Success rates following intracytoplasmic sperm injection are improved by using ZIFT vs IVF for embryo transfer

1996 ◽  
Vol 13 (10) ◽  
pp. 782-785 ◽  
Author(s):  
Jeffrey Boldt ◽  
Patricia Schnarr ◽  
Anne Ajamie ◽  
Julie Ketner ◽  
Leo Bonaventura ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Intracytoplasmic Sperm Injection ◽  
Success Rates

Effects of coasting on the outcome of intracytoplasmic sperm injection-embryo transfer cycles

2004 ◽  
Vol 44 (4) ◽  
pp. 298-301 ◽  
Author(s):  
Erdal AKTAN ◽  
Kaan BOZKURT ◽  
Dilek OZER ◽  
Sait YUCEBILGIN ◽  
Nedim KARADADAS ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Intracytoplasmic Sperm Injection

Embryos from "0PN" Zygotes May Be Most Likely 2PN Embryo in L-R-ICSI

2020 ◽  
Author(s):  
Caixia Liu ◽  
Dong Han ◽  
Jianguo Chen ◽  
Ying Liu ◽  
Dong Li ◽  
...  
Keyword(s):  
Case Report ◽  
Embryo Transfer ◽  
Intracytoplasmic Sperm Injection ◽  
Healthy Children ◽  
Healthy Infants ◽  
Infertile Couples

Abstract Although some zero pronuclei (0PN) zygotes cleave and develop into good embryos, they are usually discarded because they lack two distinct pronuclei (2PN). In this case report, we followed four couples to determine whether 0PN embryo could be used in late rescue intracytoplasmic sperm injection (L-R-ICSI). Here we report five healthy infants, including one set of twins, derived from "0PN" embryos from four frozen–warmed embryo transfer (FET) cycles in L-R-ICSI between 2015 and 2017. Of nine infants born from L-R-ICSI cycles in our center, five were "0PN" embryos, and all remain healthy. Embryos from "0PNs" such as 2PN can develop into healthy babies using L-R-ICSI. This finding suggests that embryos from "0PNs" in L-R-ICSI may be different from traditional 0PN gametes and more likely to originate from 2PN gametes and they may be used in those infertile couples who lack 2PN embryos, instead of discarding them. The use of "0PN" embryos increases the cycles reaching embryo transfer, allowing some infertile couples to have healthy children and to avoid mental anguish and wasted time and money.

Detecting oestrus in maiden beef heifers using androgenised cows

1988 ◽  
Vol 1988 ◽  
pp. 136-136
Author(s):  
P. J. Broadbent ◽  
D. C. Macdonald ◽  
G. Paterson ◽  
D. F. Dolman ◽  
G. Wilson
Keyword(s):  
Embryo Transfer ◽  
Artificial Insemination ◽  
Success Rates ◽  
Beef Heifers ◽  
Formal Evaluation ◽  
Subsequent Trial

The ability to detect oestrus in cattle is important where artificial insemination, superovulation or embryo transfer techniques are being used. Even where oestrus is synchronised or controlled, knowlege of the occurrence of oestrus can increase success rates and reduce costs. A testosterone treated female fitted with a chinball marker (Signoret, 1975; Kiser, Britt and Ritchie, 1977) has been shown to be a useful aid to detection of oestrus. A preliminary experience using such females in conjunction with maiden heifers supported these claims and a subsequent trial provided a more formal evaluation of the technique.

Freeze-only versus fresh embryo transfer in a multicenter matched cohort study: contribution of progesterone and maternal age to success rates

2017 ◽  
Vol 108 (2) ◽  
pp. 254-261.e4 ◽  
Author(s):  
Ange Wang ◽  
Anthony Santistevan ◽  
Karen Hunter Cohn ◽  
Alan Copperman ◽  
John Nulsen ◽  
...  
Keyword(s):  
Cohort Study ◽  
Embryo Transfer ◽  
Maternal Age ◽  
Matched Cohort ◽  
Success Rates ◽  
Fresh Embryo Transfer ◽  
Matched Cohort Study

133 NONINVASIVE ANALYSIS OF EMBRYO METABOLITES USING NUCLEAR MAGNETIC RESONANCE

2015 ◽  
Vol 27 (1) ◽  
pp. 158
Author(s):  
M. Rubessa ◽  
K. K. Herzog ◽  
A. Ambrosi ◽  
J. W. Stewart ◽  
K. M. Polkoff ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Deuterium Oxide ◽  
Internal Standard ◽  
Single Embryo Transfer ◽  
Success Rates ◽  
Noninvasive Technique ◽  
Parthenogenetic Activation ◽  
Morphological Criteria ◽  
Parthenogenetic Embryos

Wide-spread use of IVF has significantly increased the number of multiple births (Janvier et al. 2011 J. Pediatr. 159, 409–413). A potential solution to this problem is to develop improved methods for embryo selection to permit single-embryo transfer. Identification of a noninvasive technique to assess embryo implantation potential in assisted reproduction would greatly increase success rates and lead to more efficient single-embryo transfer. The aim of this study was to assess whether there are metabolic differences among embryos produced by IVF and embryos obtained by parthenogenetic activation. Matured bovine cumulus-oocyte complexes were fertilized in vitro according to our standard procedures (Rubessa et al. 2011 Theriogenology 76, 1347–1355). Presumptive zygotes were placed in individual drops of 50 μL of SOF. Zygotes were incubated in a humidified mixture of 5% CO2, 6% O2, and 88% N2 in air at 39°C. For the parthenogenetic group, the oocytes were activated by 5 μM ionomycin in M199 + 10% FCS for 5 min, and incubation in 2 mM 6-DMAP in M199 + 10% FCS for 4 h. After 48 h, the zygotes were placed into WOW culture and the drops collected in tubes. The embryos were scored for quality on the basis of morphological criteria. Samples of media (40 μL) were added to 660 μL of a stock solution prepared by dissolving 5.0 mg of sodium 3-(trimethylsilyl)-2,2′,3,3′-tetradeuteropropionate in 50 mL of deuterium oxide. The sodium 3-(trimethylsilyl)-2,2′,3,3′-tetradeuteropropionate acted both as a chemical shift reference and as an internal standard for the purposes of quantitation. Samples were analysed on a Varian VNS-750 NB (750 MHz) spectrometer (Agilent Technologies, Santa Clara, CA, USA). Data were statistically analysed with ANOVA using the Generalized Linear Model (GLM) procedure (SAS, version 9, 1999, SAS Institute Inc., Cary, NC, USA), where the independent variable was the sample (IVF or parthenogenetic embryos and control media without embryos). Tukey's post-hoc test was used to perform multiple comparisons. The P-level was set at 0.05. All data were expressed as quadratic means with standard error of the means. The results, reported in Table 1, show that there were no statistical differences between embryo metabolites with IVF or parthenogenetic activation when we evaluated lactate, formate, myo-inositol, and pyruvate. However, we can see that there are differences when we focused on acetate and citrate. Parthenogenetic embryos produced more citrate than IVF embryos. It is well known that the Krebs cycle produces one molecule of acetate for each molecule of citrate. The present results support that as well with the concentration of acetate being greater in parthenogenetic than in the IVF embryos. These results are a first step in identifying noninvasive, quantitative parameters that indicate which embryos may be the most viable before transfer. Table 1.Results (least squares means ± s.e.)

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