A comparison of morphology, pathogenicity and restriction fragment patterns of mitochondrial DNA among isolates of Phytophthora porri Foister

1992 ◽  
Vol 98 (5) ◽  
pp. 277-289 ◽  
Author(s):  
Arthur W. A. M. Cock ◽  
Afra Neuvel ◽  
Günther Bahnweg ◽  
Johanna C. J. M. Cock ◽  
Hermann H. Prell
Keyword(s):  
Mitochondrial Dna ◽  
Restriction Fragment ◽  
Phytophthora Porri

Mitochondrial and nuclear DNA variation, genotype fingerprinting and genetic relationships in lentil (Lens culinaris Medik.)

1997 ◽  
Vol 77 (4) ◽  
pp. 515-521 ◽  
Author(s):  
Om P. Rajora ◽  
John D. Mahon
Keyword(s):  
Mitochondrial Dna ◽  
Restriction Fragment ◽  
Lens Culinaris ◽  
Nuclear Dna ◽  
Genetic Relationships ◽  
Restriction Enzymes ◽  
Dna Variation ◽  
Apocytochrome B ◽  
Nuclear Dna Variation ◽  
Mean Similarity

Mitochondrial DNA (mtDNA) and nuclear DNA (nuDNA) variations were examined in six cultivars of Lens culinaris ssp. culinaris and two (mtDNA) or one (nuDNA) accession(s) of L. culinaris ssp. orientalis. Total leaf DNA was digested with up to 15 restriction endonucleases, separated by agarose gel electrophoresis and trasferred to nylon membranes. To examine mtDNA variation, blots were probed with mtDNA coding for cytochrome c oxidase I (coxI) and ATPase 6 (atp6) of both wheat and maize as well as apocytochrome b (cob) and Orf25 (orf25) of wheat. Sixteen combinations of mtDNA probes and restriction enzymes revealed 34 fragments that discriminated between at least two lentil accessions. For nuDNA analysis, probes from cDNA and genomic DNA clones of lentil were used to probe the same blots, and identified 46 diagnostic fragments from 19 probe/enzyme combinations. Each lentil accession could be unequivocably distinguished from all others on the basis of both mitochondrial and nuclear DNA fragment patterns. The mitochondrial restriction fragment similarities ranged from 0.944 to 0.989, with a mean of 0.970 but nuclear restriction fragment similarities varied from 0.582 to 0.987, with a mean of 0.743. The apparent genetic relationships among accessions differed according to the source of DNA examined, although the commercial varieties Laird, Brewer and Redchief showed similarly high levels of mean similarity with both nuclear (0.982) and mitochondrial DNA (0.983). Key words: Lens culinaris Medik., genetic variation, mitochondrial, nuclear, DNA, lentil

Restriction fragment length polymorphisms demonstrate single origin of infection centers in Phellinus weirii

1994 ◽  
Vol 72 (4) ◽  
pp. 440-447 ◽  
Author(s):  
Hanhong Bae ◽  
Everett M. Hansen ◽  
Steven H. Strauss
Keyword(s):  
Mitochondrial Dna ◽  
Restriction Fragment ◽  
Ribosomal Dna ◽  
Fragment Length ◽  
Host Species ◽  
Root Rot ◽  
Biological Species ◽  
Douglas Fir ◽  
Dna Probes ◽  
Restriction Fragment Length

Restriction fragment length polymorphism (RFLP) markers were used to study genetic variation in the basidiomycete fungus Phellinus weirii (Murr.) Gilbertson, the cause of laminated root rot of conifers. In an initial study, three isolates each from the Douglas-fir type and the cedar-type biological species were surveyed with 12 restriction enzymes and 20 random, mitochondrial, and nuclear-ribosomal gene probes. The two biological species were distinct with most probe–enzyme combinations (91%). Variation within biological species was detected for the random and ribosomal DNA probes but not for the mitochondrial DNA probes. In a subsequent study 65 probe–enzyme combinations (13 × 5) that had detected variation within the Douglas-fir type biological species were used to analyze 27 isolates derived from six infection centers, two host species, and two geographic areas in western Oregon. Infection centers differed from one another in numerous probe–enzyme combinations but were nearly genetically uniform within. Isolates from the two host species, Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) and mountain hemlock (Tsuga mertensiana (Bong.) Carr.), showed few RFLP differences. Initiation of infection centers, and subsequent vegetative or basdiospore initiated immigration, appear to be rare events. Key words: ribosomal DNA, mitochondrial DNA, RFLP, root rot, conifer.

Mitochondrial DNA variation and genetic relationships of Populus species

Genome ◽  
1993 ◽  
Vol 36 (1) ◽  
pp. 87-93 ◽  
Author(s):  
John W. Barrett ◽  
Om P. Rajora ◽  
F. C. H Yeh ◽  
Bruce P. Dancik ◽  
Curtis Strobeck
Keyword(s):  
Mitochondrial Dna ◽  
Restriction Fragment ◽  
Genetic Relationships ◽  
Mitochondrial Genomes ◽  
Nucleotide Substitutions ◽  
Dna Variation ◽  
Eastern Cottonwood ◽  
Length Polymorphisms ◽  
Populus Species ◽  
Atpase 6

We examined variation in and around the region coding for the cytochrome c oxidase I (coxI) and ATPase 6 (atp6) genes in the mitochondrial genomes of four Populus species (P. nigra, P. deltoides, P. maximowiczii, and P. tremuloides) and the natural hybrid P. × canadensis (P. deltoides × P. nigra). Total cellular DNAs of these poplars were digested with 16 restriction endonucleases and probed with maize mtDNA-specific probes (CoxI and Atp6). The only variant observed for Atp6 was interspecific, with P. maximowiczii separated from the other species as revealed by EcoRI digestions. No intraspecific mtDNA variation was observed among individuals of P. nigra, P. maximowiczii, P. × canadensis, or P. tremuloides for the CoxI probe. However, two varieties of P. deltoides were distinct because of a single site change in the KpnI digestions, demonstrating that P. deltoides var. deltoides (eastern cottonwood) and var. occidentalis (plains cottonwood) have distinct mitochondrial genomes in the region of the coxI gene. Populus × canadensis shared the same restriction fragment patterns as its suspected maternal parent P. deltoides. Nucleotide substitutions per base in and around the coxI and atp6 genes among the Populus species and the hybrid ranged from 0.0017 to 0.0077. The interspecific estimates of nucleotide substitution per base suggested that P. tremuloides was furthest removed from P. deltoides and P. × canadensis and least diverged from P. nigra. Populus maximowiczii was placed between these two clusters.Key words: mitochondrial DNA, poplars, phylogenetics, variation, restriction fragment length polymorphisms.

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