A simple staining method for the morphologic classification of spermatozoa

1982 ◽  
Vol 14 (2) ◽  
pp. 185-188 ◽  
Author(s):  
M. Berényi ◽  
Gy. Corradi
Keyword(s):  
Staining Method ◽  
Morphologic Classification

scholarly journals The Banff Working Group Classification of Definitive Polyomavirus Nephropathy: Morphologic Definitions and Clinical Correlations

2017 ◽  
Vol 29 (2) ◽  
pp. 680-693 ◽  
Author(s):  
Volker Nickeleit ◽  
Harsharan K. Singh ◽  
Parmjeet Randhawa ◽  
Cinthia B. Drachenberg ◽  
Ramneesh Bhatnagar ◽  
...  
Keyword(s):  
Repeated Measures ◽  
Working Group ◽  
Classification Scheme ◽  
Clinical Presentation ◽  
Interstitial Fibrosis ◽  
The United States ◽  
Systematic Analysis ◽  
Definition Of ◽  
Morphologic Classification

Polyomavirus nephropathy (PVN) is a common viral infection of renal allografts, with biopsy-proven incidence of approximately 5%. A generally accepted morphologic classification of definitive PVN that groups histologic changes, reflects clinical presentation, and facilitates comparative outcome analyses is lacking. Here, we report a morphologic classification scheme for definitive PVN from the Banff Working Group on Polyomavirus Nephropathy, comprising nine transplant centers in the United States and Europe. This study represents the largest systematic analysis of definitive PVN undertaken thus far. In a retrospective fashion, clinical data were collected from 192 patients and correlated with morphologic findings from index biopsies at the time of initial PVN diagnosis. Histologic features were centrally scored according to Banff guidelines, including additional semiquantitative histologic assessment of intrarenal polyomavirus replication/load levels. In-depth statistical analyses, including mixed effects repeated measures models and logistic regression, revealed two independent histologic variables to be most significantly associated with clinical presentation: intrarenal polyomavirus load levels and Banff interstitial fibrosis ci scores. These two statistically determined histologic variables formed the basis for the definition of three PVN classes that correlated strongest with three clinical parameters: presentation at time of index biopsy, serum creatinine levels/renal function over 24 months of follow-up, and graft failure. The PVN classes 1–3 as described here can easily be recognized in routine renal biopsy specimens. We recommend using this morphologic PVN classification scheme for diagnostic communication, especially at the time of index diagnosis, and in scientific studies to improve comparative data analysis.

scholarly journals Monitoring and Morphologic Classification of Pediatric Cataract Using Slit-Lamp-Adapted Photography

2017 ◽  
Vol 6 (6) ◽  
pp. 2 ◽  
Author(s):  
Erping Long ◽  
Zhuoling Lin ◽  
Jingjing Chen ◽  
Zhenzhen Liu ◽  
Qianzhong Cao ◽  
...  
Keyword(s):  
Slit Lamp ◽  
Pediatric Cataract ◽  
Morphologic Classification

I—Nigrosin-Eosin Staining of Rabbit Spermatozoa and the Fertility of Semen

1958 ◽  
Vol 67 (1) ◽  
pp. 1-31 ◽  
Author(s):  
R. A. Beatty
Keyword(s):  
Birth Rate ◽  
Staining Method ◽  
Semen Quality ◽  
Domestic Animals ◽  
Biological Factors ◽  
Morphological Classification ◽  
Sources Of Variation ◽  
Highly Correlated ◽  
Rabbit Spermatozoa

SynopsisA standard nigrosin-eosin staining method in current use has been said to differentiate between live (unstained) and dead (stained) spermatozoa. In the present work, the method is applied to rabbit semen. The general results are comparable with those already obtained in the larger domestic animals. The effects of several technical and biological factors on staining capacity are evaluated, and the validity of the method is examined experimentally. Some statistical aspects are presented. The percentage of stained spermatozoa varies widely between rabbit males. To a large extent, any particular male preserves a characteristic percentage from one ejaculate to another. The percentage of stained spermatozoa is inversely related to the fertility of males as measured by the birth rate after natural mating.Concurrently, an investigation was made of the incidence of a class of morphologically abnormal spermatozoa seen in the preparations. The classifications of spermatozoa by morphology and by staining capacity are highly correlated. For assessing semen quality, the morphological classification is considered to be as useful as the classification by staining capacity, and perhaps more useful.Nigrosin-eosin staining of rabbit spermatozoa is discussed; firstly, in comparison with other animals and in relation to sources of variation; secondly, as a means of differentiating between live and dead spermatozoa; thirdly, as a means of assessing the fertility of semen; and fourthly, in relation to the alternative classification of spermatozoa by abnormal morphology.

Morphologic Classification of Congenital Short Pancreas on Multidetector Computed Tomography

2013 ◽  
Vol 37 (5) ◽  
pp. 797-804 ◽  
Author(s):  
Hyun Suk Cho ◽  
Ji Young Woo ◽  
Hye-Suk Hong ◽  
Yoo Na Kim ◽  
Ik Yang ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Multidetector Computed Tomography ◽  
Morphologic Classification ◽  
Short Pancreas
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