Estradiol treatment reduces a cytosol androgen binding protein in male rat liver

1980 ◽  
Vol 36 (7) ◽  
pp. 877-878 ◽  
Author(s):  
N. Sato ◽  
M. Ota ◽  
S. Takahashi
Keyword(s):  
Rat Liver ◽  
Binding Protein ◽  
Male Rat ◽  
Estradiol Treatment ◽  
Androgen Binding Protein ◽  
Androgen Binding

Characterization studies of a rat hepatie cytosolic androgen-binding protein

1985 ◽  
Vol 63 (8) ◽  
pp. 952-957 ◽  
Author(s):  
G. I. Sunahara ◽  
M. J. P. Finlayson ◽  
B. L. Warren ◽  
G. D. Bellward
Keyword(s):  
Binding Protein ◽  
High Capacity ◽  
Male Rat ◽  
High Affinity ◽  
Androgen Binding Protein ◽  
Sex Dependency ◽  
Testosterone Administration ◽  
Male Specific ◽  
Androgen Binding ◽  
Estrogen Binding

A rat hepatic cytosolic [3Hjmethyltrienolone (R1881) binding protein was studied under various conditions. This protein was also compared with the male-specific high capacity – low affinity estrogen-binding protein derived from the same cytosolic fraction. Analysis of the R1881 binding protein in adult (60–85 days old) male rat liver cytosol indicated the presence of a high affinity – low capacity binding site (Kd = 0.3 nM; Bmax = 5.9 fmol/mg) and a lower affinity – higher capacity component (Kd = 10.4 nM; Bmax = 131 fmol/mg). The latter component was eliminated by addition of triamcinolone or cortisol to the assay mixture. Steroid binding to the high affinity R1881 site was specific for testosterone, dihydrotestosterone, androstenedione, and mibolerone, with a moderate specificity to cyproterone acetate, flutamide hydroxide, and estradiol. Saturation studies indicated that these steroids were binding to the same or a similar high affinity component except for flutamide hydroxide which produced nonsaturable displacement. The high affinity site had no specificity for progesterone, diethylstilbestrol, or cortisol. Like the high capacity – low affinity protein, this protein was not present in the immature, adult, or 10-day ovariectomized adult female. However, unlike the high capacity – low affinity protein, it was present in low quantities in the immature male. In addition, castration of the adult for 18 h, 4 days, or 10 days or hypophysectomy for 10–17 days did not have a significant effect on the high affinity component compared with the controls. Testosterone administration to these animals did not alter this protein binding. These studies indicate that a specific, high affinity – low capacity androgen-binding protein exists in rat hepatic cytosol. Furthermore, this protein shows age and sex dependency, but its presence is not affected by altering gonadal or hypophyseal factors in the adult male.

Purification and immunochemical characterization of the cytoplasmic androgen-binding protein of rat liver

Biochemistry ◽  
1989 ◽  
Vol 28 (4) ◽  
pp. 1732-1736 ◽  
Author(s):  
William F. Demyan ◽  
Fazlul H. Sarkar ◽  
C. V. Ramana Murty ◽  
Arun K. Roy
Keyword(s):  
Rat Liver ◽  
Binding Protein ◽  
Immunochemical Characterization ◽  
Androgen Binding Protein ◽  
Androgen Binding

Immunocytochemical Localization of Androgen-Binding Protein in the Male Rat Reproductive Tract*

Endocrinology ◽  
1981 ◽  
Vol 108 (3) ◽  
pp. 925-931 ◽  
Author(s):  
LAURI J. PELLINIEMI ◽  
MARTIN DYM ◽  
GLEN L. GUNSALUS ◽  
NEAL A. MUSTO ◽  
C. WAYNE BARDIN ◽  
...  
Keyword(s):  
Reproductive Tract ◽  
Binding Protein ◽  
Male Rat ◽  
Immunocytochemical Localization ◽  
Androgen Binding Protein ◽  
Androgen Binding

scholarly journals Estrogen Receptor β Expression and Apoptosis of Spermatocytes of Mice Overexpressing a Rat Androgen-Binding Protein Transgene1

2004 ◽  
Vol 71 (5) ◽  
pp. 1461-1468 ◽  
Author(s):  
David M. Selva ◽  
Oscar M. Tirado ◽  
Nuria Toràn ◽  
Carlos A. Suárez-Quian ◽  
Jaume Reventos ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Binding Protein ◽  
Estrogen Receptor Β ◽  
Androgen Binding Protein ◽  
Androgen Binding

scholarly journals Overexpression of Androgen-Binding Protein/Sex Hormone-Binding Globulin in Male Transgenic Mice: Tissue Distribution and Phenotypic Disorders1

1997 ◽  
Vol 56 (1) ◽  
pp. 21-32 ◽  
Author(s):  
David R. Joseph ◽  
Deborah A. O’Brien ◽  
Patrick M. Sullivan ◽  
Marzia Becchis ◽  
James K Tsuruta ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Tissue Distribution ◽  
Binding Protein ◽  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Hormone Binding ◽  
Androgen Binding Protein ◽  
Androgen Binding
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