Prostaglandin H synthase dependent metabolism of diethylstilbestrol by ram seminal vesicle cell cultures

1991 ◽  
Vol 65 (4) ◽  
pp. 344-347 ◽  
Author(s):  
J. Foth ◽  
G. H. Degen
Keyword(s):  
Seminal Vesicle ◽  
Cell Cultures ◽  
Prostaglandin H Synthase ◽  
Prostaglandin H

Study on N-Demethylation of N,N-Dimethyl-4-aminoazobenzene and N-Nitrosamines by Prostaglandin H Synthase

1997 ◽  
Vol 62 (6) ◽  
pp. 971-980 ◽  
Author(s):  
Marie Stiborová ◽  
Eva Frei ◽  
Heinz H. Schmeiser
Keyword(s):  
Hydrogen Peroxide ◽  
Arachidonic Acid ◽  
Free Radicals ◽  
Seminal Vesicle ◽  
Oxidation Mechanism ◽  
Maximal Velocity ◽  
Prostaglandin H Synthase ◽  
Michaelis Constant ◽  
Prostaglandin H

The in vitro enzymatic metabolism of carcinogenic N,N-dimethyl-4-aminoazobenzene, N-nitroso-N-methylaniline and N-nitroso-N,N-dimethylamine was investigated using ram seminal vesicle microsomal prostaglandin H synthase. Both N-nitrosamines are not converted by the studied enzyme. Formaldehyde is produced by the prostaglandin H synthase catalyzed reaction from N,N-dimethyl-4-aminoazobenzene. Arachidonic acid and hydrogen peroxide serve as cofactors for the oxidation of N,N-dimethyl-4-aminoazobenzene. The apparent Michaelis constant and the maximal velocity values for N,N-dimethyl-4-aminoazobenzene as a substrate are 64 μmol/l and 51.2 nmol HCHO/min/mg protein, respectively. In addition to formaldehyde, N-methyl-4-aminoazobenzene and 4-aminoazobenzene, two unknown substances are the products of the N,N-dimethyl-4-aminoazobenzene oxidation. The oxidation of N,N-dimethyl-4-aminoazobenzene catalyzed by prostaglandin H synthase is inhibited by glutathione, ascorbate and NADH. The results suggest that prostaglandin H synthase metabolizes N,N-dimethyl-4-aminoazobenzene through a one-electron oxidation mechanism, giving rise to free radicals.

scholarly journals Immunochemical evidence for the involvement of prostaglandin H synthase in hydroperoxide-dependent oxidations by ram seminal vesicle microsomes.

1983 ◽  
Vol 258 (10) ◽  
pp. 6517-6523 ◽  
Author(s):  
W R Pagels ◽  
R J Sachs ◽  
L J Marnett ◽  
D L Dewitt ◽  
J S Day ◽  
...  
Keyword(s):  
Seminal Vesicle ◽  
Prostaglandin H Synthase ◽  
Prostaglandin H

scholarly journals Heme coordination of prostaglandin H synthase.

1993 ◽  
Vol 268 (12) ◽  
pp. 8554-8563
Author(s):  
A.L. Tsai ◽  
R.J. Kulmacz ◽  
J.S. Wang ◽  
Y. Wang ◽  
H.E. Van Wart ◽  
...  
Keyword(s):  
Prostaglandin H Synthase ◽  
Prostaglandin H

scholarly journals Spectral analysis of the protein-derived tyrosyl radicals from prostaglandin H synthase.

1992 ◽  
Vol 267 (33) ◽  
pp. 23583-23588 ◽  
Author(s):  
J.A. DeGray ◽  
G Lassmann ◽  
J.F. Curtis ◽  
T.A. Kennedy ◽  
L.J. Marnett ◽  
...  
Keyword(s):  
Spectral Analysis ◽  
Prostaglandin H Synthase ◽  
Tyrosyl Radicals ◽  
Prostaglandin H

In vitro inhibition of prostaglandin H synthase by compounds from the exocrine secretions of lace bugs

1989 ◽  
Vol 93 (2) ◽  
pp. 253-255 ◽  
Author(s):  
Russell A. Jurenka ◽  
John W. Neal ◽  
Ralph W. Howard ◽  
James E. Oliver ◽  
Gary J. Blomquist
Keyword(s):  
Prostaglandin H Synthase ◽  
Exocrine Secretions ◽  
Lace Bugs ◽  
In Vitro Inhibition ◽  
Prostaglandin H
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