Partial recovery of fluorescence intensity after irradiation-induced fading of fluorescence, and its effects on techniques of quantitative fluorescence microscopy

1984 ◽  
Vol 40 (6) ◽  
pp. 538-539 ◽  
Author(s):  
J. H. Dowson
Keyword(s):  
Fluorescence Microscopy ◽  
Fluorescence Intensity ◽  
Partial Recovery ◽  
Quantitative Fluorescence

Measurement of volume injected into individual cells by quantitative fluorescence microscopy

1989 ◽  
Vol 94 (3) ◽  
pp. 443-447
Author(s):  
G.M. Lee
Keyword(s):  
Fluorescence Microscopy ◽  
Fluorescence Intensity ◽  
Mammalian Cells ◽  
Lucifer Yellow ◽  
Fluorescence Microscope ◽  
Standard Curve ◽  
Cellular Processes ◽  
Reliable Procedure ◽  
Dextran Solution ◽  
Quantitative Fluorescence

Pressure microinjection is frequently used to introduce substances into mammalian cells, but precise quantitation of the volume injected into individual cells has been difficult. A simple and reliable procedure for determining the volume injected was developed in order to determine what intracellular concentration of AMP-PNP was necessary to inhibit specific cellular processes. The technique uses fluorescent Lucifer Yellow-labeled dextrans in the microinjection buffer and quantitative fluorescence microscopy to measure the fluorescence intensity of the injected cell. The volume injected is computed from a standard curve derived from the volume and fluorescence of spherical, microscopic droplets of Lucifer Yellow dextran solution. The droplets are ejected from a micropipet into immersion oil where they sink to rest on a siliconized coverslip. For the measurement of fluorescence, an inexpensive photomultiplier system that is attached to a fluorescence microscope is described. The potential uses of this method for other microassays are discussed.

scholarly journals Quantitative Fluorescence Microscopy on SARS-CoV-2

2021 ◽  
Vol 120 (3) ◽  
pp. 360a
Author(s):  
Rayna M. Addabbo ◽  
John Kohler ◽  
Isaac Angert ◽  
Yan Chen ◽  
Heather Hanson ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Quantitative Fluorescence

scholarly journals Quantitative Fluorescence Microscopy Reveals Higher Order Oligomerization of FGFR5

2017 ◽  
Vol 112 (3) ◽  
pp. 88a
Author(s):  
Romario Regeenes ◽  
Pamuditha Silva ◽  
Dawn M. Kilkenny ◽  
Jonathan V. Rocheleau
Keyword(s):  
Fluorescence Microscopy ◽  
Higher Order ◽  
Quantitative Fluorescence
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