Vitamin E, catalase, manganous or cobaltous ions and dithiothreitol protect against Tween 20-induced hemolysis of vitamin E-deficient chick and kid erythrocytes

1984 ◽  
Vol 40 (3) ◽  
pp. 258-259 ◽  
Author(s):  
T. Hamada ◽  
K. Hodate ◽  
E. Nakayama
Keyword(s):  
Vitamin E ◽  
Tween 20

The Entrapment of Vitamin E in Nanostructured Lipid Carriers of Rambutan Seed Fat for Cosmeceutical Uses

2016 ◽  
Vol 675-676 ◽  
pp. 77-80 ◽  
Author(s):  
Kwansiri Uraiwan ◽  
Chutimon Satirapipathkul
Keyword(s):  
Vitamin E ◽  
Solid Lipid Nanoparticles ◽  
Particle Diameter ◽  
Lipid Nanoparticles ◽  
Nanostructured Lipid Carriers ◽  
Chemical Effect ◽  
Tween 20 ◽  
Solvent Ratio ◽  
Nephelium Lappaceum ◽  
Mean Particle Diameter

The aim of the present work was to extract the seed fat of Rambutan (Nephelium lappaceum L.) for developing nanostructured lipid carriers. Effect of material: solvent ratio and material size time on fat content were investigated. Nanostructured lipid carriers were prepared by melt-emulsification technique. Stearic acid (SA) and the rambutan seed fat (RF) was used as solid and liquid lipid, respectively. Surfactant was Tween 20 as well as Vitamin E was entrapment chemical. Effect of surfactant concentration on formation and stability of solid lipid nanoparticles (SLN) was investigated. At higher surfactant concentrations, NLC had smaller particle size and better stability of nanoparticles. At the optimal concentration of Tween 20 (5% w/w), NLC had a mean particle diameter of 139.43 ± 1.15 nm and a polydispersity index of 0.165 ± 0.017. Zeta potential was above 30 mV.

Continuous Encapsulation of Vitamin E Using Polycaprolactone and Tween 20 in a Micro-Channel

2022 ◽  
Author(s):  
Amaraporn Kaewchada ◽  
Rotsaman Chongcharoen ◽  
Preuk Tangpromphan ◽  
Khwanchanok Nakkong ◽  
Attasak Jaree
Keyword(s):  
Vitamin E ◽  
Aqueous Phase ◽  
Residence Time ◽  
Organic Phase ◽  
Biological Activities ◽  
Continuous Process ◽  
Operating Conditions ◽  
Tween 20 ◽  
Micro Channel ◽  
Factors Affecting

Encapsulation of vitamin E is the preservation of the biological activities of vitamin E for various applications. In the first part of this research, factors affecting the batch encapsulation of vitamin E, including PCL concentration, the concentration of Tween 20, and the volumetric ratio of aqueous phase to organic phase were experimentally investigated. The Box-Behnken experimental design and response surface methodology were implemented to determine the optimal operating conditions of the batch encapsulation. At the optimal conditions, the percentage of vitamin E encapsulation (%EC) was 98.69%, using the PCL concentration, the Tween 20 concentration, and the volumetric ratio of aqueous phase to organic phase of 3.6 g/L, 0.6 g/L, and 0.9 mL: 1 mL, respectively. The second part is to enhance the productivity by applying the optimized formulation of vitamin E encapsulation in a continuous process using a micro-channel encapsulator. The effect of residence time was investigated. At the residence time of 1 s, the percentage of vitamin E encapsulation of 97.28% and the productivity of 153.61 mg/(mL∙min) were achieved.

scholarly journals Effect of Plasma Vitamin E Levels on Tween 20 Hemolysis of Goat Erythrocytes

1985 ◽  
Vol 56 (6) ◽  
pp. 468-471
Author(s):  
Kyoko HODATE ◽  
Tetsuo HAMADA
Keyword(s):  
Vitamin E ◽  
Tween 20 ◽  
Plasma Vitamin ◽  
Plasma Vitamin E

scholarly journals Vitamin E-based Folic Acid Nanoemulsion: Formulation and Physical Evaluation for Oral Administration

2019 ◽  
Vol 7 (4) ◽  
pp. 304-313
Author(s):  
Annis Catur Adi ◽  
Christanto Christanto ◽  
Heni Rachmawati ◽  
Amirah Adlia
Keyword(s):  
Folic Acid ◽  
Vitamin E ◽  
Light Exposure ◽  
Entrapment Efficiency ◽  
Tween 20 ◽  
Acid Stability ◽  
Peg 400 ◽  
Globule Size ◽  
Optimum Formula ◽  
Physical And Chemical

Background: Folic acid is essential in many metabolic processes and DNA synthesis. Nevertheless, folic acid is not stable, pH-sensitive, and deteriorated upon light exposure. Objective: This work was aimed to improve folic acid stability within vitamin E-based nanoemulsion. Methods: The nanoemulsion was prepared with self-nanoemulsification method by mixing vitamin E oil, Tween 20, and PEG 400. A pseudoternary phase diagram was constructed with aqueous titration to determine the optimum ratio for the mixture. The globule size, pH and entrapment efficiency were included in the nanoemulsion characterizations. In addition, the influence of centrifugation, storage, and pH on physical and chemical stabilities of folic acid nanoemulsion was evaluated. Results: Optimum formula was obtained from vitamin E, Tween 20, and PEG 400 with the ratio of 1:11:1, and the folic acid amount was 8 mg. The size of folic acidloaded oil globule was 15.10 ± 1.51 nm, and the nanoemulsion pH was 6.24 ± 0.01. The nanoemulsion system was able to load the folic acid completely. Folic acid in nanoemulsion was stable after 14 days at room temperature, and it was more stable compared to folic acid in solution. In addition, the physical and chemical characteristics of folic acid in nanoemulsion was not affected by the simulated gastric condition. Conclusion: Hence, nanoemulsion is a promising strategy to enhance folic acid stability.

An immunocytochemical study of maternal immunoglobulin localization in the suckling pig intestine

1990 ◽  
Vol 48 (3) ◽  
pp. 922-923
Author(s):  
László G. Kömüves ◽  
Donna S. Turner ◽  
Kathy S. McKee ◽  
Buford L. Nichols ◽  
Julian P. Heath
Keyword(s):  
Sodium Ethoxide ◽  
Intracellular Localization ◽  
Protein A ◽  
Tween 20 ◽  
Intestinal Epithelial ◽  
Immunocytochemical Study ◽  
Fish Skin ◽  
Newborn Piglets ◽  
Rabbit Igg ◽  
Fish Skin Gelatin

In this study we used colloidal gold probes to detect the intracellular localization of colostral immunoglobulins in intestinal epithelial cells of newborn piglets.Tissues were obtained from non-suckled newborn and suckled piglets aged between 1 hour to 1 month. Samples were fixed in 2.5 % glutaraldehyde, osmicated and embedded into Spurr’s resin. Thin (80 nm) sections were etched with 5% sodium ethoxide for 5 min, washed and treated with 4 % sodium-m-periodate in distilled water for 30 min. The sections were then first incubated with blocking buffer (2 % BSA, 0.25 % fish skin gelatin, 0.5 % Tween 20 in 10 mM Trizma buffer, pH=7.4 containing 500 mM NaCl) for 30 min followed by the immunoreagents diluted in the same buffer, 1 hr each. For the detection of pig immunoglobulins a rabbit anti-pig IgG antiserum was used followed by goat anti-rabbit IgG-Au10 or protein A-Au15 probes.

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