Persistence of added retinoids in organ culture media during induction of mucous metaplasia and glandular morphogenesis in hamster cheek pouches

1990 ◽  
Vol 46 (5) ◽  
pp. 513-517 ◽  
Author(s):  
M. E. Robinson ◽  
A. M. Verrinder ◽  
Gibbins ◽  
M. H. Hardy
Keyword(s):  
Organ Culture ◽  
Culture Media ◽  
Mucous Metaplasia ◽  
Cheek Pouches ◽  
Glandular Morphogenesis

Production of collagenase and inhibitor (TIMP) by intracranial tumors and dura in vitro

1983 ◽  
Vol 59 (3) ◽  
pp. 461-466 ◽  
Author(s):  
Ahmed N. Halaka ◽  
Rowena A.D. Bunning ◽  
Colin C. Bird ◽  
Myles Gibson ◽  
John J. Reynolds
Keyword(s):  
Organ Culture ◽  
Tumor Invasion ◽  
Culture Media ◽  
Intracranial Tumors ◽  
Short Term ◽  
Content Type ◽  
Collagenase Inhibitor ◽  
Tissue Degradation ◽  
Fine Print

✓ The production of collagenase and collagenase inhibitor (TIMP) by various intracranial tumors (25 meningiomas, eight gliomas, seven metastases, four pituitary adenomas, and five others) was studied in short-term organ culture. While meningiomas produced negligible amounts of collagenase, two metastatic carcinomas of bronchial and breast origin produced significant amounts of the enzyme. Cultures of dura from an invasive meningioma and of bone invaded by a meningioma also produced collagenase. In varying amounts, TIMP was detected in culture media from most of the tumors studied; invasive tumors tended to produce less TIMP than noninvasive tumors. The results are discussed in relation to current views on tissue degradation and mechanisms of tumor invasion.

scholarly journals Factors influencing the contamination rates of the conjunctival swabs and organ culture media of human donor eyes

Medicine ◽  
2018 ◽  
Vol 97 (38) ◽  
pp. e11879 ◽  
Author(s):  
Tobias Röck ◽  
Johanna Landenberger ◽  
Michael Buhl ◽  
Efdal Yoeruek ◽  
Karl Ulrich Bartz-Schmidt ◽  
...  
Keyword(s):  
Organ Culture ◽  
Culture Media ◽  
Human Donor ◽  
Factors Influencing

Bacterial Lipopolysaccharides in Sterile Corneal Organ-Culture Media

Cornea ◽  
1999 ◽  
Vol 18 (1) ◽  
pp. 92-114 ◽  
Author(s):  
Angela C. Sobottka Ventura ◽  
Matthias Böhnke
Keyword(s):  
Organ Culture ◽  
Culture Media ◽  
Corneal Organ Culture ◽  
Bacterial Lipopolysaccharides

Ultrastructure of Fetal Mouse Heart in Organ Culture: Effects of Anoxia

1974 ◽  
Vol 32 ◽  
pp. 30-31
Author(s):  
H.D. Sybers ◽  
J. Ingwall ◽  
M. DeLuca ◽  
J. Ross
Keyword(s):  
Organ Culture ◽  
Culture Media ◽  
Cardiac Metabolism ◽  
Mouse Heart ◽  
Suitable Model ◽  
Experimental Conditions ◽  
Fetal Mouse ◽  
Ultrastructural Studies ◽  
Culture Effects

Fetal mouse hearts (FMH) can be maintained in organ culture for several weeks, and may offer a suitable model for studying cardiac metabolism under experimental conditions. Ultrastructural studies were performed to establish the integrity of the cells which have been deprived of circulating blood and to examine the effects of anoxia. Mouse hearts from fetuses (18-20 day gestation) were maintained for 2 days in oxygenated culture media 199 containing insulin 50 ug/ml.

Proteins and enzymes of the brush-border membrane of mouse intestine: influence of organ culture on gel electrophoretic patterns

1982 ◽  
Vol 60 (4) ◽  
pp. 434-443 ◽  
Author(s):  
A. Berteloot ◽  
J. S. Hugon
Keyword(s):  
Membrane Proteins ◽  
Organ Culture ◽  
Intestinal Mucosa ◽  
Brush Border ◽  
Brush Border Membrane ◽  
Specific Activity ◽  
Culture Media ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Specific Marker

Purifications of mouse intestinal brush-border membranes from control explants and scrapings of intestinal mucosa have been compared. Based on the specific activity of sucrase used as a specific marker of these membranes, higher purification factors were obtained with control explants (24.7 ± 0.9) as compared with scrapings of intestinal mucosa (14.8 ± 0.9). However, similar patterns of proteins and enzymes were obtained by sodium dodecyl sulfate (SDS) – polyacrylamide gel electrophoresis after membrane solubilization by 2% SDS at room temperature. After 24 h of culture, higher molecular weight species of maltase–glucoamylase–isomaltase (band 4), alkaline phosphatase (bands 9–10), and trehalase (band 17) have been observed. Enzyme species appearing in the particulate fraction of culture media were, however, identical with those found at the brush-border membrane level in control explants, except for trehalase. These results are interpreted by considering the possible adsorption of serum components to brush-border membrane proteins. It thus appears that the membrane proteins and enzymes released in the media during organ culture are identical with those synthesized in the tissue in vitro or in vivo.

Contamination by Nonfermentative Microorganisms in M-K and Organ Culture Media

Cornea ◽  
1983 ◽  
Vol 2 (4) ◽  
pp. 299???302
Author(s):  
Mark A. Carter ◽  
Richard G. Lembach ◽  
Richard H. Keates
Keyword(s):  
Organ Culture ◽  
Culture Media

Somatomedin production by rat liver in organ culture

1982 ◽  
Vol 99 (3) ◽  
pp. 422-430 ◽  
Author(s):  
Michel Binoux ◽  
Claudine Lassarre ◽  
Nathalie Hardouin
Keyword(s):  
Organ Culture ◽  
Rat Liver ◽  
Culture Media ◽  
Gel Filtration ◽  
Base Line ◽  
Rat Serum ◽  
Competitive Protein Binding Assay ◽  
Dose Dependent Decrease ◽  
Dose Dependent

Abstract. Radioligand assays have been used to study the production of insulin-like growth factor (IGF) and its carrier (IGFCP) by rat liver in organ culture. Bound IGF released into the culture medium was dissociated and separated from its carrier by gel filtration in 1 m acetic acid. IGF was measured by a competitive protein-binding assay using human IGF and the specific IGFCP produced by rat liver. IGF CP concentrations were assessed in terms of IGFCP binding to labelled IGF, compared to a reference IGFCP preparation obtained from rat serum. Without added hormone,the ratios IGF:total protein and IGFCP:total protein in the culture media after 3 days' culture were approximately 7 and 70 times higher, respectively, than those in serum. GH and insulin both stimulated IGF production. The response was dose-dependent, and significant at physiological concentrations of hormone (10 ng/ml GH and 10 μU/ml insulin. With 1 μg/ml GH and 1 mU/ml insulin, the IGF concentrations in the media on average reached 2½ times the base-line level. These hormones had no significant effect on IGF CP production. One ng/ml cortisol stimulated IGF production, but, in response to increasing concentrations, there was a dose-dependent decrease in IGF production. By contrast, IGFCP production was stimulated and there was a positive correlation between the carrier concentration in the culture media and the amount of cortisol added. The results indicate that insulin and cortisol, in addition to GH, have a direct influence on IGF production by the liver in vitro. They also suggest that the biosyntheses of IGF and its carrier are subject to different systems of regulation.

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