The distribution of selenium and other elements in corn samples studied by NAA and a biochemical technique

1991 ◽  
Vol 151 (2) ◽  
pp. 287-292 ◽  
Author(s):  
Tian Jibing ◽  
Qian Qingfang ◽  
Chai Chifang ◽  
Wang Ke ◽  
Ou Tong
Keyword(s):  
Biochemical Technique

scholarly journals DiADeM: differential analysis via dependency modelling of chromatin interactions with robust generalized linear models

2019 ◽  
Author(s):  
Rafał Zaborowski ◽  
Bartek Wilczyński
Keyword(s):  
Generalized Linear Models ◽  
Linear Models ◽  
Standard Technique ◽  
Differential Analysis ◽  
Genomic Distance ◽  
Chromosome Conformation ◽  
Structure And Dynamics ◽  
Chromatin Interactions ◽  
Coverage Bias ◽  
Biochemical Technique

AbstractHigh throughput Chromosome Conformation Capture experiments have become the standard technique to assess the structure and dynamics of chromosomes in living cells. As any other sufficiently advanced biochemical technique, Hi-C datasets are complex and contain multiple documented biases, with the main ones being the non-uniform read coverage and the decay of contact coverage with genomic distance. Both of these effects have been studied and there are published methods that are able to normalize different Hi-C data to mitigate these biases to some extent. It is crucial that this is done properly, or otherwise the results of any comparative analysis of two or more Hi-C experiments are bound to be biased. In this paper we study both mentioned biases present in the Hi-C data and show that normalization techniques aimed at alleviating the coverage bias are at the same time exacerbating the problems with contact decay bias. We also postulate that it is possible to use generalized linear models to directly compare non-normalized data an that it is giving better results in identification of differential contacts between Hi-C matrices than using the normalized data.

scholarly journals Biochemical analysis of specific histamine HI and H2 receptors on lymphocytes

Blood ◽  
1981 ◽  
Vol 58 (1) ◽  
pp. 87-90
Author(s):  
ME Osband ◽  
EB Cohen ◽  
BR Miller ◽  
YJ Shen ◽  
L Cohen ◽  
...  
Keyword(s):  
Biochemical Analysis ◽  
Flow Cytometric Analysis ◽  
Histamine Receptor ◽  
Membrane Receptors ◽  
Lymphocyte Function ◽  
Lymphocyte Surface ◽  
Flow Cytometric ◽  
Biochemical Technique ◽  
H2 Receptors ◽  
Receptor Distribution

It is increasingly clear that histamine mediates a variety of lymphocyte functions. Further understanding of these mechanisms requires a method for the analysis of histamine membrane receptors on the lymphocyte surface. We report now a biochemical technique for the identification and quantitation of specific histamine H1 and H2 receptors of lymphocytes. The method can be performed on small numbers of formaldehyde-fixed cells. The data this assay yields, together with that resulting from the flow cytometric analysis of histamine receptor distribution (a technique we have previously described), will be a powerful tool in the study of histamine mediation of lymphocyte function.

scholarly journals Variations in serum alkaline DNase activity: a new means to assess early detection of relapse in patients treated for acute nonlymphoblastic leukemia

Blood ◽  
1989 ◽  
Vol 74 (8) ◽  
pp. 2730-2732
Author(s):  
A Economidou-Karaoglou ◽  
M Lans ◽  
H Taper ◽  
JL Michaux ◽  
M Roberfroid
Keyword(s):  
Early Detection ◽  
Clinical Symptoms ◽  
Clinical Results ◽  
Dnase Activity ◽  
Therapeutic Monitoring ◽  
Clinical Parameters ◽  
Progressive Decrease ◽  
Biochemical Technique ◽  
Acute Nonlymphoblastic Leukemia ◽  
Observation Period

Our previously published clinical results on various malignancies indicated that the variations in serum alkaline DNase activity (SADA) could be a sensitive test for therapeutic monitoring of human malignancies. In the present study, the clinical efficacy of SADA detecting relapse in 32 acute nonlymphoblastic leukemia (ANLL) patients in remission was tested. The observation period ranged from 3 to 17 months. A simple and rapid biochemical technique based on spectrophotometric measurements was used to assay SADA. Of the 32 patients, 17 remained in remission and had less than a 15% variation in SADA levels. They had no clinical symptoms of recurrence at any time. In the remaining 15 patients, after a period of stability, a progressive decrease in SADA, with variations of more than 15%, was observed without any treatment. At that time, no abnormalities of clinical parameters were detected in these patients. A recurrence of disease as evidenced by routine examinations was found relatively late after the first decrease in SADA in all 15 patients (range 1.5 to 5.5 months). These results suggest that periodic measurements of SADA during the posttherapeutic course can be used as a means to assess early detection of an eventual recurrence.

scholarly journals Thyroxine: its biosynthesis and its immunochemistry

1944 ◽  
Vol 132 (868) ◽  
pp. 223-238 ◽  
Keyword(s):  
Thyroid Hormone ◽  
Analytical Study ◽  
Direct Evidence ◽  
Early Stage ◽  
Thyroid Tissue ◽  
General Interest ◽  
Definitive Answer ◽  
Biochemical Technique ◽  
A New Technique

The suggestion that thyroxine might be formed in nature from tyrosine through the stage of diiodotyrosine was made at an early stage of the elucidation of the chemistry of thyroxine, and was made more probable when the constitution of the latter was finally determined. Over a number of years several pieces of evidence, all indirect in character, were brought forward in support of this biogenetic hypothesis which thus came to be generally accepted. Recently two lines of direct evidence have become available which seem to place the matter beyond doubt. In the first place the transformation of diiodotyrosine into thyroxine has been effected by purely chemical methods of a character which make it possible to formulate a theory of the chemistry of the process involved. Secondly, by the application of modern biochemical technique, the actual synthesis of thyroxine from diiodotyrosine has been demonstrated in surviving thyroid tissue in vitro . The latter type of experiment incidentally offers an opportunity for the analytical study of the action of substances such as thiourea which inhibit thyroid activity supposedly by interfering with the biosynthesis of the hormone. Accepting the mechanism of biosynthesis of thyroxine as being satisfactorily established we are left with two outstanding problems. Is thyroxine itself the actual circulating thyroid hormone, and if so, by what mechanism does it exercise its effect in the periphery? To the second of these questions no answer can yet be given. Evidence regarding the first is conflicting and in the attempt to obtain a definitive answer an approach has been made along a new line which raises matters of some general interest. The method is based on the theory, deduced from the known facts of immunological chemistry, that an antigen of which the determinant group is a physiologically active substance should give rise to an antiserum capable of inhibiting the characteristic activity of this substance. Application of this idea to the problem of thyroxine involved the development of a new technique for building up artificial antigenic complexes. Such a complex containing thyroxine as the determinant group has proved to be able to give rise to an antiserum which can inhibit the physiological action both of a protein containing thyroxine, such as thyroglobulin, and of thyroxine itself. The latter observation, together with extension of the experimental method to an entirely different compound, favours the hypothesis that thyroxine itself is in fact the actual circulating thyroid hormone.

scholarly journals A global map of RNA binding protein occupancy guides functional dissection of post-transcriptional regulation of the T cell transcriptome

2018 ◽  
Author(s):  
Adam J. Litterman ◽  
Wandi S. Zhu ◽  
Robin Kageyama ◽  
Wenxue Zhao ◽  
Noah Zaitlen ◽  
...  
Keyword(s):  
T Cells ◽  
Transcriptional Regulation ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Regulatory Element ◽  
Regulatory Elements ◽  
Biochemical Technique ◽  
Cell Transcriptome ◽  
The Stability ◽  
Post Transcriptional Regulation

AbstractRNA binding proteins (RBPs) mediate constitutive RNA metabolism and gene specific regulatory interactions. To identify RNA cis-regulatory elements, we developed GCLiPP, a biochemical technique for detecting RBP occupancy transcriptome-wide. GCLiPP sequence tags corresponded with known RBP binding sites, specifically correlating to abundant cytosolic RBPs. To demonstrate the utility of our occupancy profiles, we performed functional dissection of 3′ UTRs with CRISPR/Cas9 genome editing. Two RBP occupied sites in the CD69 3′ UTR destabilized the transcript of this key regulator of lymphocyte tissue egress. Comparing human Jurkat T cells and mouse primary T cells uncovered hundreds of biochemically shared peaks of GCLiPP signal across homologous regions of human and mouse 3′ UTRs, including a cis-regulatory element that governs the stability of the mRNA that encodes the proto-oncogene PIM3 in both species. Our GCLiPP datasets provide a rich resource for investigation of post-transcriptional regulation in the immune system.

Sex determination of live galjoen (Coracinus capensis Cuvier) using a biochemical technique

Aquaculture ◽  
1990 ◽  
Vol 86 (2-3) ◽  
pp. 283-289 ◽  
Author(s):  
Carl D. Van Der Lingen ◽  
Peter A. Cook
Keyword(s):  
Sex Determination ◽  
Biochemical Technique

Biochemical analysis of specific histamine HI and H2 receptors on lymphocytes

Blood ◽  
1981 ◽  
Vol 58 (1) ◽  
pp. 87-90 ◽  
Author(s):  
ME Osband ◽  
EB Cohen ◽  
BR Miller ◽  
YJ Shen ◽  
L Cohen ◽  
...  
Keyword(s):  
Biochemical Analysis ◽  
Flow Cytometric Analysis ◽  
Histamine Receptor ◽  
Membrane Receptors ◽  
Lymphocyte Function ◽  
Lymphocyte Surface ◽  
Flow Cytometric ◽  
Biochemical Technique ◽  
H2 Receptors ◽  
Receptor Distribution

Abstract It is increasingly clear that histamine mediates a variety of lymphocyte functions. Further understanding of these mechanisms requires a method for the analysis of histamine membrane receptors on the lymphocyte surface. We report now a biochemical technique for the identification and quantitation of specific histamine H1 and H2 receptors of lymphocytes. The method can be performed on small numbers of formaldehyde-fixed cells. The data this assay yields, together with that resulting from the flow cytometric analysis of histamine receptor distribution (a technique we have previously described), will be a powerful tool in the study of histamine mediation of lymphocyte function.

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