Systematics of yeast species in the Candida parapsilosis group

J. W. Fell; Sally A. Meyer

doi:10.1007/bf02049795

Isolation, identification and diversity of oleaginous yeasts from Kuching, Sarawak, Malaysia

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d190412 ◽

2018 ◽

Vol 19 (4) ◽

pp. 1266-1272 ◽

Cited By ~ 1

Author(s):

MICKY VINCENT ◽

HUANG CHAI HUNG ◽

PATRICIA ROWENA MARK BARAN ◽

AFIZUL SAFWAN AZAHARI ◽

DAYANG SALWANI AWANG ADENI

Keyword(s):

Candida Parapsilosis ◽

Yeast Species ◽

Lipid Production ◽

Industrial Applications ◽

Oleaginous Yeasts ◽

Intracellular Lipids ◽

Pichia Manshurica ◽

Sudan Iv ◽

Scientific Technical ◽

Polymerase Chain

Vincent M, Hung MC, Baran PRM, Azahari AS, Adeni DSA. 2018. Isolation, identification and diversity of oleaginous yeastsfrom Kuching, Sarawak, Malaysia. Biodiversitas 19: 1266-1272. The present study was performed to isolate, identify and determine thediversity of oleaginous yeasts from various sources in Kuching, Sarawak (Malaysia). Microscopic observations via light and scanningelectron microscope (SEM) indicated that the yeast isolates were in sizes ranging from 2-3 μm in width and 4-8 μm in length, typical ofmost unicellular ascomycotic fungi. Polymerase Chain Reaction (PCR) and molecular identification performed on the yeast isolates,targeting the D1/D2 region of the 26S rDNA, identified 6 yeast species from the 21 isolates, namely Pichia manshurica (5/21), Candidakrusei (8/21), Candida parapsilosis (1/21), Pichia guilliermondii (2/21), Clavispora lusitaniae (1/21) and Kluyveromyces marxianus(4/21). All 21 yeast isolates accumulated intracellular lipids when grown in nitrogen-limited medium, as tested via Sudan IV staining.The present study is the first to document the production of lipids bodies in C. krusei, C. parapsilosis, and C. lusitaniae. Furtherinvestigations to assess the growth kinetics, lipid production efficiencies and lipids profiles of these oleaginous yeasts may provideinsights into the possible utilization of these isolates for a variety of scientific, technical and industrial applications.

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Dishwashers as an Extreme Environment of Potentially Pathogenic Yeast Species

Pathogens ◽

10.3390/pathogens10040446 ◽

2021 ◽

Vol 10 (4) ◽

pp. 446

Author(s):

Kamila Kulesza ◽

Anna Biedunkiewicz ◽

Karolina Nowacka ◽

Maria Dynowska ◽

Monika Urbaniak ◽

...

Keyword(s):

Ecological Niche ◽

Candida Parapsilosis ◽

Yeast Species ◽

Tap Water ◽

Extreme Environment ◽

Rhodotorula Mucilaginosa ◽

Pathogenic Yeast ◽

Exophiala Dermatitidis ◽

Yeast Strains ◽

Standard Procedures

The study aimed to compare the yeast species diversity in the specific environment of dishwashers, taking into account the potential risk for users. Yeasts were isolated from ten dishwashers and from tap water supplied to the appliances. Samples were collected for mycological analyses at the beginning of each month, from February to May 2016. Four dishwasher sites (rubber seals, detergent dispensers, sprinklers, and water drains) were analyzed. The microfungi were identified by the standard procedures applied in mycological diagnostics. To confirm species identification, molecular analysis was performed based on the sequences of the D1/D2 region. The presence of microfungi was detected in 70% of the investigated appliances. Rubber seals, detergent dispensers, and water drains were the most frequently colonized elements. Thirty-five yeast strains were isolated in this study, of which twenty-seven were obtained from dishwashers and eight from tap water. The strains belonged to six genera and six species (Candida parapsilosis, Clavispora lusitaniae, Dipodascus capitatus, Exophiala dermatitidis, Meyerozyma guilliermondii, and Rhodotorula mucilaginosa). Most of the strains came from rubber seals. In this way, it was demonstrated that the dishwashers’ condition is sufficient as an ecological niche for microfungi.

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Polymorphic centromere locations in the pathogenic yeast Candida parapsilosis

10.1101/2020.04.09.034512 ◽

2020 ◽

Cited By ~ 1

Author(s):

Mihaela Ola ◽

Caoimhe E. O’Brien ◽

Aisling Y. Coughlan ◽

Qinxi Ma ◽

Paul D. Donovan ◽

...

Keyword(s):

Dna Sequence ◽

Inverted Repeat ◽

Candida Parapsilosis ◽

Yeast Species ◽

Structural Features ◽

Genomic Rearrangements ◽

Pathogenic Yeast ◽

Chromosomal Site ◽

Genetically Determined

ABSTRACTCentromeres pose an evolutionary paradox: strongly conserved in function, but rapidly changing in sequence and structure. However, in the absence of damage, centromere locations are usually conserved within a species. We report here that isolates of the pathogenic yeast species Candida parapsilosis exhibit within-species polymorphism for the location of centromeres on two of its eight chromosomes. Its old centromeres have an inverted-repeat (IR) structure, whereas its new centromeres have no obvious structural features, but are located within 30 kb of the old site. Centromeres can therefore move naturally from one chromosomal site to another, apparently spontaneously and in the absence of any significant changes in DNA sequence. Our observations are consistent with a model where all centromeres are genetically determined, such as by the presence of short or long IRs, or the ability to form cruciforms. We also find that centromeres have been hotspots for genomic rearrangements in the C. parapsilosis clade.

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