Effect of protein kinase C activation and depletion on insulin stimulation of glycogen synthesis in cultured hepatoma cells

1988 ◽  
Vol 44 (1) ◽  
pp. 34-37 ◽  
Author(s):  
M. Caron ◽  
G. Cherqui ◽  
D. Wicek ◽  
J. Capeau ◽  
J. Bertrand ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Glycogen Synthesis ◽  
Hepatoma Cells ◽  
Insulin Stimulation ◽  
Kinase C ◽  
Protein Kinase C Activation ◽  
Stimulation Of

scholarly journals Involvement of Protein Kinase C Activation in L-Leucine-Induced Stimulation of Protein Synthesis in L6 Myotubes

2003 ◽  
Vol 43 (1-3) ◽  
pp. 97-103 ◽  
Author(s):  
Kazumi Yagasaki ◽  
Naoko Morisaki ◽  
Yoshiro Kitahara ◽  
Atsuhito Miura ◽  
Ryuhei Funabiki
Keyword(s):  
Protein Synthesis ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
L6 Myotubes ◽  
Kinase C ◽  
Protein Kinase C Activation ◽  
Stimulation Of

DAG accumulation from saturated fatty acids desensitizes insulin stimulation of glucose uptake in muscle cells

2001 ◽  
Vol 280 (2) ◽  
pp. E229-E237 ◽  
Author(s):  
Eulàlia Montell ◽  
Marco Turini ◽  
Mario Marotta ◽  
Matthew Roberts ◽  
Véronique Noé ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Fatty Acids ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Glucose Uptake ◽  
Insulin Response ◽  
Insulin Stimulation ◽  
Kinase C ◽  
Basal Glucose Uptake ◽  
Stimulation Of

The increased availability of saturated lipids has been correlated with development of insulin resistance, although the basis for this impairment is not defined. This work examined the interaction of saturated and unsaturated fatty acids (FA) with insulin stimulation of glucose uptake and its relation to the FA incorporation into different lipid pools in cultured human muscle. It is shown that basal or insulin-stimulated 2-deoxyglucose uptake was unaltered in cells preincubated with oleate, whereas basal glucose uptake was increased and insulin response was impaired in palmitate- and stearate-loaded cells. Analysis of the incorporation of FA into different lipid pools showed that palmitate, stearate, and oleate were similarly incorporated into phospholipids (PL) and did not modify the FA profile. In contrast, differences were observed in the total incorporation of FA into triacylglycerides (TAG): unsaturated FA were readily diverted toward TAG, whereas saturated FA could accumulate as diacylglycerol (DAG). Treatment with palmitate increased the activity of membrane-associated protein kinase C, whereas oleate had no effect. Mixture of palmitate with oleate diverted the saturated FA toward TAG and abolished its effect on glucose uptake. In conclusion, our data indicate that saturated FA-promoted changes in basal glucose uptake and insulin response were not correlated to a modification of the FA profile in PL or TAG accumulation. In contrast, these changes were related to saturated FA being accumulated as DAG and activating protein kinase C. Therefore, our results suggest that accumulation of DAG may be a molecular link between an increased availability of saturated FA and the induction of insulin resistance.

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