Determination of 9-(1,3-dihydroxy-2-propoxymethyl)guanine (DHPG) in biological fluids by reversed-phase high pressure liquid chromatography

1987 ◽  
Vol 9 (5) ◽  
pp. 261-264 ◽  
Author(s):  
E. H. H. Wiltink ◽  
P. Stekkinger ◽  
J. A. C. Brakenhoff ◽  
S. A. Danner
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Biological Fluids ◽  
Reversed Phase

scholarly journals An accurate and sensitive analysis by high-pressure liquid chromatography of conjugated and unconjugated bilirubin IX-α in various biological fluids

1980 ◽  
Vol 185 (1) ◽  
pp. 281-284 ◽  
Author(s):  
S Onishi ◽  
S Itoh ◽  
N Kawade ◽  
K Isobe ◽  
S Sugiyama
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Glucuronic Acid ◽  
Biological Fluids ◽  
Reversed Phase ◽  
Complete Separation ◽  
Bile Pigments ◽  
Human Bile ◽  
Neonatal Liver

An accurate and sensitive method was developed for the complete separation of the native tetrapyrroles, such as bilirubin and its mono- and di-conjugates of glucuronic acid, glucose and xylose, by ion-pair reversed-phase high-pressure liquid chromatography. The application of this method was demonstrated by the analysis of bile pigments in human bile and urine, and the method also makes it possible to estimate very low UDP-glucuronyltransferase activity, such as is found in the human foetal and neonatal liver.

Determination of acetaminophen concentrations in serum by high-pressure liquid chromatography.

1977 ◽  
Vol 23 (9) ◽  
pp. 1596-1598 ◽  
Author(s):  
R A Horvitz ◽  
P I Jatlow
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Diethyl Ether ◽  
High Pressure Liquid Chromatography ◽  
Internal Standard ◽  
Reversed Phase ◽  
Single Extraction ◽  
Toxic Concentrations

Abstract We describe a method for determination of serum acetaminophen concentrations in serum by reversed phase high-pressure liquid chromatography. The homolog N-propionyl-p-aminophenol was used as an internal standard. The procedure, which requires only a single extraction with diethyl ether, can be optimized to be linear over the ranges of 10 to 100 or 1 to 20 mg/liter. Within-run CV was 1.2%; between-run CV was 4.4% and 4.9% at two different concentrations. Many commonly used drugs were tested and found not to interfere. The procedure is simple and rapid enough for use on an emergency basis in cases of overdosage, and can be optimized for measurement of either therapeutic or toxic concentrations.

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