Typical and atypical mast cells of the rat gastrointestinal system: Distribution and correlation with tissue histamine

1984 ◽  
Vol 14 (1) ◽  
pp. 1-7 ◽  
Author(s):  
A. M. P. Saavedra-Delgado ◽  
S. Turpin ◽  
D. D. Metcalfe
Keyword(s):  
Mast Cells ◽  
Gastrointestinal System ◽  
Tissue Histamine

Distribution of mast cells in human heart auricles and correlation with tissue histamine

1988 ◽  
Vol 23 (3-4) ◽  
pp. 273-275 ◽  
Author(s):  
K. Rydzyński ◽  
B. Kolago ◽  
J. Zasŀonka ◽  
W. Kuroczyński
Keyword(s):  
Mast Cells ◽  
Human Heart ◽  
Tissue Histamine

Failure to show change in rat tissue histamine and serotonin after rapid decompression

1962 ◽  
Vol 203 (2) ◽  
pp. 389-390 ◽  
Author(s):  
Eric P. Kindwall ◽  
Lars Olof Boréus ◽  
Barbro Westerholm
Keyword(s):  
Mast Cells ◽  
Ambient Pressure ◽  
Control Group ◽  
Albino Rats ◽  
Morphological Appearance ◽  
High Ambient Pressure ◽  
Rapid Decompression ◽  
The Mean ◽  
Rat Tissue ◽  
Tissue Histamine

Albino rats were subjected to rapid decompression from high ambient pressure (6 atm). The mean levels of histamine and 5-hydroxytryptamine in skin, ileum, lung, and brain were measured. They showed no significant change as compared to a nondecompressed control group even when decompression time was short enough to cause immediate death. The decompression also failed to produce any changes in the morphological appearance of the mesenteric mast cells.

Ovarian, uterine and brain mast cells in female rats: Cyclic changes and contribution to tissue histamine

1998 ◽  
Vol 120 (2) ◽  
pp. 255-262 ◽  
Author(s):  
Yasemin Aydın ◽  
Neşe Tunçel ◽  
Firdevs Gürer ◽  
Muzaffer Tunçel ◽  
Müberra Koşar ◽  
...  
Keyword(s):  
Mast Cells ◽  
Female Rats ◽  
Cyclic Changes ◽  
Tissue Histamine

Cytochemical analysis of mast cell granules after poly-dl-lysine action

1978 ◽  
Vol 36 (2) ◽  
pp. 278-279
Author(s):  
R. Courtoy ◽  
L.J. Simar ◽  
J. Christophe
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Chemical Compounds ◽  
Cellular Membrane ◽  
Thin Sections ◽  
Organic Bases ◽  
Ferric Thiocyanate ◽  
Cytochemical Analysis ◽  
Mast Cell Granule ◽  
Amine Liberation

Several chemical compounds induce amine liberation from mast cells but do not necessarily provoque the granule expulsion. For example, poly-dl-lysine induces modifications of the cellular membrane permeability which promotes ion exchange at the level of mast cell granules. Few of them are expulsed but the majority remains in the cytoplasm and appears less dense to the electrons. A cytochemical analysis has been performed to determine the composition of these granules after the polylysine action.We have previously reported that it was possible to demonstrate polyanions on epon thin sections using a cetylpyridinium ferric thiocyanate method. Organic bases are selectively stained with cobalt thiocyanate and the sulfhydryle groups are characterized with a silver methenamine reaction. These techniques permit to reveal the mast cell granule constituents, i.e. heparin, biogenic amines and basic proteins.

The relationship of IGE receptor topography to signaling activity in RBL-2H3 mast cells

1991 ◽  
Vol 49 ◽  
pp. 236-237
Author(s):  
J.R. Pfeiffer ◽  
J.C. Seagrave ◽  
C. Wofsy ◽  
J.M. Oliver
Keyword(s):  
Mast Cells ◽  
Protein A ◽  
Scattered Electron ◽  
Ige Receptor ◽  
Receptor Complexes ◽  
Ige Binding ◽  
Electron Imaging ◽  
Small Clusters ◽  
Relationship Of ◽  
The Relationship

In RBL-2H3 rat leukemic mast cells, crosslinking IgE-receptor complexes with anti-IgE antibody leads to degranulation. Receptor crosslinking also stimulates the redistribution of receptors on the cell surface, a process that can be observed by labeling the anti-IgE with 15 nm protein A-gold particles as described in Stump et al. (1989), followed by back-scattered electron imaging (BEI) in the scanning electron microscope. We report that anti-IgE binding stimulates the redistribution of IgE-receptor complexes at 37“C from a dispersed topography (singlets and doublets; S/D) to distributions dominated sequentially by short chains, small clusters and large aggregates of crosslinked receptors. These patterns can be observed (Figure 1), quantified (Figure 2) and analyzed statistically. Cells incubated with 1 μg/ml anti-IgE, a concentration that stimulates maximum net secretion, redistribute receptors as far as chains and small clusters during a 15 min incubation period. At 3 and 10 μg/ml anti-IgE, net secretion is reduced and the majority of receptors redistribute rapidly into clusters and large aggregates.

Magnesium and sulfur in mast cell and basophil granules of lower vertebrates in relation with histamine

1992 ◽  
Vol 50 (2) ◽  
pp. 1596-1597
Author(s):  
Kenichi Takaya
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Mast Cell ◽  
Mast Cells ◽  
Tree Frog ◽  
X Ray ◽  
Transmission Electron ◽  
Scanning Transmission ◽  
Fresh Frozen ◽  
Ultrathin Sections

Mast cell and basophil granules of the vertebrate contain heparin or related sulfated proteoglycans. Histamine is also present in mammalian mast cells and basophils. However, no histamine is detected in mast cell granules of the amphibian or fish, while it is shown in those of reptiles and birds A quantitative x-ray microanalysis of mast cell granules of fresh frozen dried ultrathin sections of the tongue of Wistar rats and tree frogs disclosed high concentrations of sulfur in rat mast cell granules and those of sulfur and magnesium in the tree frog granules. Their concentrations in tree frog mast cell granules were closely correlated (r=0.94).Fresh frozen dried ultrathin sections and fresh air-dried prints of the tree frog tongue and spleen and young red-eared turtle (ca. 6 g) spleen and heart blood were examined by a quantitative energy-dispersive x-ray microanalysis (X-650, Kevex-7000) for the element constituents of the granules of mast cells and basophils. The specimens were observed by transmission electron microscopy (TEM) (80-200 kV) and followed by scanning transmission electron microscopy (STEM) under an analytical electron microscope (X-650) at an acceleration voltage of 40 kV and a specimen current of 0.2 nA. A spot analysis was performed in a STEM mode for 100 s at a specimen current of 2 nA on the mast cell and basophil granules and other areas of the cells. Histamine was examined by the o-phthalaldehyde method.

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