Immunocytochemical reaction of a haemocyanin antibody in the midgut gland ofNautilus (Cephalopoda, Tetrabranchiata)

1996 ◽  
Vol 52 (6) ◽  
pp. 549-553 ◽  
Author(s):  
P. Ruth ◽  
W. Blum ◽  
J. Bille
1986 ◽  
Vol 113 (4) ◽  
pp. 570-575 ◽  
Author(s):  
Firyal S. Khan-Dawood

Abstract. Immunoreactive oxytocin is detectable in the corpora lutea of women and cynomolgus monkeys by radioimmunoassay. To localize the presence of oxytocin and neurophysin I in ovarian tissues of subhuman primates, three corpora lutea and ovarian stromal tissues and two Fallopian tubes obtained during the menstrual cycle of the baboon and decidua from two pregnant baboons were examined using highly specific antisera against either oxytocin or neurophysin I and preoxidase-antiperoxidase light microscopy immunohistochemistry. Oxytocin-like as well as neurophysin I-like immunoreactivities were found in some cells of all the corpora lutea only, but could not be demonstrated in ovarian stromal tissues, Fallopian tubes and decidua. Specificity of the immunocytochemical reaction was further confirmed by immunoabsorption of the antiserum with excess oxytocin or neurophysin, after which the immunoreactivities for both oxytocin and neurophysin in the luteal tissue were negative. Similar controls using normal rabbit serum gave no positive staining for either oxytocin or neurophysin. Counterstaining of the positive immunoreactivities for oxytocin and neurophysin I with Mayer's haematoxylin and eosin demonstrated clearly that the oxytocin and neurophysin I appeared as granular material mainly within the cytoplasm of the luteal cells. The localization of immunoreactive oxytocin and neurophysin I in the corpus luteum of the baboon demonstrates directly the presence of these two neurohypophysial peptides within primate luteal cells and suggests their local production.


Zoomorphology ◽  
1985 ◽  
Vol 105 (5) ◽  
pp. 317-325 ◽  
Author(s):  
Annette Becker ◽  
Werner Peters
Keyword(s):  

2020 ◽  
Vol 18 ◽  
pp. 100507
Author(s):  
Maria Angélica Silva ◽  
Ramires Eloise Queiroz Rafael ◽  
Thiago Barbosa Cahú ◽  
Janilson Felix Silva ◽  
Karina Ribeiro ◽  
...  

1988 ◽  
Vol 74 (6) ◽  
pp. 751-754 ◽  
Author(s):  
Vittoria Donofrio ◽  
Umberto Passeretti ◽  
Sergio Russo ◽  
Amedeo Boscaino ◽  
Gaetano De Rosa

A benign tumor of nerve sheath origin occurring in the dermis of a thumb is described. Histologically, these lesions contained nests and cords of large cells surrounded by thin bands of collagen fibers scattered in a myxoid background. The immunocytochemical reaction for S-100 protein was positive.


1986 ◽  
Vol 1 (1) ◽  
pp. 9-14 ◽  
Author(s):  
Francesca Pietribiasi ◽  
Patrizia Gugliotta ◽  
Silvia Racca ◽  
Francesco Di Carlo ◽  
Gianni Bussolati

Correlation of structural and functional data might lead to better identification of hormone-dependent tumors. Sixty breast cancer specimens, sent to the biochemistry laboratory for estrogen receptor (ER) analysis, were studied here by a combined morpho-functional approach. Histological examination of needle biopsies on frozen tissue blocks showed that 12 cases (10%) were free of tumor cells; these cases mostly proved ER negative. On the other 48 cases, an immunocytochemical reaction was performed on the biopsy sections with a monoclonal antibody directed against p 29, an estrogen receptor related antigen. The staining values for p 29 and the biochemical ER findings were significantly correlated. A combined histological, immunocytochemical study seems to offer advantages in the selection of patients for hormonal therapy.


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