Direct demonstration of production of transforming growth factor activity by embryonic chick tissue

1988 ◽  
Vol 44 (4) ◽  
pp. 351-352 ◽  
Author(s):  
J. C. McLachlan ◽  
J. Macintyre ◽  
D. D. Hume ◽  
J. Smith
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor ◽  
Embryonic Chick ◽  
Factor Activity ◽  
Direct Demonstration ◽  
Chick Tissue

Determination of transforming growth factor activity in effusions from cancer patients

Cancer ◽  
1988 ◽  
Vol 61 (9) ◽  
pp. 1832-1837 ◽  
Author(s):  
Axel-R. Hanauske ◽  
Carlos L. Arteaga ◽  
Gary M. Clark ◽  
Joy Buchok ◽  
Martha Marshall ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cancer Patients ◽  
Transforming Growth Factor ◽  
Factor Activity

Promotion of embryonic chick limb cartilage differentiation by transforming growth factor-β

1989 ◽  
Vol 135 (2) ◽  
pp. 424-430 ◽  
Author(s):  
William M. Kulyk ◽  
Barbara J. Rodgers ◽  
Karen Greer ◽  
Robert A. Kosher
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β ◽  
Embryonic Chick ◽  
Cartilage Differentiation

Characterization of growth factor activity produced by fetal mouse osteoblasts

1990 ◽  
Vol 124 (2) ◽  
pp. 301-309 ◽  
Author(s):  
M. C. Slootweg ◽  
S. C. van Buul-Offers ◽  
C. M. Hoogerbrugge ◽  
M. P. M. Herrmann-Erlee ◽  
A. J. M. van den Eijnden-van Raaij ◽  
...  
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor ◽  
Primary Cultures ◽  
Bone Matrix ◽  
Transforming Growth Factor Β ◽  
Factor Activity ◽  
Fetal Mouse ◽  
Molecular Weights ◽  
3T3 Fibroblasts ◽  
Igf I

ABSTRACT Bone matrix contains a variety of growth factors, but little is known of osteoblastic production of such materials. The present study assesses growth factor activity, chromatographed on acidic Bio-Gel P-100, secreted into conditioned media of primary cultures of fetal mouse calvaria. The cultures produced insulin-like growth factor-I (IGF-I), determined by radioimmuno-assay of molecular weights 20 and 10 kDa. IGF-II, determined by radioreceptor assay, was present at 20–29 and 7 kDa. The IGF peaks at 20, 10 and 7 kDa were all mitogenic in MCF-7 cells. Proteins of several different molecular weights were also present that specifically bound IGF-I and IGF-II. Transforming growth factor-β (TGF-β), assayed in a system for inhibition of growth, was also produced. Both activated and latent forms were present, and part of the TGF-β was TGF-β2. The absence of mitogenic activity in the bmolecular range of platelet-derived growth factor, assayed in 3T3 fibroblasts, makes it unlikely that mouse osteoblasts produce this growth factor. Journal of Endocrinology (1990) 124, 301–309

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