A simple method of DNA extraction from whole tissues and blood using glass powder for detection of transgenic animals by PCR

1995 ◽  
Vol 4 (2) ◽  
pp. 149-150 ◽  
Author(s):  
Joe Attal ◽  
Marco Cajero-Juarez ◽  
Louis-Marie Houdebine
Keyword(s):  
Dna Extraction ◽  
Glass Powder ◽  
Transgenic Animals ◽  
Simple Method

scholarly journals A Rapid Bacteriophage DNA Extraction Method

2018 ◽  
Vol 1 (3) ◽  
pp. 27 ◽  
Author(s):  
Džiuginta Jakočiūnė ◽  
Arshnee Moodley
Keyword(s):  
Dna Extraction ◽  
Illumina Miseq ◽  
Extraction Methods ◽  
Resistant Bacteria ◽  
Simple Method ◽  
Antibiotic Resistant ◽  
Phage Dna ◽  
Specialized Equipment ◽  
Miseq Platform ◽  
Dna Extraction Methods

Bacteriophages (phages) are intensely investigated as non-antibiotic alternatives to circumvent antibiotic resistance development as well as last resort therapeutic options against antibiotic resistant bacteria. As part of gaining a better understanding of phages and to determine if phages harbor putative virulence factors, whole genome sequencing is used, for which good quality phage DNA is needed. Traditional phage DNA extraction methods are tedious and time consuming, requiring specialized equipment e.g., an ultra-centrifuge. Here, we describe a quick and simple method (under four hours) to extract DNA from double stranded DNA (dsDNA) phages at titers above 1.0 × 1010 plaque-forming units (PFU)/mL. This DNA was suitable for library preparation using the Nextera XT kit and sequencing on the Illumina MiSeq platform.

scholarly journals Comparison of DNA extraction methods for Aspergillus fumigatus using real-time PCR

2006 ◽  
Vol 55 (9) ◽  
pp. 1187-1191 ◽  
Author(s):  
Lisa J. Griffiths ◽  
Martin Anyim ◽  
Sarah R. Doffman ◽  
Mark Wilks ◽  
Michael R. Millar ◽  
...  
Keyword(s):  
Real Time ◽  
Dna Extraction ◽  
Real Time Pcr ◽  
Diagnostic Technique ◽  
Extraction Methods ◽  
Simple Method ◽  
Bead Beating ◽  
Fungal Dna ◽  
Dna Extraction Methods ◽  
High Level

Newer methods such as PCR are being investigated in order to improve the diagnosis of invasive aspergillosis. One of the major obstacles to using PCR to diagnose aspergillosis is a reliable, simple method for extraction of the fungal DNA. The presence of a complex, sturdy cell wall that is resistant to lysis impairs extraction of the DNA by conventional methods employed for bacteria. Numerous fungal DNA extraction protocols have been described in the literature. However, these methods are time-consuming, require a high level of skill and may not be suitable for use as a routine diagnostic technique. Here, a number of extraction methods were compared: a freeze–thaw method, a freeze–boil method, enzyme extraction and a bead-beating method using Mini-BeadBeater-8. The quality and quantity of the DNA extracted was compared using real-time PCR. It was found that the use of a bead-beating method followed by extraction with AL buffer (Qiagen) was the most successful extraction technique, giving the greatest yield of DNA, and was also the least time-consuming method assessed.

scholarly journals A Simple Method for DNA Extraction from Mature Date Palm Leaves: Impact of Sand Grinding and Composition of Lysis Buffer

2010 ◽  
Vol 11 (9) ◽  
pp. 3149-3157 ◽  
Author(s):  
Ibrahim A. Arif ◽  
Mohammad A. Bakir ◽  
Haseeb A. Khan ◽  
Anis Ahamed ◽  
Ahmad H. Al Farhan ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Date Palm ◽  
Simple Method ◽  
Lysis Buffer

A Simple Method of DNA Extraction and STR Typing from Urine Samples Using a Commercially Available DNA/RNA Extraction Kit

2003 ◽  
Vol 48 (1) ◽  
pp. 2002184 ◽  
Author(s):  
Toshihiro Yasuda ◽  
Reiko Iida ◽  
Haruo Takeshita ◽  
Misuzu Ueki ◽  
Tamiko Nakajima ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Rna Extraction ◽  
Urine Samples ◽  
Str Typing ◽  
Simple Method

A rapid and simple method for small-scale DNA extraction inAgavaceae and other tropical plants

2002 ◽  
Vol 20 (3) ◽  
pp. 299-299 ◽  
Author(s):  
Miguel Keb-Llanes ◽  
Gerardo González ◽  
Bartolomé Chi-Manzanero ◽  
Diógenes Infante
Keyword(s):  
Dna Extraction ◽  
Small Scale ◽  
Simple Method ◽  
Tropical Plants

scholarly journals Sampling Tissue for DNA Analysis of Trees: Trunk Cambium as an Alternative to Canopy Leaves

2005 ◽  
Vol 54 (1-6) ◽  
pp. 265-269 ◽  
Author(s):  
N. Colpaert ◽  
S. Cavers ◽  
E. Bandou ◽  
H. Caron ◽  
G. Gheysen ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Tree Species ◽  
Dna Analysis ◽  
Leaf Tissue ◽  
Ground Level ◽  
Tropical Tree ◽  
Aflp Analysis ◽  
Simple Method ◽  
Tropical Tree Species ◽  
High Concentrations

Abstract The number of studies of tropical tree species that use molecular tools is increasing, most of which collect leaf tissue for genomic DNA extraction. In tropical trees the canopy is not only frequently inaccessible, but also, once reached, the leaf tissue is often heavily defended against herbivory by high concentrations of anti-predation compounds, which may inhibit downstream applications, particularly PCR. Cambium tissue, accessed directly from the tree trunk at ground level, offers a readily accessible resource that is less hampered by the presence of defensive chemicals than leaf tissue. Here we describe a simple method for obtaining tissue from the cambial zone for DNA extraction and test the applicability of the method in a range of tropical tree species. The method was used successfully to extract DNA from 11 species in nine families. A subset of the DNA extracts was tested in more detail and proved to be highly suitable for AFLP analysis.

A simple method of DNA extraction from Mycobacterium tuberculosis

1995 ◽  
Vol 76 (6) ◽  
pp. 578-581 ◽  
Author(s):  
M. Jaber ◽  
A. Rattan ◽  
A. Verma ◽  
J. Tyagi ◽  
R. Kumar
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Dna Extraction ◽  
Simple Method

An efficient and simple method of DNA extraction from whole blood and cell lines to identify infectious agents

1991 ◽  
Vol 34 (1) ◽  
pp. 105-112 ◽  
Author(s):  
V.N. Loparev ◽  
M.A. Cartas ◽  
C.E. Monken ◽  
A. Velpandi ◽  
A. Srinivasan
Keyword(s):  
Dna Extraction ◽  
Cell Lines ◽  
Whole Blood ◽  
Infectious Agents ◽  
Simple Method
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