Fusaric acid: phytotoxicity and in vitro production by Fusarium oxysporum f.sp. lilii, the causal agent of basal rot in lilies

1992 ◽  
Vol 98 (2) ◽  
pp. 107-115 ◽  
Author(s):  
H. J. M. Löffler ◽  
J. R. Mouris
Keyword(s):  
Fusarium Oxysporum ◽  
Fusaric Acid ◽  
Causal Agent ◽  
In Vitro Production ◽  
Basal Rot ◽  
Vitro Production

scholarly journals Methanolic Extracts from Cultivated Mushrooms Affect the Production of Fumonisins B and Fusaric Acid by Fusarium verticillioides

Toxins ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 366 ◽  
Author(s):  
Daniel Merel ◽  
Jean-Michel Savoie ◽  
Gerardo Mata ◽  
Dulce Salmones ◽  
Carlos Ortega ◽  
...  
Keyword(s):  
Lentinula Edodes ◽  
Culture Media ◽  
Fusarium Verticillioides ◽  
Fusaric Acid ◽  
Fruiting Bodies ◽  
In Vitro Production ◽  
Methanolic Extracts ◽  
Comparative Metabolomics ◽  
Vitro Production

The maize pathogen Fusarium verticillioides and their mycotoxins cause damage to plants, animals, and human health. This work aimed to evaluate the effect of crude extracts (CEs) from Agaricus subrufescens, Lentinula edodes, and Pleurotus ostreatus fruiting bodies on in vitro production of biomass and mycotoxins by two strains of F. verticillioides. Stipes and pilei were separated before extraction for A. subrufescens and L. edodes. Comparative metabolomics and dereplication of phenolic compounds were used to analyze all CEs. Mushroom CEs did not significantly inhibit the production of mycelial biomass at concentrations of 2 mg mL−1. CEs from A. subrufescens (stipes and pilei) and L. edodes pilei inhibited the production of fumonisins B1 + B2 + B3 by 54% to 80%, whereas CE from P. ostreatus had no effect. In contrast, CE from L. edodes stipes dramatically increased the concentration of fumonisins in culture media. Fusaric acid concentration was decreased in cultures by all CEs except L. edodes stipes. Differences in phenolic composition of the extracts may explain the different effects of the CE treatments on the production of mycotoxins. The opposing activities of stipes and pilei from L. edodes offer an opportunity to search for active compounds to control the mycotoxin production by F. verticillioides.

scholarly journals In vitro production of antifungal phenolic acids by Hypomyces perniciosus, the causal agent of wet bubble disease of Agaricus bisporus

All Life ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 948-953
Author(s):  
Majida Mohammed Ali Al-Harrasi ◽  
Abdullah Mohammed Al-Sadi ◽  
Afrah Mohammed Al-Tamimi ◽  
Jamal Nasser Al-Sabahi ◽  
Rethinasamy Velazhahan
Keyword(s):  
Phenolic Acids ◽  
Agaricus Bisporus ◽  
Causal Agent ◽  
In Vitro Production ◽  
Vitro Production

In Vitro Production of Biotrophic-Like Cultures of Crinipellis perniciosa, the Causal Agent of Witches’ Broom Disease of Theobroma cacao

2006 ◽  
Vol 52 (3) ◽  
pp. 191-196 ◽  
Author(s):  
Lyndel W. Meinhardt ◽  
Cláudia de M. Bellato ◽  
Johana Rincones ◽  
Ricardo A. Azevedo ◽  
Julio C. M. Cascardo ◽  
...  
Keyword(s):  
Theobroma Cacao ◽  
Causal Agent ◽  
In Vitro Production ◽  
Crinipellis Perniciosa ◽  
Broom Disease ◽  
Vitro Production

scholarly journals Study on usability of Fusarium oxysporum Schlecht.f.sp. tulipae Apt. metabolites for screening for basal rot resistance in tulip

2013 ◽  
Vol 54 (1) ◽  
pp. 71-82 ◽  
Author(s):  
Małgorzata Podwyszyńska ◽  
Czesław Skrzypczak ◽  
Krzysztof Fatel ◽  
Lech Michalczuk
Keyword(s):  
Fusarium Oxysporum ◽  
Shoot Growth ◽  
Fusaric Acid ◽  
Resistance Breeding ◽  
Fungus Culture ◽  
Basal Rot ◽  
Culture Filtrates ◽  
Selection Of ◽  
Greenhouse Tests

The usefulness of fungus culture filtrates and fusaric acid as selecting agents for <i>Fusarium</i> resistance breeding in tulip was examined on <i>in vitro</i> cultures of shoots and embryonic calli of seven tulip genotypes differing in resistance to <i>Fusarium oxysporum</i> Schlecht. f. sp. <i>tulipae</i> Apt. (F.o.t.) and four virulent F.o.t. isolates. Fusaric acid influenced the shoot growth of all cultivars tested in a similar way, irrespectively of their greenhouse resistance to basal rot. Also, the sensitivity of calli of the cultivars studied to fusaric acid did not correspond with their resistance to F.o.t. evaluated in the greenhouse screening. The phytotoxity of F.o.t. culture filtrates did not depend on their fusaric acid contents. There was a negative correlation between cultivar's resistance to F.o.t in greenhouse tests and the sensitivity of their shoots to fungus culture filtrates in <i>in vitro</i> tests. This indicates that defence mechanism against F.o.t. in tulip tissue may have a nature of hypersensitive response. Considering the results of our study, it may be concluded that the use of fusaric acid or fungus culture filtrates for the <i>in vitro</i> selection of somaclones resistant to F.o.t. in tulip is not feasible.

THE IN VITRO PRODUCTION OF ANDROGENS BY FOLLICULAR TISSUE OF RABBITS

1964 ◽  
Vol 47 (2) ◽  
pp. 306-313 ◽  
Author(s):  
Denis Gospodarowicz
Keyword(s):  
In Vitro Production ◽  
Vitro Production

ABSTRACT Incubation in vitro of rabbit follicles in separate experiments with dehydroepiandrosterone-14C (DHEA-14C), progesterone-14C and pregnenolone-3H in the presence of FSH gave the following results: 39 % of the radioactivity of DHEA-14C is converted to androstenedione and testosterone, while only 3 % of the radioactivity of either progesterone-14C or pregnenolone-3H is found in the androgen fraction. From the ratio of testosterone to androstenedione formed from the three precursors, the results are interpreted to mean that DHEA and pregnenolone, and not progesterone, are precursors of androgens in the follicle.

Oxygenation of 18-hydroxycorticosterone as the final reaction for aldosterone biosynthesis

1984 ◽  
Vol 107 (3) ◽  
pp. 395-400 ◽  
Author(s):  
Itaru Kojima ◽  
Etsuro Ogata ◽  
Hiroshi Inano ◽  
Bun-ichi Tamaoki
Keyword(s):  
Carbon Monoxide ◽  
Hydroxysteroid Dehydrogenase ◽  
Dependent Manner ◽  
In Vitro Production ◽  
Mitochondrial Preparation ◽  
Final Reaction ◽  
Vitro Production ◽  
Dose Dependent ◽  
Cytochrome P 450

Abstract. Incubation of 18-hydroxycorticosterone with the sonicated mitochondrial preparation of bovine adrenal glomerulosa tissue leads to the production of aldosterone, as measured by radioimmunoassay. The in vitro production of aldosterone from 18-hydroxycorticosterone requires both molecular oxygen and NADPH, and is inhibited by carbon monoxide. Cytochrome P-450 inhibitors such as metyrapone, SU 8000. SU 10603, SKF 525A, amphenone B and spironolactone decrease the biosynthesis of aldosterone from 18-hydroxycorticosterone. These results support the conclusion that the final reaction in aldosterone synthesis from 18-hydroxycorticosterone is catalyzed by an oxygenase, but not by 18-hydroxysteroid dehydrogenase. By the same preparation, the production of [3H]aldosterone but not [3H]18-hydroxycorticosterone from [1,2-3H ]corticosterone is decreased in a dose-dependent manner by addition of non-radioactive 18-hydroxycorticosterone.

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