DNA repair synthesis following exposure of guinea-pig pancreatic slices to methyl-N-nitrosourethane in vitro

1975 ◽  
Vol 31 (4) ◽  
pp. 467-467 ◽  
Author(s):  
K. Hasumi ◽  
Z. M. Iqbal ◽  
A. Alarif ◽  
S. S. Epstein
Keyword(s):  
Dna Repair ◽  
Guinea Pig ◽  
Repair Synthesis ◽  
Dna Repair Synthesis

scholarly journals Small molecule inhibitors of a human recombination-associated ATPase, RAD54

2019 ◽  
Author(s):  
Kirk T. Ehmsen ◽  
Kenny K.H. Ang ◽  
William D. Wright ◽  
Julia L. Davies ◽  
Yassir Younis ◽  
...  
Keyword(s):  
Dna Repair ◽  
Small Molecule ◽  
Small Molecule Inhibitors ◽  
Homology Search ◽  
Dna Breaks ◽  
Repair Synthesis ◽  
Molecular Events ◽  
Dna Strand Exchange ◽  
Dna Repair Synthesis

ABSTRACTHomologous recombination (HR) is a principal support pathway for DNA replication and for recovery from DNA breaks and interstrand crosslinks, making it a rational target for inhibition in cancer therapy. The ATPase RAD54 functions in molecular events that promote DNA sequence-preservation during HR-mediated damage repair, including homology search, DNA strand exchange, and transition to DNA repair synthesis within a displacement loop intermediate. We developed a high-throughput biochemical screen to identify small-molecule inhibitors of human RAD54, using a phosphate detection assay to monitor RAD54 ATPase activity in the presence of double-stranded DNA (dsDNA). After filtering potential DNA intercalators and ‘frequent hitters,’ we identified two chemotypes that reproducibly inhibited RAD54 ATPase in vitro. We evaluated these chemotypes for inhibition of RAD54-dsDNA binding and cancer cell survival. A halogenated carbazole/dihydroacridine scaffold inhibited a panel of SWI2/SNF2-related ATPases but not VCP/p97, an unrelated ATPase. Small molecules that interfere with key steps in HR— such as inhibitors of RAD54—may expose DNA repair-dependent vulnerabilities in cancer cells.

2-Nitropropane induces DNA repair synthesis in rat hepatocytes in vitro and in vivo

1988 ◽  
Vol 9 (5) ◽  
pp. 811-815 ◽  
Author(s):  
U. Andrae ◽  
L. Vogl ◽  
J. Lichtmannegger ◽  
K.H. Summer
Keyword(s):  
Dna Repair ◽  
Rat Hepatocytes ◽  
Repair Synthesis ◽  
Dna Repair Synthesis

scholarly journals Reconstitution of DNA repair synthesis in vitro and the role of polymerase and helicase activities

DNA Repair ◽  
2011 ◽  
Vol 10 (6) ◽  
pp. 567-576 ◽  
Author(s):  
Marek Sebesta ◽  
Peter Burkovics ◽  
Lajos Haracska ◽  
Lumir Krejci
Keyword(s):  
Dna Repair ◽  
Repair Synthesis ◽  
Dna Repair Synthesis

O6-Methylguanine in DNA Inhibits DNA Replication and Stimulates DNA Repair Synthesis in Vitro

1994 ◽  
Vol 726 (1 DNA Damage) ◽  
pp. 340-342 ◽  
Author(s):  
S. CECCOTTI ◽  
P. MACPHERSON ◽  
P. KARRAN ◽  
M. BIGNAMI
Keyword(s):  
Dna Repair ◽  
Dna Replication ◽  
Repair Synthesis ◽  
Dna Repair Synthesis
Sign in / Sign up

Export Citation Format

Share Document