Effect of nerve stimulation on rat skeletal muscle. A study of plasma membrane

1985 ◽  
Vol 41 (11) ◽  
pp. 1396-1398 ◽  
Author(s):  
A. Shah ◽  
F. Nagao ◽  
V. Sahgal ◽  
H. Singh
Keyword(s):  
Skeletal Muscle ◽  
Plasma Membrane ◽  
Nerve Stimulation ◽  
Rat Skeletal Muscle

Insulin increases the Na(+)-K(+)-ATPase alpha 2-subunit in the surface of rat skeletal muscle: morphological evidence

1993 ◽  
Vol 265 (6) ◽  
pp. C1716-C1722 ◽  
Author(s):  
A. Marette ◽  
J. Krischer ◽  
L. Lavoie ◽  
C. Ackerley ◽  
J. L. Carpentier ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Plasma Membrane ◽  
Cellular Localization ◽  
Protein A ◽  
Morphological Evidence ◽  
Mammalian Skeletal Muscle ◽  
Tissue Fixation ◽  
Insulin Stimulation ◽  
Rat Skeletal Muscle ◽  
Quantitative Evidence

The cellular localization of the alpha 2-subunit of the Na(+)-K(+)-ATPase was defined by immunoelectron microscopy, and the effect of insulin on the amount of alpha 2-immunoreactive subunits on the cell surface was quantitated. Two protocols were used for tissue fixation and immunolocalization. Protocol 1 was characterized by fixation with 2% paraformaldehyde, use of a monoclonal antibody, and detection with 3-nm-diameter gold-labeled Fab fragments or 10-nm gold-labeled immunoglobulin G. Protocol 2 was characterized by fixation with 4% paraformaldehyde plus 0.1% glutaraldehyde, use of a polyclonal antibody, and detection with 10-nm gold-labeled protein A. In control muscle, the alpha 2-subunit of the Na(+)-K(+)-ATPase was present at the plasma membrane and in intracellular tubular and vesicular structures located in subsarcolemmal and triadic regions. Acute insulin stimulation increased the number of immunolabeled alpha 2-subunits in the plasma membrane after both fixation protocols. The gain in the plasma membrane ranged from 1.5- to 3.7-fold and was significant at the level of P < 0.005. These results provide morphological quantitative evidence that the alpha 2-subunit of the Na(+)-K(+)-ATPase is present both at the plasma membrane and intracellularly in mammalian skeletal muscle and that insulin acutely increases its abundance in the muscle surface.

scholarly journals Acute exercise increases the number of plasma membrane glucose transporters in rat skeletal muscle

FEBS Letters ◽  
1988 ◽  
Vol 238 (2) ◽  
pp. 235-239 ◽  
Author(s):  
Michael F. Hirshman ◽  
Harriet Wallberg-Henriksson ◽  
Lawrence J. Wardzala ◽  
Elizabeth D. Horton ◽  
Edward S. Horton
Keyword(s):  
Skeletal Muscle ◽  
Plasma Membrane ◽  
Acute Exercise ◽  
Glucose Transporters ◽  
Rat Skeletal Muscle

Acute endurance exercise increases plasma membrane fatty acid transport proteins in rat and human skeletal muscle

2012 ◽  
Vol 302 (2) ◽  
pp. E183-E189 ◽  
Author(s):  
Nicolette S. Bradley ◽  
Laelie A. Snook ◽  
Swati S. Jain ◽  
George J. F. Heigenhauser ◽  
Arend Bonen ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Plasma Membrane ◽  
Fatty Acid ◽  
Human Skeletal Muscle ◽  
Transport Proteins ◽  
Fatty Acid Transport ◽  
Acid Transport ◽  
Rat Skeletal Muscle ◽  
Muscle Plasma Membrane ◽  
Fatty Acid Transport Proteins

Fatty acid transport proteins are present on the plasma membrane and are involved in the uptake of long-chain fatty acids into skeletal muscle. The present study determined whether acute endurance exercise increased the plasma membrane content of fatty acid transport proteins in rat and human skeletal muscle and whether the increase was accompanied by an increase in long-chain fatty acid transport in rat skeletal muscle. Sixteen subjects cycled for 120 min at ∼60 ± 2% V̇o2 peak. Two skeletal muscle biopsies were taken at rest and again following cycling. In a parallel study, eight Sprague-Dawley rats ran for 120 min at 20 m/min, whereas eight rats acted as nonrunning controls. Giant sarcolemmal vesicles were prepared, and protein content of FAT/CD36 and FABPpm was measured in human and rat vesicles and whole muscle homogenate. Palmitate uptake was measured in the rat vesicles. In human muscle, plasma membrane FAT/CD36 and FABPpm protein contents increased 75 and 20%, respectively, following 120 min of exercise. In rat muscle, plasma membrane FAT/CD36 and FABPpm increased 20 and 30%, respectively, and correlated with a 30% increase in palmitate transport following 120 min of running. These data suggest that the translocation of FAT/CD36 and FABPpm to the plasma membrane in rat skeletal muscle is related to the increase in fatty acid transport and oxidation that occurs with endurance running. This study is also the first to demonstrate that endurance cycling induces an increase in plasma membrane FAT/CD36 and FABPpm content in human skeletal muscle, which is predicted to increase fatty acid transport.

scholarly journals Characterization of L-carnitine transport into rat skeletal muscle plasma membrane vesicles

2000 ◽  
Vol 267 (7) ◽  
pp. 1985-1994 ◽  
Author(s):  
Simona Berardi ◽  
Bruno Stieger ◽  
Bruno Hagenbuch ◽  
Ernesto Carafoli ◽  
Stephan Krähenbühl
Keyword(s):  
Skeletal Muscle ◽  
Plasma Membrane ◽  
Membrane Vesicles ◽  
Rat Skeletal Muscle ◽  
Plasma Membrane Vesicles ◽  
Muscle Plasma Membrane ◽  
Carnitine Transport

Glucose transport into rat skeletal muscle: interaction between exercise and insulin

1988 ◽  
Vol 65 (2) ◽  
pp. 909-913 ◽  
Author(s):  
H. Wallberg-Henriksson ◽  
S. H. Constable ◽  
D. A. Young ◽  
J. O. Holloszy
Keyword(s):  
Skeletal Muscle ◽  
Plasma Membrane ◽  
Glucose Transport ◽  
Transport Process ◽  
Sugar Transport ◽  
Glucose Transporters ◽  
Mammalian Skeletal Muscle ◽  
Rat Skeletal Muscle ◽  
Wide Range ◽  
Exercise Induced

This study was done to evaluate the effect of insulin on sugar transport into skeletal muscle after exercise. The permeability of rat epitrochlearis muscle to 3-O-methylglucose (3-MG) was measured after exposure to a range of insulin concentrations 30, 60, and 180 min after a bout of exercise. Thirty and 60 min after exercise, the effects of exercise and insulin on 3-MG transport were additive over a wide range of insulin concentrations, with no increase in sensitivity or responsiveness to insulin. After 180 min, when approximately 66% of the exercise-induced increase in sugar transport had worn off, both the responsiveness and sensitivity of the glucose transport process to insulin were increased. These findings appear compatible with the hypothesis that the actions of exercise and insulin result in activation and/or translocation into the plasma membrane of two separate pools of glucose transporters in mammalian skeletal muscle.

scholarly journals Periapical lesions decrease Akt serine phosphorylation and plasma membrane GLUT4 content in rat skeletal muscle

2015 ◽  
Vol 20 (7) ◽  
pp. 1625-1630 ◽  
Author(s):  
Renato Felipe Pereira ◽  
Max Sander de Oliveira da Mota ◽  
Maria Sara de Lima Coutinho Mattera ◽  
Thaís Verônica Saori Tsosura ◽  
Fernando Yamamoto Chiba ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Plasma Membrane ◽  
Serine Phosphorylation ◽  
Rat Skeletal Muscle ◽  
Periapical Lesions
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