ◽  
Regulatory Subunits ◽  
Kinase Ck2

Crystal structures of catalytic and regulatory subunits of rat protein kinase CK2

2009 ◽  
Vol 54 (2) ◽  
pp. 220-226 ◽  
Author(s):  
WeiHong Zhou ◽  
XiaoHong Qin ◽  
XiaoJie Yan ◽  
XingQiao Xie ◽  
Liang Li ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Crystal Structures ◽  
Protein Kinase Ck2 ◽  
Regulatory Subunits ◽  
Kinase Ck2

Yeast holoenzyme of protein kinase CK2 requires both β and β′ regulatory subunits for its activity

2006 ◽  
Vol 295 (1-2) ◽  
pp. 229-236 ◽  
Author(s):  
Konrad Kubiński ◽  
Katarzyna Domańska ◽  
Ewa Sajnaga ◽  
Elżbieta Mazur ◽  
Rafał Zieliński ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Protein Kinase Ck2 ◽  
Regulatory Subunits ◽  
Kinase Ck2

Assembly of protein kinase CK2: investigation of complex formation between catalytic and regulatory subunits using a zinc-finger-deficient mutant of CK2β

2001 ◽  
Vol 358 (1) ◽  
pp. 87-94 ◽  
Author(s):  
David A. CANTON ◽  
Cunjie ZHANG ◽  
David W. LITCHFIELD
Keyword(s):  
Protein Kinase ◽  
Zinc Finger ◽  
Protein Kinase Ck2 ◽  
Deficient Mutant ◽  
Binding Assays ◽  
Catalytic Subunits ◽  
Regulatory Subunits ◽  
Regulatory Ck2β Subunit ◽  
Kinase Ck2

Protein kinase CK2 is a tetrameric enzyme comprised of two regulatory subunits (CK2β) and two catalytic subunits (CK2α and/or CK2α′). The crystal structure of dimeric CK2β demonstrated that a zinc finger mediates CK2β dimerization, therefore we constructed a mutant in which cysteine residues 109 and 114 were mutated to serine. Our objectives were to examine the effects of disrupting the zinc finger of the regulatory CK2β subunit on CK2 tetramer assembly. Examination of this zinc-finger-deficient mutant of CK2β using a yeast two-hybrid assay demonstrates that the mutant fails to form CK2β homodimers. In order to extend these studies, we co-transfected COS-7 cells with epitope-tagged constructs and performed co-immunoprecipitation assays. The results from these studies demonstrate that the mutant fails to form CK2β homodimers and fails to interact with catalytic CK2 subunits. Furthermore, we demonstrate that the mutant CK2β is not appreciably phosphorylated in cells. Using in vitro binding assays, we demonstrated that the mutant CK2β protein fails to interact with glutathione S-transferase–CK2α′. Finally, we demonstrate that the mutant is translated at an equivalent rate to wild-type CK2β, but is degraded much more rapidly. Overall, our results are consistent with the model that β–β dimerization precedes incorporation of catalytic subunits into tetrameric CK2 complexes, and that β–β dimerization is a prerequisite for the stable incorporation of catalytic subunits into CK2 complexes.

scholarly journals The Alpha Catalytic Subunit of Protein Kinase CK2 Is Required for Mouse Embryonic Development

2007 ◽  
Vol 28 (1) ◽  
pp. 131-139 ◽  
Author(s):  
David Y. Lou ◽  
Isabel Dominguez ◽  
Paul Toselli ◽  
Esther Landesman-Bollag ◽  
Conor O'Brien ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Embryonic Development ◽  
Protein Kinase Ck2 ◽  
Germ Line ◽  
Cell Physiology ◽  
Mammalian Development ◽  
Serine Threonine Kinase ◽  
Regulatory Subunits ◽  
Neural Tubes ◽  
Kinase Ck2

ABSTRACT Protein kinase CK2 (formerly casein kinase II) is a highly conserved and ubiquitous serine/threonine kinase that is composed of two catalytic subunits (CK2α and/or CK2α′) and two CK2β regulatory subunits. CK2 has many substrates in cells, and key roles in yeast cell physiology have been uncovered by introducing subunit mutations. Gene-targeting experiments have demonstrated that in mice, the CK2β gene is required for early embryonic development, while the CK2α′ subunit appears to be essential only for normal spermatogenesis. We have used homologous recombination to disrupt the CK2α gene in the mouse germ line. Embryos lacking CK2α have a marked reduction in CK2 activity in spite of the presence of the CK2α′ subunit. CK2α−/− embryos die in mid-gestation, with abnormalities including open neural tubes and reductions in the branchial arches. Defects in the formation of the heart lead to hydrops fetalis and are likely the cause of embryonic lethality. Thus, CK2α appears to play an essential and uncompensated role in mammalian development.

Maize protein kinase CK2: regulation and functionality of three β regulatory subunits

2001 ◽  
Vol 25 (4) ◽  
pp. 365-374 ◽  
Author(s):  
Marta Riera ◽  
Giovanna Peracchia ◽  
Eulàlia De Nadal ◽  
Joaquín Ariño ◽  
Montserrat Pagès
Keyword(s):  
Protein Kinase ◽  
Protein Kinase Ck2 ◽  
Regulatory Subunits ◽  
Maize Protein ◽  
Kinase Ck2
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