Effect of R59022, an inhibitor of diacylglycerol kinase, on IgE-mediated histamine release from human lung mast cells and basophils

1994 ◽  
Vol 43 (1-2) ◽  
pp. 1-6 ◽  
Author(s):  
Kathryn L. O'Keefe ◽  
Jane A. Warner
Keyword(s):  
Mast Cells ◽  
Histamine Release ◽  
Human Lung ◽  
Diacylglycerol Kinase ◽  
Ige Mediated

scholarly journals IgE-mediated anaphylactic degranulation of isolated human skin mast cells

Blood ◽  
1991 ◽  
Vol 77 (3) ◽  
pp. 569-578 ◽  
Author(s):  
AM Dvorak ◽  
W Massey ◽  
J Warner ◽  
S Kissell ◽  
A Kagey-Sobotka ◽  
...  
Keyword(s):  
Mast Cells ◽  
Histamine Release ◽  
Human Skin ◽  
Human Lung ◽  
Lipid Bodies ◽  
Fusion Event ◽  
Electron Microscopic ◽  
Ultrastructural Morphology ◽  
Ige Mediated

Isolated human skin mast cells (HSMC) were prepared and cultured overnight before functional and electron microscopic studies. Mast cell suspensions were examined after stimulation with anti-IgE to produce anaphylactic degranulation or examined in buffer-incubated controls. Histamine release was measured in replicate samples. Control, isolated HSMC studied by electron microscopy were well preserved and fully granulated. Although all granule patterns reported for human mast cells were found, crystal granules were the most prevalent, as is true for HSMC in situ. Individual mast cells containing both crystal and scroll granules occurred. Lipid bodies were rare, as in HSMC in situ. Control, isolated mast cells did not express granule changes associated with either piecemeal degranulation or recovery during wound healing in situ; nor were morphologic changes of anaphylactic degranulation present. Spontaneous histamine release was 0% in control samples. Anaphylactic degranulation of isolated HSMC was accompanied by 24% maximum histamine release and characteristically showed extrusion of altered, membrane-free granules through multiple pores in the plasma membrane to the exterior of the cell. Other morphologic aspects of anaphylactic degranulation, as expressed in isolated human lung mast cells, were also present. These events included granule swelling, fusion, alteration of matrix contents, degranulation channel formation, pore formation, and shedding of granules, membranes, and surface processes. The ultrastructural morphology of isolated HSMC and their IgE-mediated degranulation shows some differences from similar studies of isolated human lung mast cells and of human lung and gut mast cells in biopsy samples. These differences include crystal granules as the predominant granule pattern, minor numbers of lipid bodies, and extrusion of granules during anaphylactic degranulation as characteristic for HSMC. By contrast, isolated human lung and gut mast cells have more scroll granules and particle granules, respectively, and more lipid bodies. In isolated human lung mast cells, anaphylactic degranulation is almost exclusively an intracellular fusion event characterized by the formation of complex degranulation channels within which altered granule matrix materials solubilize. In addition to morphologic differences between mast cells of skin, lung, or gut origin, functional differences have also been reported among mast cells of these organs. The ultrastructural morphology of isolated HSMC is identical to that of skin mast cells in biopsy samples, thereby validating the usefulness of this new source of HSMC for correlative functional and morphologic studies.

scholarly journals IgE-mediated anaphylactic degranulation of isolated human skin mast cells

Blood ◽  
1991 ◽  
Vol 77 (3) ◽  
pp. 569-578 ◽  
Author(s):  
AM Dvorak ◽  
W Massey ◽  
J Warner ◽  
S Kissell ◽  
A Kagey-Sobotka ◽  
...  
Keyword(s):  
Mast Cells ◽  
Histamine Release ◽  
Human Skin ◽  
Human Lung ◽  
Lipid Bodies ◽  
Fusion Event ◽  
Electron Microscopic ◽  
Ultrastructural Morphology ◽  
Ige Mediated

Abstract Isolated human skin mast cells (HSMC) were prepared and cultured overnight before functional and electron microscopic studies. Mast cell suspensions were examined after stimulation with anti-IgE to produce anaphylactic degranulation or examined in buffer-incubated controls. Histamine release was measured in replicate samples. Control, isolated HSMC studied by electron microscopy were well preserved and fully granulated. Although all granule patterns reported for human mast cells were found, crystal granules were the most prevalent, as is true for HSMC in situ. Individual mast cells containing both crystal and scroll granules occurred. Lipid bodies were rare, as in HSMC in situ. Control, isolated mast cells did not express granule changes associated with either piecemeal degranulation or recovery during wound healing in situ; nor were morphologic changes of anaphylactic degranulation present. Spontaneous histamine release was 0% in control samples. Anaphylactic degranulation of isolated HSMC was accompanied by 24% maximum histamine release and characteristically showed extrusion of altered, membrane-free granules through multiple pores in the plasma membrane to the exterior of the cell. Other morphologic aspects of anaphylactic degranulation, as expressed in isolated human lung mast cells, were also present. These events included granule swelling, fusion, alteration of matrix contents, degranulation channel formation, pore formation, and shedding of granules, membranes, and surface processes. The ultrastructural morphology of isolated HSMC and their IgE-mediated degranulation shows some differences from similar studies of isolated human lung mast cells and of human lung and gut mast cells in biopsy samples. These differences include crystal granules as the predominant granule pattern, minor numbers of lipid bodies, and extrusion of granules during anaphylactic degranulation as characteristic for HSMC. By contrast, isolated human lung and gut mast cells have more scroll granules and particle granules, respectively, and more lipid bodies. In isolated human lung mast cells, anaphylactic degranulation is almost exclusively an intracellular fusion event characterized by the formation of complex degranulation channels within which altered granule matrix materials solubilize. In addition to morphologic differences between mast cells of skin, lung, or gut origin, functional differences have also been reported among mast cells of these organs. The ultrastructural morphology of isolated HSMC is identical to that of skin mast cells in biopsy samples, thereby validating the usefulness of this new source of HSMC for correlative functional and morphologic studies.

scholarly journals Single-cell analysis of Ca++ changes in human lung mast cells: graded vs. all-or-nothing elevations after IgE-mediated stimulation.

1989 ◽  
Vol 109 (1) ◽  
pp. 123-134 ◽  
Author(s):  
D MacGlashan
Keyword(s):  
Mast Cells ◽  
Histamine Release ◽  
Single Cell ◽  
Human Lung ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Specific Antigens ◽  
Ige Mediated ◽  
The Mean ◽  
Nm Range

Human lung mast cells were examined by digital video microscopy for changes in cytosolic free ionized calcium [( Ca++]i) after stimulation with anti-IgE antibody or specific antigens. These studies sought to determine whether the mast cell response resembled a graded or an all-or-nothing process. Preliminary experiments indicated that labeling mast cells with fura-2 did not alter their response to IgE-mediated stimulation. Subsequent experiments established that an IgE-mediated stimulus evoked an elevation of [Ca++]i from a baseline value of 85 nM to an average of 190 nM (range 60-450 nM, n = 23), with an average histamine release of 26%. There was a good correlation (Rs = 0.67) between the average net [Ca++]i change and the subsequent histamine release (regression equation: %HR = 0.189[net(Ca)-52]). [Ca++]i elevations were found to precede histamine release (t1/2 for [Ca++]i of 35 s vs. t1/2 for histamine release of 110 s). Single-cell analysis found that even for very low values of histamine release, nearly all cells demonstrated a [Ca++]i response. However, this response was markedly heterogeneous, ranging from no response to responses two to three times the mean. Comparative studies of mast cells stimulated under optimal and suboptimal conditions established that there was a graded [Ca++]i response dependent on the strength of the stimulus. An all-or-nothing reaction for the [Ca++]i response was ruled out.

Effects of Siegesbeckia pubescens on Immediate Hypersensitivity Reaction

1997 ◽  
Vol 25 (02) ◽  
pp. 163-167 ◽  
Author(s):  
Hyung Min Kim
Keyword(s):  
Mast Cells ◽  
Hypersensitivity Reaction ◽  
Histamine Release ◽  
Immunoglobulin E ◽  
Immediate Hypersensitivity ◽  
Antiallergic Activity ◽  
Peritoneal Mast Cells ◽  
Ige Mediated ◽  
Rat Peritoneal Mast Cells ◽  
Siegesbeckia Pubescens

This study was carried out to examine the effect of an aqueous extract from Siegesbeckia pubescens (Compositae) (SPAE) on immunoglobulin E (IgE)-mediated immediate hypersensitivity reaction. Forty-eight hours passive cutaneous anaphylaxis in rats was significantly inhibited by oral administration of SPAE (100 μg/g). It also inhibited histamine release from rat peritoneal mast cells induced by anti-dinitrophenyl (DNP)-IgE and DNP-human serum albumin. The data indicate that SPAE has antiallergic activity, and that its action may be due to inhibition of histamine release from mast cells.

Aqueous Extract of Mosla chinensis Inhibits Mast Cell-Mediated Allergic Inflammation

2012 ◽  
Vol 40 (06) ◽  
pp. 1257-1270 ◽  
Author(s):  
Hui-Hun Kim ◽  
Jin-Su Yoo ◽  
Tae-Yong Shin ◽  
Sang-Hyun Kim
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Histamine Release ◽  
Aqueous Extract ◽  
Proinflammatory Cytokines ◽  
Immunoglobulin E ◽  
Inflammatory Diseases ◽  
Allergic Inflammation ◽  
Inhibitory Effect ◽  
Ige Mediated

Allergic inflammatory diseases such as food allergy, asthma, sinusitis, and atopic dermatitis are increasing worldwide. In this study, we investigated the effects of aqueous extract of Mosla chinensis Max. (AMC) on mast cell-mediated allergic inflammation and studied the possible mechanism of this action. AMC inhibited compound 48/80-induced systemic and immunoglobulin E (IgE)-mediated local anaphylaxis. AMC reduced intracellular calcium levels and downstream histamine release from rat peritoneal mast cells activated by compound 48/80 or IgE. In addition, AMC decreased gene expression and secretion of proinflammatory cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-6 and IL-8 in human mast cells. The inhibitory effect of AMC on cytokine expression was nuclear factor (NF)-κB dependent. Our results indicate that AMC inhibits mast cell-mediated allergic inflammatory reaction by suppressing histamine release and expression of proinflammatory cytokines and the involvement of calcium and NF-κB in these effects. AMC might be a possible therapeutic candidate for allergic inflammatory disorders.

scholarly journals Prostaglandin D2 Inhibits IgE-Mediated Scratching by Suppressing Histamine Release From Mast Cells

2005 ◽  
Vol 98 (1) ◽  
pp. 90-93 ◽  
Author(s):  
Yuki Hashimoto ◽  
Iwao Arai ◽  
Makoto Tanaka ◽  
Shiro Nakaike
Keyword(s):  
Mast Cells ◽  
Histamine Release ◽  
Prostaglandin D2 ◽  
Ige Mediated

scholarly journals High Rate of IgE-Mediated Histamine Release from Rat Mesenteric Mast Cells.

2001 ◽  
Vol 50 (2) ◽  
pp. 187-190 ◽  
Author(s):  
Naoki INAGAKI ◽  
Hirokazu KAWASAKI ◽  
Hiroichi NAGAI
Keyword(s):  
Mast Cells ◽  
Histamine Release ◽  
High Rate ◽  
Ige Mediated
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