Total serum copper and ceruloplasmin levels following administration of copper aspirinate to rats and guinea-pigs

1980 ◽  
Vol 10 (5) ◽  
pp. 465-470 ◽  
Author(s):  
D. H. Brown ◽  
J. Dunlop ◽  
W. E. Smith ◽  
J. Teape ◽  
A. J. Lewis
Keyword(s):  
Guinea Pigs ◽  
Serum Copper ◽  
Total Serum ◽  
Copper Aspirinate

PARALLEL STUDIES OF COMPLEMENT AND COAGULATION IV. EFFECT OF CARBON TETRACHLORIDE

1951 ◽  
Vol 29 (2) ◽  
pp. 48-58
Author(s):  
Christine E. Rice ◽  
Paul Boulanger ◽  
P. J. G. Plummer
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Serum Protein ◽  
Guinea Pigs ◽  
Fatty Degeneration ◽  
Blood Groups ◽  
Total Serum Protein ◽  
Total Serum ◽  
Coagulation Time ◽  
Complement Activity

To determine whether liver injury would result in a parallel decline in the complement titer and coagulative properties of the blood, groups of guinea pigs were given series of injections of the liver poison, carbon tetrachloride. Marked fatty degeneration of the liver, a decline in total serum protein and albumin, a decrease in complement activity, and a prolongation of coagulation time was observed in the treated animals. A general relationship was noted between the albumin-globulin ratio and the complement titer of the serum and between the complement titer and the coagulation time of the plasma.

Influence of Estrogens on Total Serum Copper and Caeruloplasmin.

1956 ◽  
Vol 92 (3) ◽  
pp. 465-466 ◽  
Author(s):  
E. M. Russ ◽  
J. Raymunt
Keyword(s):  
Serum Copper ◽  
Total Serum

SEROLOGICAL EFFECTS OF ETHIONINE IN THE RABBIT

1954 ◽  
Vol 32 (2) ◽  
pp. 126-130 ◽  
Author(s):  
Christine E. Rice ◽  
P. Boulanger ◽  
E. Annau
Keyword(s):  
Body Weight ◽  
Protein Synthesis ◽  
Serum Albumin ◽  
Nitrogen Content ◽  
Guinea Pigs ◽  
Total Serum Protein ◽  
Total Serum ◽  
Serum Complement ◽  
Nonprotein Nitrogen ◽  
Normal Limits

In relation to body weight, rabbits were found to tolerate a much lower dosage of ethionine than guinea pigs. Serum complement titers fell, however, even in rabbits receiving small daily doses of ethionine, an indication of a basal disturbance in the processes of protein synthesis. Total serum protein values remained within normal limits but globulin tended to increase in relation to albumin, whereas in many of the ethionine-injected guinea pigs previously studied, serum albumin had increased in relation to globulin. In rabbits given 250 mgm. daily doses of ethionine, a definite increase in the nonprotein nitrogen content of the serum was recorded.

SEROLOGICAL EFFECTS OF ETHIONINE IN THE RABBIT

1954 ◽  
Vol 32 (1) ◽  
pp. 126-130
Author(s):  
Christine E. Rice ◽  
P. Boulanger ◽  
E. Annau
Keyword(s):  
Body Weight ◽  
Protein Synthesis ◽  
Serum Albumin ◽  
Nitrogen Content ◽  
Guinea Pigs ◽  
Total Serum Protein ◽  
Total Serum ◽  
Serum Complement ◽  
Nonprotein Nitrogen ◽  
Normal Limits

In relation to body weight, rabbits were found to tolerate a much lower dosage of ethionine than guinea pigs. Serum complement titers fell, however, even in rabbits receiving small daily doses of ethionine, an indication of a basal disturbance in the processes of protein synthesis. Total serum protein values remained within normal limits but globulin tended to increase in relation to albumin, whereas in many of the ethionine-injected guinea pigs previously studied, serum albumin had increased in relation to globulin. In rabbits given 250 mgm. daily doses of ethionine, a definite increase in the nonprotein nitrogen content of the serum was recorded.

STUDIES OF IRON REQUIREMENTS IN INFANTS AND CHILDREN

PEDIATRICS ◽  
1954 ◽  
Vol 13 (2) ◽  
pp. 107-125
Author(s):  
PHILLIP STURGEON
Keyword(s):  
Serum Iron ◽  
Binding Capacity ◽  
Serum Copper ◽  
Total Serum ◽  
Normal Infant ◽  
Iron Binding ◽  
Essentially Normal ◽  
Free Erythrocyte Protoporphyrin ◽  
Erythrocyte Protoporphyrin ◽  
Iron Binding Capacity

On an average the newborn infant serum compared to the normal adult male is hyperferremic (S.I. 193 µg./100 cc.), hypocupremic (S.C.51µg./100 cc.), and his free erythrocyte protoporphyrin is increased about two-fold (E.P. 55 µg./100 cc.). His total serum iron-binding capacity appears to be 50% or more saturated. At 12 hours of age marked hypoferremia (S.I. 46 µg./100 cc.) is present and the total iron-binding capacity as measured in this study is 100% saturated. There is slight, if any, change in the serum copper or erythrocyte protoporphyrin during the first few hours or days of life. From two weeks through one month of age an essentially normal pattern is present except for the slightly elevated (two-fold) protoporphyrin. By 4 to 10 months of age, on an average, the normal infant has the pattern characteristic of infancy, namely, hypoferremia (S.I., 50 µ. g/100 cc.), hypercupremia (S.C., 146 µg./100 cc.), reduced saturation of the total serum iron-binding capacity (12%) and elevated free erythrocyte protoporphyrin (E.P., 70 µg./100 cc.). Extreme ranges in values are found in different individuals at most ages. The suggested explanation for these findings is that on an average, the normal infant relative to the adult is totally depleted of his iron reserve and that his state of "physiologic anemia" constitutes an additional, but lesser, iron deficit.

Measurements of serum non-ceruloplasmin copper by a direct fluorescent method specific to Cu(II)

2017 ◽  
Vol 55 (9) ◽  
Author(s):  
Rosanna Squitti ◽  
Mariacristina Siotto ◽  
Emanuele Cassetta ◽  
Imane Ghafir El Idrissi ◽  
Nicola A. Colabufo
Keyword(s):  
Direct Method ◽  
Reference Interval ◽  
Serum Copper ◽  
Total Serum ◽  
Healthy Controls ◽  
Test Results ◽  
Fluorescent Method ◽  
Meta Analyses ◽  
The Brain ◽  
Test Result

AbstractBackground:Meta-analyses indicated the breakdown of copper homeostasis in the sporadic form of Alzheimer’s disease (AD), comprising copper decreases within the brain and copper increases in the blood and the pool not bound to ceruloplasmin (non-Cp Cu, also known in the literature as “free” copper). The calculated non-Cp Cu (Walshe’s) index has many limitations.Methods:A direct fluorescent method for non-Cp Cu detection has been developed and data are presented herein. The study included samples from 147 healthy subjects, 36 stable mild cognitive impairment (MCI) and 89 AD patients, who were tested for non-Cp Cu through the direct method, total serum copper, ceruloplasmin concentration andResults:The direct method was linear (0.9–5.9 μM), precise (within-laboratory coefficient variation of 9.7% for low and 7.1% for high measurements), and had a good recovery. A reference interval (0–1.9 μM) was determined parametrically in 147 healthy controls (27–84 years old). The variation of non-Cp Cu was evaluated according to age and sex. Non-Cp Cu was 1.5 times higher in AD patients (regarding the upper value of the reference interval) than in healthy controls. Healthy, MCI and AD subjects were differentiated through the direct non-Cp Cu method [areas under the curve (AUC)=0.755]. Considering a 95% specificity and a 1.91 μmol/L cut-off, the sensitivity was 48.3% (confidence interval 95%: 38%–58%). The likelihood ratio (LR) was 9.94 for positive test results (LR+) and 0.54 for negative test result (LR−).Conclusions:The direct fluorescent test reliably and accurately measures non-Cp Cu, thereby determining the probability of having AD.

scholarly journals Wilson's disease: the importance of measuring serum caeruloplasmin non-immunologically

2003 ◽  
Vol 40 (2) ◽  
pp. 115-121 ◽  
Author(s):  
J. M. Walshe
Keyword(s):  
Wilson’S Disease ◽  
Liver Function Tests ◽  
Wilson's Disease ◽  
Serum Copper ◽  
Full Blood Count ◽  
Total Serum ◽  
Clotting Factors ◽  
Urinary Copper ◽  
Specialist Centre ◽  
Close Relatives

Wilson's disease should be considered as a possible diagnosis in any child, adolescent or young adult with liver damage without other explanation, especially when haemolysis is present. However, it may also present in adolescents or young adults with neurological signs confined to the motor system. The first diagnostic screening test is the estimation of the serum caeruloplasmin and total serum copper concentrations, with calculation of the serum non-caeruloplasmin-bound ('free') copper. Serum caeruloplasmin, which contains copper, is best determined by measurement of its oxidase activity, as the immunonephelometric method measures both caeruloplasmin and the biologically inactive apo-form. Diagnosis may be confirmed by an elevated urinary copper excretion. All close relatives of an identified patient must be screened and, where doubt persists, investigation of the Wilson's gene at chromosome 13q14.3 can be employed. Lifelong follow-up studies are best conducted in a specialist centre. Compliance with chelating therapy (penicillamine or trientine) or administration of the metal antagonist tetrathiomolybdate or zinc is monitored by determination of the serum 'free' copper, which should be maintained at or near 1·6 µmol/L (10 µg/100 mL). Side-effects of therapy are detected by the estimation of urinary total protein, full blood count and erythrocyte sedimentation rate, clotting factors and liver function tests.

scholarly journals Possible Mechanism of Action of the Antiallergic Effect of an Aqueous Extract of Heliotropium indicum L. in Ovalbumin-Induced Allergic Conjunctivitis

2015 ◽  
Vol 2015 ◽  
pp. 1-7
Author(s):  
Samuel Kyei ◽  
George Asumeng Koffuor ◽  
Paul Ramkissoon ◽  
Samuel Abokyi ◽  
Osei Owusu-Afriyie ◽  
...  
Keyword(s):  
Guinea Pigs ◽  
Allergic Conjunctivitis ◽  
Clinical Score ◽  
Total Serum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Inflammatory Infiltration ◽  
Whole Plant ◽  
Antiallergic Effect ◽  
Heliotropium Indicum ◽  
Specific Igg

Heliotropium indicum is used traditionally as a remedy for conjunctivitis in Ghana. This study therefore evaluated the antiallergic potential of an aqueous whole plant extract of Heliotropium indicum (HIE) in ovalbumin-induced allergic conjunctivitis and attempted to predict its mode of action. Clinical scores for allergic conjunctivitis induced by intraperitoneal ovalbumin sensitization (100 : 10 μg OVA/Al(OH)3 in phosphate-buffered saline [PBS]) and topical conjunctival challenge (1.5 mg OVA in 10 μL PBS) in Dunkin-Hartley guinea pigs were estimated after a week’s daily treatment with 30–300 mg kg−1 HIE, 30 mg kg−1 prednisolone, 10 mg kg−1 chlorpheniramine, or 10 mL kg−1 PBS. Ovalbumin-specific IgG and IgE and total IgE in serum were estimated using Enzyme-Linked Immunosorbent Assay. Histopathological assessment of the exenterated conjunctivae was also performed. The 30 and 300 mg kg−1 HIE treatment resulted in a significantly (p≤0.001) low clinical score of allergic conjunctivitis. Ovalbumin-specific IgG and IgE as well as total serum IgE also decreased significantly (p≤0.01–0.001). The conjunctival tissue in HIE treated guinea pigs had mild mononuclear infiltration compared to the PBS-treated ones, which had intense conjunctival tissue inflammatory infiltration. HIE exhibited antiallergic effect possibly by immunomodulation or immunosuppression.

Influence of Sex Hormones on Total Serum Copper.

1959 ◽  
Vol 102 (1) ◽  
pp. 98-99 ◽  
Author(s):  
N. O. Johnson ◽  
T. Kheim ◽  
W. B. Kountz
Keyword(s):  
Sex Hormones ◽  
Serum Copper ◽  
Total Serum

scholarly journals Serum Copper and Serum Zinc in Preeclampsia: Cause or Effect?

2020 ◽  
pp. 52-57
Author(s):  
Anjum A. K. Sayyed ◽  
Alka N. Sontakke
Keyword(s):  
Trace Elements ◽  
Case Control Study ◽  
Serum Zinc ◽  
Serum Copper ◽  
Total Serum ◽  
Control Group ◽  
Multisystem Disorder ◽  
Mean Values ◽  
Copper And Zinc ◽  
Control Study

Background: Preeclampsia is multisystem disorder. Despite its prevalence and severity, the pathophysiology of this multisystem disorder is poorly understood. In concern regarding the increasing number of preeclamptic cases and lack of data about the levels of trace elements in preeclampsia, a case-control study was conducted with aim to determine the trace elements like serum total copper and serum total zinc in preeclampsia. Aims: To estimate alterations in serum copper and serum zinc in preeclampsia and to compare them with normal pregnant women. Study Design: This is a case control study, carried out in the Department of Biochemistry, MIMER Medical College, Talegaon Dabhade, Pune. Methods: The present study consisted of 120 study participants. These were divided into two groups. Group I - normal pregnant women as control (n=60) and Group II - preeclamptic group (n=60). The serum levels of copper and zinc were determined by inductively coupled plasma atomic emission spectrometry (ICP- AES) technique at IIT Mumbai. Results: Analysis revealed that mean values of total serum copper and total serum zinc were 196.20 ± 25.9 and 77.15 ± 14.5 (µg/dl) respectively in control group. In preeclamptic group, the mean values of copper and zinc were 213.13± 38.6 and 76.23 ± 13.13 (µg/dl) respectively. Copper was significantly increased in preeclamptic group, while non-significant reduction in levels of zinc levels was observed when compared to control group. Conclusion: In the present study, significantly high serum copper was observed in preeclamptic patients. Presence of high copper levels may be related factor in the etiopathogenesis of preeclampsia. Estimation of trace elements like copper and zinc may help clinicians in early diagnosis and minimizing or delaying complications of preeclampsia, hence preventing harm to both mother & fetus.

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