Identification of crystals of the rimino-phenazine compound B663 (lamprene: Clofazimine) in mouse spleen macrophages by thin layer chromatography and mass spectrum analysis

1975 ◽  
Vol 31 (4) ◽  
pp. 468-469 ◽  
Author(s):  
R. T. Aplin ◽  
A. C. McDougall
Keyword(s):  
Mass Spectrum ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Spectrum Analysis ◽  
Mouse Spleen ◽  
Mass Spectrum Analysis ◽  
Layer Chromatography

scholarly journals Identification of Fluoropyrogallols as New Intermediates in Biotransformation of Monofluorophenols in Rhodococcus opacus 1cp

2000 ◽  
Vol 66 (5) ◽  
pp. 2148-2153 ◽  
Author(s):  
Zoya I. Finkelstein ◽  
Boris P. Baskunov ◽  
Marelle G. Boersma ◽  
Jacques Vervoort ◽  
Eugene L. Golovlev ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Mass Spectrum ◽  
Magnetic Resonance ◽  
Thin Layer ◽  
Chemical Shifts ◽  
Rhodococcus Opacus ◽  
Whole Cells ◽  
Mass Spectrum Analysis ◽  
Rhodococcus Opacus 1Cp ◽  
Layer Chromatography

ABSTRACT The transformation of monofluorophenols by whole cells ofRhodococcus opacus 1cp was investigated, with special emphasis on the nature of hydroxylated intermediates formed. Thin-layer chromatography, mass spectrum analysis, and 19F nuclear magnetic resonance demonstrated the formation of fluorocatechol and trihydroxyfluorobenzene derivatives from each of three monofluorophenols. The 19F chemical shifts and proton-coupled splitting patterns of the fluorine resonances of the trihydroxyfluorobenzene products established that the trihydroxylated aromatic metabolites contained hydroxyl substituents on three adjacent carbon atoms. Thus, formation of 1,2,3-trihydroxy-4-fluorobenzene (4-fluoropyrogallol) from 2-fluorophenol and formation of 1,2,3-trihydroxy-5-fluorobenzene (5-fluoropyrogallol) from 3-fluorophenol and 4-fluorophenol were observed. These results indicate the involvement of fluoropyrogallols as previously unidentified metabolites in the biotransformation of monofluorophenols in R. opacus1cp.

PROGESTERONE, 20α-DIHYDROPROGRESTERONE AND 20β-DIHYDROPROGESTERONE LEVELS IN DIFFERENT COMPARTMENTS FROM THE HUMAN FOETO-PLACENTAL UNIT

1975 ◽  
Vol 80 (3) ◽  
pp. 558-568 ◽  
Author(s):  
B. Runnebaum ◽  
H. Runnebaum ◽  
I. Stöber ◽  
J. Zander
Keyword(s):  
Liquid Chromatography ◽  
Mass Spectrum ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Reductase Activity ◽  
Placental Tissue ◽  
Gas Liquid Chromatography ◽  
Wet Weight ◽  
Layer Chromatography ◽  
Foetal Blood

ABSTRACT The endogenous levels of progesterone, 20α-dihydroprogesterone and 20β-dihydroprogesterone in different foetal tissues, in placental tissue and in foetal blood during pregnancy (week 9–25) were determined by gas-liquid chromatography or by radioimmunoassay. The identification of these steroids in foetal tissues was based on the behaviour in paper and thin-layer chromatography, the formation of different derivatives, and the retention times in gas-liquid chromatography. For additional identification of progesterone and 20α-dihydroprogesterone a mass spectrum was obtained. The average concentrations of progesterone (P), 20α-dihydroprogesterone (20α-DHP) and 20β-dihydroprogesterone (20β-DHP) in foetal tissues were as follows (ng/g wet weight tissue): brain, P 119.0 ± 45.5, 20α-DHP 86.9 ± 44.7 and 20β-DHP 9.7; lungs, P 257.7 ± 195.7, 20α-DHP 60.3 ± 26.4 and 20β-DHP 7.8; liver, P 103.7 ± 84.6, 20α-DHP 95.2 ± 89.8 and 20β-DHP 17.8; adrenals, P 295.7 ± 90.0 and 20α-DHP 254.5 ± 194.6; kidneys, P 214.2 ± 155.3 and 20α-DHP 76.6 ± 43.0; intestine, P 273.2 ± 166.7, 20α-DHP 77.9 ± 51.2 and 20β-DHP 8.7; residual tissues, P 246.3 ± 178.3 and 20α-DHP 54.8 ± 44.5 The average concentrations in placental tissue and in foetal plasma (week 12–18) were as follows (ng/g wet weight tissue or ng/ml plasma): placenta, P 2619. ± 2153.6 and 20α-DHP 461.0 ± 155.9; foetal plasma, P 378.7 ± 231.8 and 20α-DHP 283.0 ± 108.9 These results indicate that some foetal organs contain a high 20α-reductase activity at least during the first half of pregnancy.

Thin-Layer Chromatography

1965 ◽  
Author(s):  
H. R. Bolliger ◽  
M. Brenner ◽  
H. Gänshirt ◽  
Helmut K. Mangold ◽  
H. Seiler ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Layer Chromatography

THE LESS-POLAR METABOLITES PRODUCED BY INCUBATION OF TESTOSTERONE-4-14C WITH RAT AND BOVINE BRAIN

1969 ◽  
Vol 61 (4) ◽  
pp. 641-648 ◽  
Author(s):  
Leon J. Sholiton ◽  
Emile E. Werk
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Paper Chromatography ◽  
Specific Activity ◽  
Bovine Brain ◽  
Polar Fraction ◽  
End Products ◽  
Polar Metabolites ◽  
Layer Chromatography ◽  
Constant Specific Activity

ABSTRACT Rat and bovine brain have been incubated with testosterone-4-14C under standard conditions. With use of paper chromatography, the extracted metabolites were noted to fall into less-polar, iso-polar, and more polar fractions. The components of the less-polar fraction were separated by acetylation and thin-layer chromatography and the major end-products identified by recrystallization to constant specific activity or constant 3H/14C ratios. Androst-4-enedione and 5α-dihydrotestosterone were formed consistently under the conditions utilized. Trace amounts of other less-polar metabolites were noted occasionally.

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