The effect of group selective reagentsN-ethylmaleimide and dithiothreitol on histamine H1-receptor binding sites in the vascular smooth muscle membranes

1984 ◽  
Vol 14 (3-4) ◽  
pp. 561-565 ◽  
Author(s):  
Marija Čarman-Kržan
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Receptor Binding ◽  
Binding Sites ◽  
H1 Receptor ◽  
Histamine H1 Receptor

Histamine H1 receptor binding sites in mouse uterine horns

1993 ◽  
Vol 24 (1) ◽  
pp. 29-33 ◽  
Author(s):  
S. Rodrigo Gonzalez ◽  
S. Karin Reinicke ◽  
G.M. Isolde Rudolph
Keyword(s):  
Receptor Binding ◽  
Binding Sites ◽  
H1 Receptor ◽  
Histamine H1 Receptor

Comparison of vasopressin binding sites in human uterine and vascular smooth muscle cells

1999 ◽  
Vol 378 (1) ◽  
pp. 137-142 ◽  
Author(s):  
Atsuo Tahara ◽  
Junko Tsukada ◽  
Noe Ishii ◽  
Yuichi Tomura ◽  
Koh-ichi Wada ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Binding Sites ◽  
Muscle Cells

Evidence for the existence of F2-isoprostane receptors on rat vascular smooth muscle cells

1993 ◽  
Vol 264 (6) ◽  
pp. C1619-C1624 ◽  
Author(s):  
M. Fukunaga ◽  
N. Makita ◽  
L. J. Roberts ◽  
J. D. Morrow ◽  
K. Takahashi ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Binding Sites ◽  
Tissue Injury ◽  
Specific Binding ◽  
Muscle Cells ◽  
Free Oxygen Radicals ◽  
Txa2 Receptor

The isoprostanes are nonenzymatically generated prostanoids synthesized in vivo in humans and rats through reactions catalyzed by free oxygen radicals. 8-Epi-prostaglandin F2 alpha (8-epi-PGF2 alpha), an F2-isoprostane, is a potent smooth muscle constrictor. A thromboxane A2 (TxA2) receptor antagonist, SQ 29548, blocks renal vasoconstriction during 8-epi-PGF2 alpha administration in rats. With the use of cultured rat aortic smooth muscle cells, we found specific binding sites for [3H]SQ 29548 and for [125I]BOP, a TxA2 agonist. Both ligands were displaced from these binding sites by 8-epi-PGF2 alpha, although with significantly lesser potency than nonlabeled SQ 29548, I-BOP, or U-46619, a TxA2 agonist. In contrast, 8-epi-PGF2 alpha stimulated inositol 1,4,5-trisphosphate production and DNA synthesis in these cells with significantly greater potency than any TxA2 agonist, effects only partially inhibited by SQ 29548. In human TxA2 receptor cDNA-transfected cells, competition by 8-epi-PGF2 alpha for specific [3H]SQ 29548 binding was negligible. Thus 8-epi-PGF2 alpha probably exerts its biological actions in vascular smooth muscle through activation of receptor sites related to but distinct from TxA2 receptors. The existence of such binding sites suggests novel avenues for investigation into the biology of TxA2 and of free radical-mediated tissue injury.

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