Inhibitory effects of phenyltin compounds on stimulus-induced changes in cytosolic free calcium and plasma membrane potential of human neutrophils

1991 ◽  
Vol 65 (7) ◽  
pp. 562-569 ◽  
Author(s):  
Yoshikazu Miura ◽  
Hisao Matsui
Keyword(s):  
Plasma Membrane ◽  
Membrane Potential ◽  
Human Neutrophils ◽  
Free Calcium ◽  
Inhibitory Effects ◽  
Cytosolic Free Calcium ◽  
Plasma Membrane Potential ◽  
Induced Changes

scholarly journals Silica Induces Changes in Cytosolic Free Calcium, Cytosolic pH, and Plasma Membrane Potential in Bovine Alveolar Macrophages

1997 ◽  
Vol 15 (2) ◽  
pp. 61-72 ◽  
Author(s):  
Attila Tárnok ◽  
Thomas Schlüter ◽  
Ingeborg Berg ◽  
Günther Gercken
Keyword(s):  
Plasma Membrane ◽  
Membrane Potential ◽  
Alveolar Macrophages ◽  
Specific Inhibitor ◽  
Dust Particles ◽  
Calcium Signal ◽  
Free Calcium ◽  
Cytosolic Free Calcium ◽  
Extracellular Medium ◽  
Plasma Membrane Potential

The mineral‐dust induced activation of pulmonary phagocytes is thought to be involved in the induction of severe lung diseases. The activation of bovine alveolar macrophages (BAM) by silica was investigated by flow cytometry. Short‐term incubation (10 min) of BAM with silica gel and quartz dust particles induced increases in the cytosolic free calcium concentration ([Ca2+]i), decreases in intracellular pH (pHi), and increases in plasma membrane potential (PMP). The extent of these changes was concentration dependent, related to the type of dust and was due to Ca2+influx from the extracellular medium. An increase in [Ca2+]iwas inhibited, when extracellular Ca2+was removed. Furthermore the calcium signal was quenched by Mn2+and diminished by the calcium channel blocker verapamil. The protein kinase C specific inhibitor bisindolylmaleimide II (GF 109203 X) did not inhibit the silica‐induced [Ca2+]irise. In contrast, silica‐induced cytosolic acidification and depolarization were inhibited by GF 109203 X but not by removal of extracellular calcium. Addition of TiO2particles or heavy metal‐containing dusts had no effect on any of the three parameters. Our data suggest the existence of silica‐activated transmembrane ion exchange mechanisms in BAM, which might be involved in the specific cytotoxicity of silica by Ca2+‐dependent and independent pathways.

Proton channels, plasma membrane potential, and respiratory burst in human neutrophils

2009 ◽  
Vol 51 (5) ◽  
pp. 309-312 ◽  
Author(s):  
Nicolas Demaurex ◽  
Jacques Schrenzel ◽  
Marisa E. Jaconi ◽  
Daniel P. Lew ◽  
Karl-Heinz Krause
Keyword(s):  
Plasma Membrane ◽  
Membrane Potential ◽  
Respiratory Burst ◽  
Human Neutrophils ◽  
Plasma Membrane Potential ◽  
Proton Channels

scholarly journals TRPC6 regulates phenotypic switching of vascular smooth muscle cells through plasma membrane potential‐dependent coupling with PTEN

2019 ◽  
Vol 33 (9) ◽  
pp. 9785-9796 ◽  
Author(s):  
Takuro Numaga‐Tomita ◽  
Tsukasa Shimauchi ◽  
Sayaka Oda ◽  
Tomohiro Tanaka ◽  
Kazuhiro Nishiyama ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Plasma Membrane ◽  
Membrane Potential ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Phenotypic Switching ◽  
Plasma Membrane Potential

scholarly journals Spontaneous and chemoattractant-induced oscillations of cytosolic free calcium in single adherent human neutrophils.

1988 ◽  
Vol 263 (22) ◽  
pp. 10557-10560 ◽  
Author(s):  
M E Jaconi ◽  
R W Rivest ◽  
W Schlegel ◽  
C B Wollheim ◽  
D Pittet ◽  
...  
Keyword(s):  
Human Neutrophils ◽  
Free Calcium ◽  
Cytosolic Free Calcium
Sign in / Sign up

Export Citation Format

Share Document