Effects of immunoglobulin and gamma-interferon on the production of tumour necrosis factor-α and interleukin-1β by peripheral blood monocytes in the acute phase of Kawasaki disease

1996 ◽  
Vol 155 (4) ◽  
pp. 291-296 ◽  
Author(s):  
Hiroyuki Suzuki ◽  
Shigeru Uemura ◽  
Shigenobu Tone ◽  
Tadashi Iizuka ◽  
Michio Koike ◽  
...  
Keyword(s):  
Kawasaki Disease ◽  
Tumour Necrosis Factor ◽  
Acute Phase ◽  
Peripheral Blood ◽  
Tumour Necrosis ◽  
Tumour Necrosis Factor Α ◽  
Blood Monocytes ◽  
Peripheral Blood Monocytes ◽  
Factor Α ◽  
Necrosis Factor

scholarly journals Role of soluble gp130 in the tumour necrosis factor-α expression and its production by peripheral blood mononuclear cells

2003 ◽  
Vol 12 (6) ◽  
pp. 355-359
Author(s):  
E. Jablonskaca ◽  
W. Puzewska ◽  
M. Marcinczyk ◽  
J. Jablonski
Keyword(s):  
Tumour Necrosis Factor ◽  
Interleukin 6 ◽  
Peripheral Blood ◽  
Tumour Necrosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tumour Necrosis Factor Α ◽  
Tnf Α ◽  
Factor Α ◽  
Necrosis Factor ◽  
Soluble Gp130

Background:In our previous study we found that rhsIL-6R, along with recombinant human interleukin-6, plays a regulatory role in the immune response by modulating the tumour necrosis factor-α (TNF-α) expression and its production by peripheral blood mononuclearcells (PBMC). We also suggested that sIL-6R with IL-6 secreted by human PMN (neutrophils) influenced the TNF-α expression and its production by autologous PBMC.Aims:Since soluble gp130 (sgp130) is a natural inhibitor for sIL-6R/interleukin-6 responses, in the present study we estimated an effect of exogenous recombinant human sgp130 and sgp130 secreted by PMN on the TNF-α expression and its production by PBMC.Methods:Cells were isolated from whole blood of healthy persons. The PMN were cultured in 96-well plates for 1 h at 37°C in a humidified incubator with 5% CO2. After the incubation, the culture supernatant of PMN was removed and added to the PBMC. PBMC were incubated for 1 h at 37°C in the same conditions. Cytoplasmic protein fractions of PMN and, for comparative purpose of PBMC, were analysed for presence of sgp130 by western blotting with the use of monoclonal antibody capable of detecting this protein. In the culture supernatants of PMN we examined the concentrations of sgp130 by human enzyme-linked immunosorbent assay. TNF-α was measured at the protein levels as well as the mRNA levels.Results and conclusions:The present results revealed that exogenous recombinant human sgp130 modulates the TNF-α expression and production by PBMC. In contrast, we did not find any effect of sgp130 secreted by PMN on the TNF-α expression and its production by autologous PBMC.

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