The assessment of tobacco smoke toxicity in organ culture. II. Ultrastructural studies on the immediate response of foetal rabbit tracheal epithelium to short-term exposures of whole smoke

1975 ◽  
Vol 31 (6) ◽  
pp. 682-684 ◽  
Author(s):  
P. Davies ◽  
G. S. Kistler
Keyword(s):  
Organ Culture ◽  
Tobacco Smoke ◽  
Tracheal Epithelium ◽  
Smoke Toxicity ◽  
Short Term ◽  
Ultrastructural Studies ◽  
Whole Smoke ◽  
Immediate Response

PROLACTIN SECRETION AND SYNTHESIS IN SHORT- AND LONG-TERM ORGAN CULTURE OF PITUITARY TUMOURS FROM ACROMEGALIC PATIENTS

1978 ◽  
Vol 87 (4) ◽  
pp. 701-715 ◽  
Author(s):  
F. Peillon ◽  
F. Cesselin ◽  
P. E. Garnier ◽  
A. M. Brandi ◽  
M. Donnadieu ◽  
...  
Keyword(s):  
Organ Culture ◽  
Prolactin Secretion ◽  
Pituitary Tumours ◽  
Short Term ◽  
Ultrastructural Studies ◽  
Gh Cells ◽  
Short And Long Term

ABSTRACT To evaluate the in vitro PRL secretion and synthesis by pituitary tumours from acromegalic patients, six somatotrophic and two somatomammotrophic adenomas were collected after surgery and divided into fragments 1 mm3 in size. Fragments were cultured in short-term (1–2–3–4 h) and in long-term organ culture (7 and 14 days). Media were collected for GH and PRL radioimmunoassay and fragments studied by electron microscopy. In two experiments, fragments from a somatotrophic adenoma and from a somatomammotrophic adenoma were cultured for 1 to 4 h and 9 to 16 days with [3H]leucine. In addition the effect of somatostatin (2.5 nmole/ml) upon PRL secretion was studied in short-term incubation. In long-term organ culture PRL concentrations increased in 8 out of 16 media samples collected from the five tumours and one normal pituitary cultured for 14 days, while GH concentrations decreased during the same time in all the experiments. The [3H]PRL/[3H] proteins ratios were 9.7 % on the 9th day of culture and 20.8 % on the 16th day while for GH, the ratios were respectively 40 and 34.7 %. Ultrastructural studies showed, besides GH cells, the presence of PRL secreting cells in the different tumours, after 7 or 14 days of culture. In short-term incubation, PRL concentrations in media increased in most of the samples collected from the three tumours incubated for 1 to 4 h and the [3H]PRL/[3H]proteins ratio was 10.6 % after 1 h and 21 % after 2 h of incubation. A decrease of PRL content (< 50 %) was observed in two of the three tumours incubated with somatostatin. These data indicate that 1) somatotrophic adenomas, as well as somatomammotrophic ones, are able to secrete and synthesize PRL in vitro, 2) PRL synthesis from these adenomas increases during culture, as from normal pituitary, presumably because of the release of PRL-secreting cells from the PRL inhibitory hypothalamic control (PIF), 3) somatostatin may reduce PRL release in vitro from somatotrophic adenomas as reported in some cases in vivo.

IN VITRO METHODS FOR THE STUDY OF THE ADENOHYPOPHYSIAL FUNCTIONS TO SECRETE GONADOTROPHIN

1971 ◽  
Vol 68 (1_Suppl) ◽  
pp. S27-S40 ◽  
Author(s):  
T. Kobayashi ◽  
T. Kigawa ◽  
M. Mizuno ◽  
T. Watanabe
Keyword(s):  
Cell Culture ◽  
Organ Culture ◽  
Cultured Cells ◽  
Cell Sheet ◽  
Short Term ◽  
Hypothalamic Extract ◽  
Numerous Cell ◽  
Single Cell Culture ◽  
Almost All

ABSTRACT There are several in vitro methods to analyse the function of the adenohypophysis or the mechanisms of its regulation. The present paper deals with single cell culture, organ culture and short term incubation techniques by which the morphology and gonadotrophin-secreting function of the adenohypophysis were studied. In trypsin-dispersed cell culture, the adenohypophysial cells showed extensive propagation to form numerous cell colonies and finally develop into a confluent monolayer cell sheet covering completely the surface of culture vessels. Almost all of the cultured cells, however, became chromophobic, at least at the end of the first week of cultivation, when gonadotrophin was detectable neither in the culture medium nor in the cells themselves. After the addition of the hypothalamic extract, gonadotrophin became detectable again, and basophilic or PAS-positive granules also reappeared within the cells, suggesting that the gonadotrophs were stimulated by the extract to produce gonadotrophin. In organ culture and short term incubation, the incorporation of [3H] leucine into the adenohypophysial cells in relation to the addition of hypothalamic extract was examined. It was obvious that the ability to incorporate [3H] leucine into the gonadotrophs in vitro was highly dependent upon the presence of the hypothalamic extract.

Vitamin A and β-carotene influence the level of benzo[a]pyrene-induced DNA adducts and DNA-repair activities in hamster tracheal epithelium in organ culture

1995 ◽  
Vol 91 (2) ◽  
pp. 205-214 ◽  
Author(s):  
Andreas P.M. Wolterbeek ◽  
Robert Roggeband ◽  
Catherina J.A. van Moorsel ◽  
Robert A. Baan ◽  
Jan H. Koeman ◽  
...  
Keyword(s):  
Dna Repair ◽  
Vitamin A ◽  
Organ Culture ◽  
Dna Adducts ◽  
Tracheal Epithelium ◽  
Β Carotene

scholarly journals Development of a Short-Term Canine Full-Thickness Skin Organ Culture Method under Serum-Free Conditions

2016 ◽  
Vol 11 (2) ◽  
pp. 61-69 ◽  
Author(s):  
Francesca Abramo ◽  
Andrea Pirone ◽  
Carla Lenzi ◽  
Maria Federica della Valle ◽  
Silvia Vidali ◽  
...  
Keyword(s):  
Organ Culture ◽  
Culture Method ◽  
Full Thickness ◽  
Short Term ◽  
Serum Free ◽  
Full Thickness Skin ◽  
Thickness Skin ◽  
Serum Free Conditions ◽  
Skin Organ Culture

Organization of tracheal epithelium in the cartilaginous portion of adult rabbit and its persistence in organ culture

1994 ◽  
Vol 238 (4) ◽  
pp. 463-472 ◽  
Author(s):  
Russell A. Johnson ◽  
Ziva Stauber ◽  
S. Robert Hilfer ◽  
Steven G. Kelsen
Keyword(s):  
Organ Culture ◽  
Tracheal Epithelium ◽  
Adult Rabbit
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