Histochemical demonstration of adrenergic nerve fibres and serotonin-containing mast cells of the knee joint synovial membrane in rats

1977 ◽  
Vol 33 (1) ◽  
pp. 52-53 ◽  
Author(s):  
Seisuke Tanaka ◽  
Norio Ohnishi ◽  
Yoshinori Tominaga
Keyword(s):  
Mast Cells ◽  
Knee Joint ◽  
Synovial Membrane ◽  
Histochemical Demonstration ◽  
Adrenergic Nerve ◽  
Nerve Fibres

Adrenergic nerve fibres and mast cells:

2002 ◽  
Vol 84 (1) ◽  
pp. 69-76 ◽  
Author(s):  
Marco Artico ◽  
Carlo Cavallotti ◽  
Daniela Cavallotti
Keyword(s):  
Mast Cells ◽  
Adrenergic Nerve ◽  
Nerve Fibres

Effects of Osmium Tetroxide on the Rabbit Knee Joint Normal Synovial Membrane

1987 ◽  
Vol 16 (1) ◽  
pp. 121-129
Author(s):  
M. Möttönen ◽  
M. Pantio ◽  
T. Nevalainen
Keyword(s):  
Knee Joint ◽  
Synovial Membrane ◽  
Osmium Tetroxide ◽  
Rabbit Knee

scholarly journals The role of oxidative and nitrosative stress in the pathology of osteoarthritis: Novel candidate biomarkers for quantification of degenerative changes in the knee joint

2018 ◽  
Vol 10 (2) ◽  
Author(s):  
Alexander Franz ◽  
Laura Joseph ◽  
Constantin Mayer ◽  
Jan-Frieder Harmsen ◽  
Holger Schrumpf ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Synovial Fluid ◽  
Knee Joint ◽  
Human Serum ◽  
Synovial Membrane ◽  
Nitrosative Stress ◽  
Control Groups ◽  
Oxidative And Nitrosative Stress ◽  
And Oxidative Stress

Osteoarthritis (OA) is the most frequently diagnosed joint disorder worldwide with increasing prevalence and crucial impact on the quality of life of affected patients through chronic pain, decreasing mobility and invalidity. Although some risk factors, such as age, obesity and previous joint injury are well established, the exact pathogenesis of OA on a cellular and molecular level remains less understood. Today, the role of nitrosative and oxidative stress has not been investigated conclusively in the pathogenesis of OA yet. Therefore, the objective of this study was to identify biological substances for oxidative and nitrosative stress, which mirror the degenerative processes in an osteoarthritic joint. 69 patients suffering from a diagnosed knee pain participated in this study. Based on the orthopedic diagnosis, patients were classified into an osteoarthritis group (OAG, n=24) or in one of two control groups (meniscopathy, CG1, n=11; anterior cruciate ligament rupture, CG2, n=34). Independently from the study protocol, all patients underwent an invasive surgical intervention which was used to collect samples from the synovial membrane, synovial fluid and human serum. Synovial biopsies were analyzed histopathologically for synovitis (Krenn-Score) and immunohistochemically for detection of end products of oxidative (8-isoprostane F2α) and nitrosative (3-nitrotyrosine) stress. Additionally, the fluid samples were analyzed for 8-isoprostane F2α and 3-nitrotyrosine by competitive ELISA method. The analyzation of inflammation in synovial biopsies revealed a slight synovitis in all three investigated groups. Detectable concentrations of 3-nitrotyrosine were reported in all three investigated groups without showing any significant differences between the synovial biopsies, fluid or human serum. In contrast, significant increased concentrations of 8-isoprostane F2α were detected in OAG compared to both control groups. Furthermore, our data showed a significant correlation between the histopathological synovitis and oxidative stress in OAG (r=0.728, P<0.01). There were no significant differences between the concentrations of 8-isoprostane F2α in synovial fluid and human serum. The findings of the current study support the hypothesis that oxidative and nitrosative stress are components of the multi-factory pathophysiological formation of OA. It seems reasonable that an inflammatory process in the synovial membrane triggers the generation of oxidative and nitrosative acting substances which can lead to a further degradation of the articular cartilage. Based on correlations between the observed degree of inflammation and investigated biomarkers, especially 8-isoprostane F2α seems to be a novel candidate biomarker for OA. However, due to the finding that also both control groups showed increased concentrations of selected biomarkers, future studies have to validate the diagnostic potential of these biomarkers in OA and in related conditions of the knee joint.

scholarly journals Aged human skin accumulates mast cells with altered functionality that localize to macrophages and vasoactive intestinal peptide‐positive nerve fibres

2018 ◽  
Vol 180 (4) ◽  
pp. 849-858 ◽  
Author(s):  
S.M. Pilkington ◽  
M.J. Barron ◽  
R.E.B. Watson ◽  
C.E.M. Griffiths ◽  
S. Bulfone‐Paus
Keyword(s):  
Mast Cells ◽  
Vasoactive Intestinal Peptide ◽  
Human Skin ◽  
Nerve Fibres
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