Fusion polypeptides in gene cloning: Potential problems due to conformational alterations at the junction

1987 ◽  
Vol 43 (8) ◽  
pp. 920-922
Author(s):  
E. Querol ◽  
A. Parrilla
Keyword(s):  
Gene Cloning ◽  
Potential Problems ◽  
Fusion Polypeptides

Microbulldozing and Microchiseling

1969 ◽  
Vol 27 ◽  
pp. 134-135
Author(s):  
J.N. Ramsey ◽  
D.P. Cameron ◽  
F.W. Schneider
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Material Handling ◽  
Microprobe Analysis ◽  
Foreign Matter ◽  
Specific Location ◽  
Potential Problems ◽  
Knoop Indenter ◽  
Scanning Electron ◽  
Microhardness Tester

As computer components become smaller the analytical methods used to examine them and the material handling techniques must become more sensitive, and more sophisticated. We have used microbulldozing and microchiseling in conjunction with scanning electron microscopy, replica electron microscopy, and microprobe analysis for studying actual and potential problems with developmental and pilot line devices. Foreign matter, corrosion, etc, in specific locations are mechanically loosened from their substrates and removed by “extraction replication,” and examined in the appropriate instrument. The mechanical loosening is done in a controlled manner by using a microhardness tester—we use the attachment designed for our Reichert metallograph. The working tool is a pyramid shaped diamond (a Knoop indenter) which can be pushed into the specimen with a controlled pressure and in a specific location.

The “Mixing” of Implant Systems

1991 ◽  
Vol 4 (02) ◽  
pp. 38-45 ◽  
Author(s):  
F. Baumgart
Keyword(s):  
Stainless Steel ◽  
Additional Risk ◽  
Bone Screw ◽  
Application Technique ◽  
Quality Controls ◽  
Implant Materials ◽  
Osteosynthesis Plate ◽  
Potential Problems ◽  
Manufacturing Procedure

SummaryThe so-called “mixing” of implants and instruments from different producers entertain certain risks.The use of standardized implant materials (e.g. stainless steel ISO 5832/1) from different producers is necessary but is not sufficient to justify the use of an osteosynthesis plate from one source and a bone screw from another.The design, dimensions, tolerances, manufacturing procedure, quality controls, and application technique of the instruments and implants also vary according to make. This can lead to damage, failure or fracture of the biomechanical system called “osteosynthesis” and hence the failure of the treatment undertaken. In the end, it is the patient who pays for these problems.Some examples also illustrate the potential problems for the staff and institutions involved.The use of a unique, consistent, well-tested, and approved set of implants and instruments is to be strongly recommended to avoid any additional risk.

Commons Research and Pastoralism in the Context of Variability

2020 ◽  
Vol 24 (2) ◽  
pp. 272-285
Author(s):  
Jill Philine Blau
Keyword(s):  
Ecological Systems ◽  
Potential Problems ◽  
Pastoral Management ◽  
The Commons

Research has shown that pastoralism and the management of the commons are connected (Bollig and Lesogorol 2016). In this article I discuss how the concept of variability, which emerged from discussions of dryland ecologies in the 1980s (Homewood 2008), can inform and enhance research on the commons and vice versa. Research on the commons can further elucidate the understanding of pastoralist practices. I conclude with reflections drawn from some empirical examples in the literature, the use of the socio-ecological systems (SES) framework, and discuss the benefits and potential problems when applied to heterogeneous and flexible pastoralist practices and to the pastoral management of the commons.

Gene Cloning and Sequence Analysis of γ-Gliadin Genes from Wheat Cultivar Shaan 253

2010 ◽  
Vol 36 (3) ◽  
pp. 526-532
Author(s):  
Ming-Xia WANG ◽  
Xiang GAO ◽  
Qi-Jiao CHEN ◽  
Jian DONG ◽  
Wan-Chun ZHAO ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Gene Cloning ◽  
Wheat Cultivar
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