Replacement of native copper in superoxide dismutase with copper-64 without much loss in its enzymatic activity

1992 ◽  
Vol 159 (1) ◽  
pp. 9-12 ◽  
Author(s):  
S. Iqbal ◽  
A. Naqvi ◽  
M. H. Rizvi
Keyword(s):  
Superoxide Dismutase ◽  
Enzymatic Activity ◽  
Native Copper

scholarly journals Antioxidant Enzyme Activities of some Brassica Species

2017 ◽  
Vol 74 (2) ◽  
pp. 191
Author(s):  
Rodica SOARE ◽  
Maria DINU ◽  
Cristina BĂBEANU ◽  
Mihaela POPESCU
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Enzymatic Activity ◽  
Brassica Oleracea ◽  
Radical Cation ◽  
Colorimetric Method ◽  
Antioxidant Enzyme Activities ◽  
Red Cabbage ◽  
Soluble Peroxidase ◽  
Brassica Oleracea L

This paper set out to comparatively study five species: white cabbage (Brassica oleracea L. var. capitata alba Alef.), red cabbage (Brassica oleracea L. var. capitata f. rubra Alef.), Kale (Brassica oleracea L. var. Acephala), cauliflower (Brassica oleracea var. botrytis) and broccoli (Brassica oleracea var. cymosa) in order to identify those with high enzymatic and antioxidant activities. The enzymatic activity of superoxide dismutase (SOD), catalase (CAT) and soluble peroxidase (POX) as well as the antioxidant activity against 2.2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical cation were determined. Total superoxide dismutase activity was measured spectrophotometrically based on inhibition in the photochemical reduction of nitroblue tetrazolium. Total soluble peroxidase was assayed by measuring the increase in A436 due to the guaiacol oxidation and the catalase activity was assayed through the colorimetric method. The capacity of extracts to scavenge the ABTS radical cation was assessed colorimetric using Trolox as a standard. The obtained results show that studied enzymatic activities and the antioxidant activity against ABTS vary depending on the analyzed species. So, among the studied Brassicaceae species, it emphasize red cabbage with the highest enzymatic activity (CAT 22.54 mM H2O2/min/g and POX 187.2 mM ΔA/1min/1g f.w.) and kale with highest antioxidant activity, of 767 μmol TE/100g f.w. The results of this study recommendintroducing the studied varieties in diet due to the rich antioxidant properties.

Oxidative Damage of Brain Induced by Nano-CdSeS in Mice

2013 ◽  
Vol 669 ◽  
pp. 316-324 ◽  
Author(s):  
Shang Yue Yang ◽  
Ran Feng Ye ◽  
Wen Jun Cai ◽  
Xiao Ling Xiang ◽  
Xu Yang
Keyword(s):  
Superoxide Dismutase ◽  
Oxidative Damage ◽  
Enzymatic Activity ◽  
Blood Brain Barrier ◽  
Saline Solution ◽  
Tail Moment ◽  
Sod Activity ◽  
Damage Degree ◽  
Mda Content ◽  
Different Doses

In this experiment, the oxidative damage of nano-CdSeS in mice brains was performed. 20 male Kunming mice were divided into 4 groups and 3 experimental groups were exposed to different doses of nano-CdSeS (0.1 mg/mL, 0.2 mg/mL and 0.4 mg/mL) by intravenous administration while the control used saline solution instead. Three days later, the enzymatic activity of superoxide dismutase (SOD), the content of malondialdehyde (MDA) and the damage degree of DNA were determined to assess the oxidative damage in brain tissues. Our results showed that in the experimental groups, SOD activity was inhibited and MDA content was increased as the doses rising, at the same time, tail moment and tail DNA% increased significantly when comparing with the control. And these results exhibited a certain doses-dependency relations. From results above, it demonstrated that oxidative damage of brain induced by nano-CdSeS which enter into blood–brain barrier in mice.

scholarly journals Accumulation of organic solutes and enzymatic activity in cut roses (Rosaceae) cultivated with physiological effect products in the Sub- Middle São Francisco River Valley

2019 ◽  
Vol 49 (12) ◽  
Author(s):  
Maria de Lourdes Neres da Silva ◽  
Mariana Correia Santos ◽  
Mayara Suzanne de Melo Barbosa ◽  
João Henrique Ferreira Sabino ◽  
Hugo Leonardo Coelho Ribeiro ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Plant Growth ◽  
Enzymatic Activity ◽  
Plant Growth Regulators ◽  
High Temperatures ◽  
Growth Regulators ◽  
Physiological Effect ◽  
River Valley ◽  
Organic Solutes ◽  
Sao Francisco River

ABSTRACT: The aim of the present study was to evaluate the accumulation of carbohydrates, protein, and proline as well as the activity of the antioxidant enzymes superoxide dismutase and catalase in the Ambiance cultivar of cut rose plants grown with the application of physiological effect products in the Sub-Middle São Francisco River Valley in Brazil. The experiment was performed under a mesh screen with 50% shading. The experimental design used randomized blocks with four repetitions and six treatments: T1) control (water); T2) boscalid; T3) pyraclostrobin; T4) boscalid + pyraclostrobin (T2 + T3); T5) fluxapyroxad + pyraclostrobin; T6) plant growth regulators 4-(indol-3-yl) butyric acid (IBA) + gibberellic acid (GA3) + kinetin; these treatments were applied every 15 days throughout the crop cycle. To determine the accumulation of solutes and enzymatic activity, 8 leaves was collected every 48 h. Leaves were immediately immersed in liquid nitrogen and frozen until further analysis in the laboratory. Results showed that the product combinations boscalid + pyraclostrobin and fluxapyroxad + pyraclostrobin as well as the plant growth regulators were the treatments with the most consistent responses throughout the evaluated cycle, providing a greater accumulation of solutes in rose leaves, as an osmotic adjustment strategy against stress from high temperatures, particularly when proline accumulation is observed. With regard to enzymatic activity, plant regulators showed more consistent results when compared with other treatments, increasing both superoxide dismutase and catalase activity. The marked accumulation of organic solutes and the high enzymatic activity, particularly of catalase, indicated that rose plants use such mechanisms as a defense against the region’s high temperatures.

Antibodies to enzymes of antioxidant system as a pathogenetic component of anemia in rheumatoid arthritis

2019 ◽  
pp. 96-100
Author(s):  
И.П. Гонтарь ◽  
О.И. Емельянова ◽  
О.А. Русанова ◽  
Л.А. Маслакова ◽  
И.А. Зборовская ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Magnetic Material ◽  
Superoxide Dismutase ◽  
Enzymatic Activity ◽  
Blood Serum ◽  
Glutathione Reductase ◽  
Protein Concentration ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antibody Concentration ◽  
Glutaric Aldehyde

Цель исследования - изучение антителообразования к супероксиддисмутазе, глутатионредуктазе, каталазе у больных ревматоидным артритом и выяснение их роли в патогенезе сопутствующей анемии. Методика. Проведено обследование 104 больных ревматоидным артритом с различной степенью активности процесса, формой и характером течения. Диагноз ревматоидного артрита ставился на основании клинико-лабораторного и инструментального обследования больных согласно системе диагностических критериев Американской ревматологической ассоциации, предложенной в 2010 году (ACR / EULAR). Выраженность анемии оценивали по уровню гемоглобина и количеству эритроцитов. Определение уровня антител проводили методом непрямого иммуноферментного анализа [ELISA тест] с использованием иммобилизированных магнитосорбентов, представляющих собой полиакриламидные гранулы, содержащие магнитный материал и перечисленные ферменты в качестве антигена. В качестве антигенов использовались коммерческие отечественные препараты: супероксиддисмутаза из эритроцитов человека (активность 30 Ед/мг), в исследованиях использовали фермент в рабочем разведении по белку - 100 мкг/мл, глутатионредуктаза (активность 340 Ед/мг) - 200 мг/мл по белку, каталаза (активность 380 Ед/мг) - 1,4 мг/мл по белку. Учитывая достаточную относительную молекулярную массу глутатионредуктазы и каталазы, иммобилизацию проводили методом эмульсионной полимеризации в потоке газообразного азота с включением магнитного материала. В связи с небольшой относительной молекулярной массой эритроцитарной фиксации супероксиддисмутазы иммобилизацию фермента проводили путем пришивки его молекулы глютаровым альдегидом к инертной полиакриламидной грануле, содержащей магнитный материал. «Чистые» антитела к ферментам получали с помощью соответствующего антигенного иммуносорбента. Источником специфических иммуноглобулинов служили сыворотки больных ревматоидным артритом с заранее определенным высоким титром антител (экстинция>0,2). После инкубации антигенного иммуносорбента и растворимой формы фермента с полученными антителами был проведен анализ изменения активности энзима. Статистическую обработку результатов проводили с использованием программных пакетов Statistica 6.0, Excel 5.0, Statgraphics 3.0, SPSS 12.0. Результаты. Выявлена зависимость между уровнем антител к супероксиддисмутазе, глутатионредуктазе и каталазе и активностью, течением и формой болезни. Полученные результаты свидетельствуют о повышении антителогенеза к этим ферментам по мере активизации патологического процесса. Выявленное снижение активности энзимов, связанное с выработкой антител к ним, происходит, очевидно, вследствие блокирования специфическими иммуноглобулинами активного центра фермента, являющегося одновременно и антигенной детерминантой. Об участии антител к ферментам в патогенезе анемии у больных ревматоидным артритом свидетельствует обратная корреляция между содержанием антител и уровнем гемоглобина. У больных ревматоидным артритом с умеренно выраженной анемией содержание антител было значимо выше, чем у пациентов без анемии, но ниже, чем у пациентов с тяжелой формой анемии. Заключение. Антитела к супероксиддисмутазе, глутатионредуктазе и каталазе являются одним из патогенетических факторов развития анемии и могут служить критерием тяжести заболевания. The aim was to study formation of antibodies to superoxide dismutase (SOD), glutathione reductase (GR), and catalase (CAT) in patients with rheumatoid arthritis (RA) and to elucidate the role of the antibodies in development of anemia. Methods. The antibodies were determined by enzyme-linked immunosorbent assay (ELISA) using immobilized magnetic sorbents, polyacrylamide granules containing a magnetic substance and the enzymes (SOD, GR, CAT) as antigens. Antibody concentration was expressed in absorbance units. Rheumatoid arthritis was diagnosed based on clinical and instrumental evaluation of patients according to the criteria of the American College of Rheumatology (ACR/EULAR, 2010). Statistical analysis was performed using variation statistics, and results were expressed as mean±SEM. Central tendencies were compared using the Student’s test. Differences were considered statistically significant at p<0.05. ELISA was performed on blood serum from 104 rheumatoid arthritis patients with different disease activity. The following commercial reagents (Russia) were used as antigens: superoxide dismutase from human RBCs (30 U/mg), which was used in the study in a working protein dilution of 100 mcg/ml; glutathione reductase (340 U/mg) which was used in the study at a protein concentration of 200 mg/ml; and catalase (380 U/mg), which was used in the study at a protein concentration of 1.4 mg/ml. Since the relative molecular weights of glutathione reductase and catalase were sufficient, immobilization was performed by emulsion polymerization in a flow of gaseous nitrogen including a magnetic material. Due to a low relative molecular weight of SOD from RBCs this enzyme was immobilized by coupling to an inert polyacrylamide granule containing a magnetic material, using glutaric aldehyde. «Pure» antibodies to the enzymes were obtained using a respective antigen immunosorbent. Specific immunoglobulins were obtained from blood serum of rheumatoid arthritis patients with a known high antibody titer (extinction > 0.2). Enzymatic activity was analyzed following incubation of the antigen immunosorbent and soluble enzyme with the obtained antibodies. Results. Production of antibodies to the studied enzymes increased with increasing severity of the disease. The decrease in enzymatic activity associated with production of respective enzyme antibodies is apparently due to inhibition by specific immunoglobulins of the active center, which is also the antigenic determinant. Conclusion. Antibodies to SOD, GR, and CAT are pathogenetic factors in the development of anemia; they may serve as a criterion for disease severity.

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