Localizing properties of anti-nervous tissue antibodies in rat cervical ganglion

1975 ◽  
Vol 31 (2) ◽  
pp. 239-241 ◽  
Author(s):  
B. D. Janković ◽  
Kosana Mitrović ◽  
J. Horvat ◽  
V. Savić
Keyword(s):  
Nervous Tissue ◽  
Cervical Ganglion

EFFECTS OF QUATERNARY BASES AND INORGANIC CATIONS ON ACETYLCHOLINE SYNTHESIS IN NERVOUS TISSUE

1967 ◽  
Vol 45 (2) ◽  
pp. 249-268 ◽  
Author(s):  
S. P. Bhatnagar ◽  
F. C. MacIntosh
Keyword(s):  
Nervous Tissue ◽  
Inhibitory Effect ◽  
Sympathetic Ganglia ◽  
Direct Inhibition ◽  
Inorganic Cations ◽  
Tetrabutyl Ammonium ◽  
Cervical Ganglion ◽  
Choline Acetylase ◽  
High Concentrations ◽  
And Storage

A number of quaternary bases were tested for their ability to inhibit the synthesis of acetylcholine (ACh) by incubated minced mouse brain. Tetramethyl-, tetraethyl-, tetrapropyl-, and tetrabutyl-ammonium were moderately active as inhibitors, the strongest being the tetraethyl base (TEA), which was 1–2% as active as the hemicholinium HC-3. Weaker activity was exhibited by hexamethonium, d-tubocurarine, and tolazoline; NH4Cl and N-methylnicotinamide were inactive at concentrations up to 10−2 M. Except in high concentrations, none of the bases significantly affected the release of ACh from the tissue into the eserinized medium. The addition of choline to the medium somewhat increased the rate of ACh formation when the bases were absent and strikingly antagonized the inhibitory action of HC-3 and TEA. TEA also resembled HC-3 in that (i) it reduced the ACh content of the preganglionically excited superior cervical ganglion of the cat, though neither stimulation alone nor the drug alone has such an effect; (ii) it killed mice by paralyzing respiration, and this effect was antagonized by choline; (iii) its inhibitory effect on ACh synthesis by intact nervous tissue was not due to direct inhibition of choline acetylase. The finding of Birks and Quastel that extracellular Na+is essential for the synthesis and storage of ACh by perfused sympathetic ganglia was confirmed for incubated brain tissue. Neither the action of HC-3 and TEA in inhibiting ACh synthesis, nor that of Na+in promoting it, has as yet been clarified.

Evidence for a Blood Ganglion Barrier in the Superior Cervical Ganglion of the Rat

1982 ◽  
Vol 40 ◽  
pp. 204-204
Author(s):  
D. M. DePace
Keyword(s):  
Blood Vessels ◽  
Superior Cervical Ganglion ◽  
Peripheral Nerves ◽  
Time Schedule ◽  
Transmission Electron ◽  
Cervical Ganglion ◽  
The Central Nervous System ◽  
Cervical Ganglia ◽  
Capillary Circulation ◽  
And Control

The majority of blood vessels in the superior cervical ganglion possess a continuous endothelium with tight junctions. These same features have been associated with the blood brain barrier of the central nervous system and peripheral nerves. These vessels may perform a barrier function between the capillary circulation and the superior cervical ganglion. The permeability of the blood vessels in the superior cervical ganglion of the rat was tested by intravenous injection of horseradish peroxidase (HRP). Three experimental groups of four animals each were given intravenous HRP (Sigma Type II) in a dosage of.08 to.15 mg/gm body weight in.5 ml of.85% saline. The animals were sacrificed at five, ten or 15 minutes following administration of the tracer. Superior cervical ganglia were quickly removed and fixed by immersion in 2.5% glutaraldehyde in Sorenson's.1M phosphate buffer, pH 7.4. Three control animals received,5ml of saline without HRP. These were sacrificed on the same time schedule. Tissues from experimental and control animals were reacted for peroxidase activity and then processed for routine transmission electron microscopy.

Association of pituitary adenoma with neuronal christoma: A TEM study

1994 ◽  
Vol 52 ◽  
pp. 232-233
Author(s):  
Eva Horvath ◽  
Kalman Kovacs ◽  
B. W. Scheithauer ◽  
R. V. Lloyd ◽  
H. S. Smyth
Keyword(s):  
Growth Hormone ◽  
Pituitary Adenoma ◽  
Pituitary Tumor ◽  
Nervous Tissue ◽  
Alternative Explanation ◽  
Growth Hormone Releasing Hormone ◽  
Cell Hyperplasia ◽  
Releasing Hormone ◽  
Secretory Neurons

The association of a pituitary adenoma with nervous tissue consisting of neuron-like cells and neuropil is a rare abnormality. In the majority of cases, the pituitary tumor is a chromophobic adenoma, accompanied by acromegaly. Histology reveals widely variable proportions of endocrine and nervous tissue in alternating or intermingled patterns. The lesion is perceived as a composite one consisting of two histogenetically distinct parts. It has been suggested that the neuronal component, morphologically similar to secretory neurons of the hypothalamus, may initiate adenoma formation by releasing stimulatory substances. Immunoreactivity for growth hormone releasing hormone (GRH) in the neuronal component of some cases supported this view, whereas other findings such as consistent lack of growth hormone (GH) cell hyperplasia in the lesions called for alternative explanation.Fifteen tumors consisting of a pituitary adenoma and a neuronal component have been collected over a 20 yr. period. Acromegaly was present in 11 patients, was equivocal in one, and absent in 3.

Estradiol-stimulated nitric oxide release in nervous tissue, vasculature, and gonads of the giant cockroachBlaberus craniifer

2004 ◽  
Vol 55 (1-4) ◽  
pp. 143-148 ◽  
Author(s):  
F. E. Nieto-Fernandez ◽  
F. Ianuzzi ◽  
Adriana Ruiz ◽  
Lilian Nodimele
Keyword(s):  
Nitric Oxide ◽  
Nervous Tissue ◽  
Nitric Oxide Release

Anabolic steroids and their effects of on neuronal density in cortical areas and hippocampus of mice

2020 ◽  
Author(s):  
B. Damião ◽  
W. C. Rossi-Junior ◽  
F. D. R. Guerra ◽  
P. P. Marques ◽  
D. A. Nogueira ◽  
...  
Keyword(s):  
Nervous Tissue ◽  
Anabolic Steroids ◽  
Hippocampal Region ◽  
Behavioral Impairment ◽  
Neuronal Density ◽  
Cortical Areas ◽  
Illicit Use ◽  
And Performance ◽  
Serial Samples ◽  
Considerable Damage

Abstract Anabolic substances have been increasingly used by bodybuilders and athletes with the goal of improving performance and aesthetics. However, this practice has caused some concern to physicians and researchers because of unknowledge of consequences that the indiscriminate and illicit use of these substances can cause. Thus, this study analyzed the effects of two commercially available anabolic steroids (AS), Winstrol Depot® (Stanozolol) and Deposteron® (Testosterone Cypionate), in the neuronal density of limbic, motor and sensory regions on the cerebral cortex and in CA1, CA2, CA3 regions of the hippocampus. A total of 60 Swiss mice were used (30 males and 30 females), separated into three groups: control and two experimental groups, which received the AAS. From each brain, homotypic and semi-serial samples were taken in frontal sections from areas established for the study. The results showed that females treated with testosterone cypionate presented a reduction in all regions tested and the ones treated with Stanozolol showed a decrease in some hippocampal areas. Regarding male animals, stanozolol led to a decrease in neuron number in one hippocampal region. These data allow us to conclude that supra-physiological doses of steroids used in this study, can cause considerable damage to nervous tissue with ultrastructural and consequently behavioral impairment. These changes could interfere with the loss of physical yield and performance of athletes and non-athletes and may cause irreparable damage to individuals making irresponsible use of anabolic steroids.

Systemic Toxic Dental Sealant-Induced Effect on Nervous Tissue in vitro

2018 ◽  
Vol 9 (4) ◽  
pp. 287-293
Author(s):  
O. A. Znachkova ◽  
Irina V. Lushnikova ◽  
Galina G. Skibo ◽  
M. Y. Antonenko ◽  
Nana V. Voitenko
Keyword(s):  
Nervous Tissue ◽  
Dental Sealant

PREDICTION OF THE EFFICIENCY OF RECONSTRUCTIVE INTERVENTIONS ON THE MAJOR NERVES OF THE FOREARM, DEPENDING ON THE DEFECT DURATION AND THE FOLLOWING REHABILITATION

2020 ◽  
pp. 77-88
Author(s):  
Дмитрий Валериевич Судаков ◽  
Олег Валериевич Судаков ◽  
Людмила Валентиновна Кретинина ◽  
Наталья Владимировна Якушева ◽  
Артём Николаевич Шевцов
Keyword(s):  
Nervous Tissue ◽  
Clinical Situation ◽  
Economic Importance ◽  
Important Data ◽  
Subsequent Period ◽  
Complete Restoration ◽  
Reconstructive Microsurgery ◽  
The Moment

Статья посвящена построению прогноза эффективности реконструктивных вмешательств на магистральных нервах предплечья в зависимости от протяженности дефекта нервной ткани и особенностей последующего периода реабилитации пациентов. Данная тематика является весьма актуальной, так как с каждым годом во всем мире наблюдается определенный рост случаев травм различного генеза магистральных нервных стволов, которые затем нередко приводят к временной нетрудоспособности и даже инвалидности пациентов. Реконструктивная микрохирургия многие десятилетия пытается решить целый ряд проблем аутотрансплантации нервных стволов и повысить ее общую эффективность. Но из-за определенных проблем связанных с финансированием, некоторые вопросы трансплантологии и реабилитации остаются нерешенными и в настоящий момент. Все это придает представленной работе важное значение не только медицинского, но и социально - экономического плана. Целью работы стала попытка построения прогноза восстановительных операций на нервной ткани, с учетом объема пораженных структур и периода реабилитации. Объектами исследования стало 180 больных, которым по той или иной причине, осуществлялась реконструктивная операция на одном из магистральных нервов предплечья. Все пациенты были разделены на 3 группы по 60 человек, в зависимости от протяженности дефекта магистрального нерва: до 4 см, от 4 до 8 см и от 8 до 12 см. Последующее разделение внутри каждой группы на подгруппы производилось в зависимости от определенного поврежденного нерва (лучевой, локтевой, срединный). В работе изучалось течение раннего послеоперационного воспалительного процесса, с определением бактериальной микрофлоры в ране. Изучались и отдаленные последствия оперативного вмешательства. Своеобразной новизной для данной тематики в целом, стало выявление последующего установления инвалидности пациентов. Кроме того, важные данные были получены и по срокам реабилитации и частичного или полного восстановления утраченных функций по срокам в зависимости от размеров восстанавливаемого дефекта и от наличия или отсутствия необходимой реабилитации. Полученные в работе данные могут представлять интерес не только для врачей хирургов и травматологов, но и для организаторов здравоохранения, позволяя производить прогнозы по выздоровлению пациентов в каждой определенной клинической ситуации The article is devoted to the construction of a forecast of the effectiveness of reconstructive interventions on the main nerves of the forearm, depending on the length of the defect in the nervous tissue and the characteristics of the subsequent period of rehabilitation of patients. This topic is very relevant, since every year all over the world there is a certain increase in cases of injuries of various origins of the main nerve trunks, which then often lead to temporary disability and even disability of patients. For many decades, reconstructive microsurgery has been trying to solve a number of problems of autotransplantation of nerve trunks and improve its overall efficiency. But due to certain problems associated with funding, some issues of transplantation and rehabilitation remain unresolved at the moment. All this gives the presented work important not only medical, but also socio - economic importance. The aim of this work was to attempt to predict restorative operations on the nervous tissue, taking into account the volume of the affected structures and the period of rehabilitation. The objects of the study were 180 patients who, for one reason or another, underwent a reconstructive operation on one of the main nerves of the forearm. All patients were divided into 3 groups of 60 people, depending on the length of the main nerve defect: up to 4 cm, from 4 to 8 cm, and from 8 to 12 cm. Subsequent division within each group into subgroups was performed depending on the specific damaged nerve ( radial, ulnar, median). The work studied the course of the early postoperative inflammatory process, with the determination of bacterial microflora in the wound. The long-term consequences of surgery were also studied. A peculiar novelty for this topic as a whole was the identification of the subsequent establishment of disability in patients. In addition, important data were obtained on the timing of rehabilitation and partial or complete restoration of lost functions in terms of timing, depending on the size of the restored defect and on the presence or absence of the necessary rehabilitation. The data obtained in this work may be of interest not only for surgeons and traumatologists, but also for healthcare organizers, allowing them to make predictions about the recovery of patients in each specific clinical situation

Standardization of in vitro nervous tissue culture for honeybee: A high specificity toxicological approach

2020 ◽  
Vol 189 ◽  
pp. 110040 ◽  
Author(s):  
Patricia Azevedo ◽  
Nicole Pavan Butolo ◽  
Luciano Delmondes de Alencar ◽  
Hellen Maria Soares-Lima ◽  
Victor Ribeiro Sales ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Nervous Tissue ◽  
High Specificity ◽  
Nervous Tissue Culture
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